Journal
ANALYTICAL BIOCHEMISTRY
Volume 381, Issue 1, Pages 8-17Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.07.001
Keywords
hormone oscillations; pulsatility; luteinizing hormone; deconvolution analysis; software
Funding
- National Institutes of Health (NIH) [RR-00847, R01 RR019991, R25 DK064122, R21 DK072095, P30 DK063609, K25 HD001474, R01 DK51562, R01 DK076037]
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Hormone signaling is often pulsatile, and multiparameter deconvolution procedures have long been used to identify and characterize secretory events. However, the existing programs have serious limitations, including the subjective nature of initial peak selection, lack of statistical verification of presumed bursts, and user-unfriendliness of the application. Here we describe a novel deconvolution program, AutoDecon, which addresses these concerns. We validate AutoDecon for application to serum luteinizing hormone (LH) concentration time series using synthetic data mimicking real data from normal women and then comparing the performance of AutoDecon with the performance of the widely employed hormone pulsatility analysis program Cluster. The sensitivity of AutoDecon is higher than that of Cluster (similar to 96% vs. 80%, P = 0.001 However, Cluster had a lower false-positive detection rate than did AutoDecon (6% vs. 1%, P = 0.001 Further analysis demonstrated that the pulsatility parameters recovered by AutoDecon were indistinguishable from those characterizing the synthetic data and that sampling at 5- or 10-min intervals was optimal for maximizing the sensitivity rates for LH. Accordingly, AutoDecon presents a viable nonsubjective alternative to previous pulse detection algorithms for the analysis of LH data. it is applicable to other pulsatile hormone concentration tin-we series and many other Pulsatile phenomena. The software is free and downloadable at http://mljohnson.pharm.virginia.edu/home.html. (c) 2008 Elsevier Inc. All rights reserved.
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