Review
Engineering, Biomedical
Andreas Weltin, Jochen Kieninger
Summary: Electrochemical methods play a crucial role in neural engineering, but are currently underutilized. Research could benefit from more consistent and target-oriented electrochemical methodology and instrumentation. Standardization, consistency, and comparability will be key in translating microtechnology into biomedical and clinical applications.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Electrochemistry
Inge Siegl, Norbert Sailer, Christoph Steffan
Summary: There is currently no standardized practice for presenting compliance voltage data in 3-electrode potentiostatic measurements. This paper proposes a method to compactly display all electrical information by introducing a six region voltage plot and plotting the cell current in the third dimension. The proposed method reveals compliance voltage demands, fosters understanding of a sensor's electric behavior, enables comparison of potentiostat capabilities, and standardizes results across potentiostat platforms.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Amisha Kushwaha, Gajendar Singh, Manu Sharma
Summary: A voltametric and amperometric sensor using CPRGO nanorods was developed for sensitive and selective detection of dopamine. The sensor showed excellent electrochemical performance and high sensitivity in detecting DA in biological fluids.
MICROCHEMICAL JOURNAL
(2021)
Article
Neurosciences
Azariah K. Kaplelach, Stephanie N. Fox, Anna K. Cook, Justin A. Hall, Ryan S. Dannemiller, Karen L. Jaunarajs, Andrew E. Arrant
Summary: This study found that microglia and neurons are the main sources of progranulin in the mouse mPFC. Additionally, the level of ISF progranulin can be increased through LPS treatment, suggesting a role for extracellular progranulin in regulating inflammation. Changes in neuronal activity did not significantly affect ISF progranulin levels.
NEUROBIOLOGY OF DISEASE
(2023)
Review
Chemistry, Analytical
Muhammad Afiq Abdul Ghani, Anis Nurashikin Nordin, Munirah Zulhairee, Adibah Che Mohamad Nor, Mohd Shihabuddin Ahmad Noorden, Muhammad Khairul Faisal Muhamad Atan, Rosminazuin Ab Rahim, Zainiharyati Mohd Zain
Summary: With the increase of zoonotic diseases, there is a demand for improved screening and diagnostic methods. Electrochemical biosensors offer a promising solution as they can be used for on-site testing, reducing the reliance on centralized labs and providing accurate results. Portable potentiostats and microfluidics chambers are also discussed as alternatives to centralized laboratory settings.
Article
Chemistry, Analytical
Rosalia Gonzalez Brito, Pablo Montenegro, Alicia Mendez, Valentina Carabelli, Giulia Tomagra, Ramtin E. Shabgahi, Alberto Pasquarelli, Ricardo Borges
Summary: Platelets are a good material to study exocytosis and have been used to investigate neurological and psychiatric diseases. The release of serotonin by platelets can be observed directly using carbon fiber electrodes. However, this method is tedious due to the limited number of secretory granules released by each platelet.
Article
Chemistry, Analytical
Sinem Evli, Deniz Aktas Uygun
Summary: One-dimensional Ni/Au/PPy-COOH nanowires with multiple segments were synthesized and their smooth surfaces and magnetic properties were investigated. These nanowires were used to modify the electrode surface and as a micro-environment for Trametes versicolor laccase. The biosensor based on this design demonstrated high sensitivity, excellent selectivity, wide linear range, and low LOD for the detection of dopamine in biological samples.
Article
Chemistry, Analytical
Marsilea A. Booth, Sally A. N. Gowers, Melinda Hersey, Isabelle C. Samper, Seongjun Park, Polina Anikeeva, Parastoo Hashemi, Molly M. Stevens, Martyn G. Boutelle
Summary: The study explored the potential of polymer fibers as a platform for sensing pH and neurometabolic lactate, successfully detecting transient lactate changes in an in vivo mouse model. The changes in lactate concentration were associated with spreading depolarizations.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jacobus Frederick van Staden, Raluca-Ioana Stefan-van Staden
Summary: This study developed graphene-based amperometric microsensors for detecting dopamine in urine samples, with high sensitivity and good recovery rate of dopamine, aiming at diagnosing neurological disorders in the elderly.
