Article
Chemistry, Analytical
Fatemeh Khosravi, Mahdi Rahaie, Mohammad Reza Ghaani, Mostafa Azimzadeh, Ebrahim Mostafavi
Summary: Electrochemical nanobiosensors have revolutionized medical diagnosis with their enhanced sensitivity provided by the utilized nanomaterials. In this study, a novel sensitive electrochemical nanobiosensor for miR-155, a biomarker for early breast cancer detection, was introduced. The nanobiosensor utilized a combination of Fe-Ni@rGO, GQD-Ag, and GNS nanomaterials to expand the surface area and conductivity of the electrode, resulting in high sensitivity and specificity for miR-155 detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Inorganic & Nuclear
Abin Philip, A. Ruban Kumar
Summary: The necessity for the development of highly sensitive and selective detection techniques for the investigation of biomolecules is inevitable. Surface plasmon resonance (SPR) based biosensor has evolved as a sustainable and commercially applicable tool for this investigation. Exploring real-time, cost-effective, and time-efficient detection techniques is crucial for improving quality of life.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Jeonghun Kim, So Yeon Ahn, Soong Ho Um
Summary: This study presents a novel DNA nanostructure for detecting cancer miRNA variants with temperature insensitivity and high selectivity. By optimizing parameters and considering thermodynamic stability, design guidelines for new sensing systems of miRNA variation were provided.
Article
Biotechnology & Applied Microbiology
Sahar Mohajeri, Saeed Moayedi, Leila Azimi, Mohammad Akrami, Mazda Rad-Malekshahi, Mohammad Reza Fazeli, Fatemeh Fallah, Ismaeil Haririan
Summary: Streptococcal pharyngitis, caused by Streptococcus pyogenes, is a common disease that can lead to heart problems if untreated. Accurate diagnosis is challenging due to the similarity in symptoms with viral pharyngitis. Researchers have developed a nanobiosensor based on the sugar code-lectin interaction, which can rapidly and accurately detect streptococcal pharyngitis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Min Sun, Zhenkai Ji, Le He, Chuanzhen Zhao, Liang Ma, Xiuzhen Xu, Erik Jan Cornel, Zhen Fan, Xiaobin Xu
Summary: The study demonstrates a rapid and efficient intracellular delivery method for miRNA against esophageal cancer via photothermal effects induced on plasmonic pyramid arrays. This approach achieves successful and quick delivery of miRNA and combats drug resistance.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Azam Ahangari, Pezhman Mahmoodi, Abdolmajid Mohammadzadeh
Summary: Zoonosis refers to infectious diseases transmitted between animals and humans. Rapid and accurate detection of bacterial pathogens is crucial for preventing the spread of these diseases. Conventional diagnostic methods have limitations in terms of time, cost, and equipment requirements. Biosensors, empowered by nanoparticles, have shown promise as a simple, innovative, and cost-effective tool for detecting zoonotic bacteria.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Peng Shen, Qinyu Ge, Yihan Zhao, Mingsong Zhu, Ting Huang, Qingjiang Sun
Summary: This study reports the construction of a core-shell-nanostructured dual-emission quantum dot-loaded cerasome spherical nucleic acid that acts as an intracellular nanobiosensor. The nanobiosensor uses AND gate-guided dual-DNAzyme walker-mediated resonance energy transfer to detect dual endogenous markers and differentiate cancer/normal cells and cancer cell subtypes with improved imaging contrast and accuracy.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Meng-lin Huang, Yan-lin Wang, Xue-lian Xiang, Ya-li Yuan, Ruo Yuan, Sha-ping Wei
Summary: In this study, a novel AuNPs@Bi2S3/Bi2O3 nanocomposite was used to construct an ultra-sensitive photoelectrochemical (PEC) sensing platform for miRNA-21 detection. By controlling the energy levels and surface plasmon resonance effect, the photoelectric conversion efficiency was enhanced and abundant dsDNA and mimic enzyme MnPP were generated. The developed biosensor exhibited an ideal linear relationship and a low detection limit, which is significant for bioanalysis and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Emadoddin Amin Sadrabadi, Ali Benvidi, Amin Shiralizadeh Dezfuli, Leila Asgharnejad, Maryam Daneshpour, Mostafa Azimzadeh, Patricia Khashayar