ANALYTICAL LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Brianna E. George, Monica H. Dawes, Emily G. Peck, Sara R. Jones
Summary: Chronic heroin exposure leads to low dopamine levels in the nucleus accumbens and alterations in dopamine release dynamics, which may contribute to increased heroin seeking.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Zachary A. Rodd, Eric A. Engleman, William A. Truitt, Andrew R. Burke, Andrei Molosh, Richard L. Bell, Sheketha R. Hauser
Summary: The chemogenetic procedure DREADD selectively affects protein receptors. Administering clozapine N-oxide (CNO) significantly increases levels of glutamate and dopamine in the medial prefrontal cortex (mPFC). However, caution should be taken as CNO is not an inert compound, and the effects of clozapine and N-desmethylclozapine (N-Des) in CNO-DREADD studies need to be controlled.
Article
Chemistry, Analytical
Sofwan Nuh, Apon Numnuam, Panote Thavarungkul, Tonghathai Phairatana
Summary: This study developed a novel microfluidic-based electrochemical sensor for continuous dopamine detection. By integrating an electrochemical sensor into a microfluidic chip, real-time chemical monitoring with low analyte consumption, short analysis time, low cost, and compact size was achieved. The sensor exhibited high sensitivity, selectivity, and stability, making it a powerful tool for continuous dopamine detection in human clinical applications.
Review
Biochemistry & Molecular Biology
Yann S. Mineur, Marina R. Picciotto
Summary: The neurotransmitter acetylcholine (ACh) plays a crucial role in regulating various cognitive and behavioral processes. Measurement of ACh dynamics in the brain is essential for understanding the neural mechanisms underlying these processes. This review discusses several techniques for measuring ACh levels in the brain in vivo, with a focus on genetically encoded fluorescent sensors coupled with fiber photometry. These sensors provide a means for accurately measuring ACh release in awake, behaving animals, contributing to a better understanding of cholinergic modulation of complex behaviors.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Mareike Herrmann, Aya Abdalla, Bhavik Anil Patel
Summary: This study demonstrates that dopamine calibrations are highly dependent on potassium ion concentration, while serotonin is less affected. Therefore, when interpreting and comparing measurements between different studies, it is important to consider this factor.
Article
Environmental Sciences
Malwina Lisek, Tomasz Boczek, Joanna Stragierowicz, Julia Wawrzyniak, Feng Guo, Michal Klimczak, Anna Kilanowicz, Ludmila Zylinska
Summary: PCN67 is considered the most toxic congener among polychlorinated naphthalenes, affecting human health by causing hepatotoxicity and disrupting reproductive, endocrine, and hematological systems. Studies have shown that PCN67 disturbs dopaminergic transmission by reducing dopamine synthesis, affecting the expression of antioxidant enzymes, and altering vesicular monoamine transporter 1 expression.
Article
Chemistry, Analytical
Keeley L. Baker, Fiachra B. Bolger, Michelle M. Doran, John P. Lowry
Article
Multidisciplinary Sciences
Salvatore Pisanu, Carla Cacciotto, Daniela Pagnozzi, Giulia Maria Grazia Puggioni, Sergio Uzzau, Paolo Ciaramella, Jacopo Guccione, Martina Penati, Claudia Pollera, Paolo Moroni, Valerio Bronzo, Maria Filippa Addis
SCIENTIFIC REPORTS
(2019)
Article
Veterinary Sciences
Giulia Maria Grazia Puggioni, Vittorio Tedde, Sergio Uzzau, Jacopo Guccione, Paolo Ciaramella, Claudia Pollera, Paolo Moroni, Valerio Bronzo, Maria Filippa Addis
RESEARCH IN VETERINARY SCIENCE
(2020)
Article
Microbiology
Giulia Maria Grazia Puggioni, Vittorio Tedde, Sergio Uzzau, Simone Dore, Manuele Liciardi, Eugenia Agnese Cannas, Claudia Pollera, Paolo Moroni, Valerio Bronzo, Maria Filippa Addis
Article
Neurosciences
John Kealy, Carol Murray, Eadaoin W. Griffin, Ana Belen Lopez-Rodriguez, Daire Healy, Lucas Silva Tortorelli, John P. Lowry, Leiv Otto Watne, Colm Cunningham
JOURNAL OF NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Maria Antonietta Dettori, Davide Fabbri, Alessandro Dessi, Roberto Dallocchio, Paola Carta, Claudia Honisch, Paolo Ruzza, Donatella Farina, Rossana Migheli, Pier Andrea Serra, Roberto A. Pantaleoni, Xenia Fois, Gaia Rocchitta, Giovanna Delogu
Article
Agriculture, Dairy & Animal Science
L. Wollowski, W. Heuwieser, A. Kossatz, M. F. Addis, G. M. G. Puggioni, Laurent Meriaux, S. Bertulat
Summary: The timely and objective diagnosis and classification of mastitis is crucial, and analyzing specific biomarkers in milk can be advantageous in comparison to other criteria. The study aimed to investigate the diagnostic value of 3 biomarkers in detecting subclinical and clinical mastitis, showing the potential for reliable detection using cathelicidin, milk amyloid A, and haptoglobin measurements in milk.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Andrea Bacciu, Paola Arrigo, Rossana Migheli, Alessandra T. Peana, Gaia Rocchitta, Pier Andrea Serra
Summary: This study analyzed the impact of glycerol and triethylene glycol as enzyme stabilizers, combined with long-term storage at low temperatures, on biosensor performance. The results showed that both glycols were capable of increasing enzymatic activity and maintaining good biosensor efficiency over time. Glycerol was more effective in stabilizing the enzyme and maintaining analytical performance when the biosensors were stored at -20 degrees C, while triethylene glycol performed the same function at -80 degrees C.