Summary: The unique micro-RNA signatures of different tumours distinguish cancer from normal tissue. A nanobiosensor based on microRNA complementary strand hybridization was proposed to accurately and specifically identify biomarkers for breast cancer diagnosis. The nanobiosensor demonstrated a low detection limit of 0.1 fM and a dynamic range of 0.3 fM-500 pM. It showed specificity to the target miRNA sequence and performed well in evaluating real samples without interference.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Physical
Yingwen Chen, Xuanqun Wang, Xing Na, Yingkun Zhang, Zan Li, Xiaohui Chen, Linfeng Cai, Jia Song, Ren Xu, Chaoyong Yang
Summary: Single-cell microRNA (miRNA) sequencing provides comprehensive insights into the abundance and complex networks of miRNAs and their dynamic regulation of cellular events. However, current benchtop-based technologies for single-cell miRNA sequencing have limitations in terms of throughput, efficiency, manual operations, and reagent costs. In this study, a highly multiplexed and automated sample preparation platform called Hiper-seq is introduced, which is based on digital microfluidics (DMF). The platform allows for the construction of miRNA sequencing libraries through digital control of addressable droplets on the DMF chip, enabling selective isolation and parallel processing of multiple single cells. This platform offers higher throughput and efficiency for single-cell miRNA measurement, with a smaller reaction volume and lower reagent cost compared to benchtop methods. Furthermore, it has been successfully applied to explore miRNAs involved in the ossification of mouse skeletal stem cells after bone fracture, leading to the discovery of previously unreported miRNAs that regulate bone repairing.
Article
Chemistry, Analytical
Min Yu Choi, Dong Hyeok Park, Jin-Ha Choi
Summary: A highly sensitive nanobiosensor based on metal-enhanced fluorescence was developed to measure multiple miRNAs. Porous gold nanorods with a wide absorption wavelength were synthesized to extend the plasmonic-based MEF phenomenon to various fluorescent materials. This method allows sensitive and selective measurement of two different miRNAs simultaneously and can be used for early diagnosis of cancer and other nucleic acid-related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Oncology
Goknur Kara, Banu Arun, George A. Calin, Bulent Ozpolat
Summary: The discovery of microRNAs has revolutionized cancer treatment and their effective and safe delivery remains a challenge. New nanoparticle-based carriers have enabled successful tumor-targeted delivery of miRNA therapeutics, accelerating the transition of miRNAs from the lab to the bedside.
Article
Chemistry, Multidisciplinary
Haoyuan Cai, Mengwei Wang, Zhuohui Wu, Jing Liu, Xiaoping Wang
Summary: We propose a high-sensitivity SPR biosensor based on a multilayer structure consisting of Au, MoS2, and graphene. Numerical simulations and experiments demonstrate that the sensor has a sensitivity about 2 times higher than the conventional Au-based SPR sensor, and the presence of MoS2 enhances the sensitivity. The fabricated sensor shows a very low limit of detection for okadaic acid.
Review
Biochemistry & Molecular Biology
Yinghao Li, Qingwei Liao, Wei Hou, Lei Qin
Summary: The control of surface plasmon using metal micro-nano structures and metal/material composites can generate novel phenomena such as optical nonlinear enhancement, transmission enhancement, orientation effect, high sensitivity to refractive index, negative refraction, and dynamic regulation of low threshold. These phenomena have significant potential in fields such as nano-photonics, super-resolution imaging, energy, sensor detection, and life science. Silver nanoparticles, with their high sensitivity to refractive index change, convenient synthesis, and controllable shape and size, are commonly used in surface plasmon sensors. This review summarizes the basic concept, fabrication, and applications of silver-based surface plasmon sensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Electrical & Electronic
Amena Salim, Ritu Vishnoi, Himanshu Dixit, Vikesh Chaudhary, Jyotsna Bhardwaj, Divya Gupta, Sanjeev Aggarwal, Umesh Kumar Dwivedi, Pushpendra Kumar, Sunita Bishnoi, Ganesh D. Sharma, Rahul Singhal