Article
Chemistry, Analytical
Ylenia Spissu, Antonio Barberis, Gianfranco Bazzu, Guy D'hallewin, Gaia Rocchitta, Pier Andrea Serra, Salvatore Marceddu, Claudia Vineis, Sebastiano Garroni, Nicola Culeddu
Summary: The study showed that functionalized carbon screen-printed sensors have better performance, enhancing the measurement sensitivity of ascorbic acid concentration and reducing the detection limit. Functionalization of HRCM and MWCNTs resulted in increased electroactive area and improved diffusion rate of AA molecules towards the electrode surface.
Article
Chemistry, Analytical
Kobi P. Bermingham, Michelle M. Doran, Fiachra B. Bolger, John P. Lowry
Summary: The performance optimization of a polymer/enzyme composite biosensor for monitoring glutamate was conducted in vitro. The biosensor displayed appropriate sensitivity, stability, and biocompatibility, and reliably detected glutamate in neurological monitoring. Implantation experiments in rats demonstrated stable recording and reliable detection of physiological changes in glutamate in response to behavioral/neuronal activation.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Gaia Rocchitta, Carla Rozzo, Marina Pisano, Davide Fabbri, Maria Antonietta Dettori, Paolo Ruzza, Claudia Honisch, Roberto Dallocchio, Alessandro Dessi, Rossana Migheli, PierAndrea Serra, Giovanna Delogu
Summary: This study synthesized three curcumin-inspired compounds and analyzed their activities. The results showed that these compounds have inhibitory effects on melanin synthesis and have high antioxidant activity.
Article
Chemistry, Analytical
Myriam Caval, Carla Sanna, Salvatore Marceddu, Gaia Rocchitta, Pier Andrea Serra
Summary: This paper investigates the possibility of using carbonaceous support materials for platinum deposition and evaluates the sensitivity of hydrogen peroxide and ascorbic acid using CV and CPA techniques. The results suggest that 0.3 mm pencil leads have the potential for constructing glucose biosensors.
Article
Biochemistry & Molecular Biology
Myriam Caval, Maria Antonietta Dettori, Paola Carta, Roberto Dallocchio, Alessandro Dessi, Salvatore Marceddu, Pier Andrea Serra, Davide Fabbri, Gaia Rocchitta
Summary: This paper evaluates the performances of electrosynthesized polymers, layered by means of constant potential amperometry, for amperometric biosensors. The polymeric films of zingerone and its dimer dehydrozingerone showed excellent shielding characteristics against interfering species and an increased monitoring capability for hydrogen peroxide. Docking analyses and theoretical speculation on the molecular structures of the obtained polymers were also conducted.
Article
Chemistry, Analytical
Andrea Bacciu, Paola Arrigo, Giovanna Delogu, Salvatore Marceddu, Patrizia Monti, Gaia Rocchitta, Pier Andrea Serra
Article
Chemistry, Analytical
Gama Theophile Gnahore, Jack L. Kelly, Saidhbhe L. O'Riordan, Fiachra B. Bolger, Michelle M. Doran, Michelle Sands, John P. Lowry
ANALYTICAL METHODS
(2020)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)