Summary: Au nanoparticles are synthesized in a fullerene C-60 matrix and their SPR wavelength is tunable by ion irradiation. The dose of the ion beam affects the shift of SPR wavelength and the growth of nanoparticles. Raman spectroscopy confirms the transformation of the fullerene C-60 matrix into amorphous carbon at higher doses, resulting in a blue shift of SPR wavelength.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2022)
Review
Biochemistry & Molecular Biology
Francesca Roda, Riccardo Caraffi, Silvia Picciolini, Giovanni Tosi, Maria Angela Vandelli, Barbara Ruozi, Marzia Bedoni, Ilaria Ottonelli, Jason Thomas Duskey
Summary: Nanoparticles have potential as a strategy to improve the treatment of glioblastoma by enhancing drug delivery and targeting specific receptors or pathways. This review explores the latest strategies for drug delivery to glioblastoma, with a focus on the importance of surface functionalization for blood-brain barrier crossing and tumor specific targeting.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Simone Agostini, Roberta Mancuso, Lorenzo Agostino Citterio, Gabriela Alexandra Mihali, Beatrice Arosio, Mario Clerici
Summary: The concentration of miR-451a in serum was significantly increased in frail patients, while the concentrations of miR-93-5p and miR-495-3p were reduced, and miR-155-5p was increased. The multicomponent exercise program was able to improve physical performance and muscle strength in frail patients and modulate the expression of miRNAs in serum.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Simone Agostini, Elisabetta Bolognesi, Roberta Mancuso, Ivana Marventano, Lorenzo Agostino Citterio, Franca Rosa Guerini, Mario Clerici
Summary: The concentration of specific miRNAs (miR-27b-3p, miR-181a-5p, and miR-23a-3p) was found to be associated with a SNAP-25 polymorphism. In vitro experiments confirmed that miR-181a-5p and miR-23a-3p regulate SNAP-25 gene and protein expression, while miR-27b-3p does not. These findings suggest the potential use of miR-181a-5p and miR-23a-3p as biomarkers or therapeutic targets for diseases involving altered SNAP-25 expression.
Article
Biology
Valentina Mangolini, Alice Gualerzi, Silvia Picciolini, Francesca Roda, Angela Del Prete, Luana Forleo, Rudy Alexander Rossetto, Marzia Bedoni
Summary: Extracellular vesicles (EVs) isolated from saliva can be comprehensively characterized by Raman spectroscopy, providing valuable biomarkers for diagnosis. EVs from blood are difficult to separate from other particles and proteins, while saliva EVs can be easily analyzed. Despite the low concentration, Raman spectroscopy is capable of accurately characterizing salivary EVs. This suggests the potential of salivary EVs for diagnostic purposes, with Raman spectroscopy playing a key role.
Article
Biochemistry & Molecular Biology
Francesca La Rosa, Simone Agostini, Federica Piancone, Ivana Marventano, Ambra Hernis, Chiara Fenoglio, Daniela Galimberti, Elio Scarpini, Marina Saresella, Mario Clerici
Summary: Alzheimer's Disease (AD) is the most common form of dementia, characterized by the deposition of neurotoxic plaques composed of amyloid-beta (Ab). Peripheral inflammation has been identified as an early event in AD pathogenesis. This study focuses on TREM2, a receptor that plays a role in immune cell function and may serve as a potential peripheral biomarker for AD diagnosis and prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Francesca Roda, Silvia Picciolini, Valentina Mangolini, Alice Gualerzi, Pierfausto Seneci, Antonio Renda, Silvia Sesana, Francesca Re, Marzia Bedoni
Summary: Raman Spectroscopy (RS) is proposed as a valuable tool for characterizing liposomes (LPs) and evaluating their effectiveness and safety in treating neuroinflammation and microglial dysfunctions in Glioblastoma multiforme and Alzheimer's disease. The Raman spectra of different LP components contribute to the overall LP Raman fingerprint, making it possible to differentiate LPs with different functionalization patterns. Raman data analysis allows for the evaluation of LP synthesis efficacy using small sample amounts.
Article
Biochemistry & Molecular Biology
Lorenzo Agostino Citterio, Roberta Mancuso, Simone Agostini, Mario Meloni, Mario Clerici
Summary: The etiology of Parkinson's disease is still unclear, but it is likely to involve both genetic and environmental factors. Studies have found dysregulated microRNA expression, specifically increased levels of miR-7-1-5p and miR-223-3p in the serum of Parkinson's patients, suggesting that these microRNAs could potentially serve as diagnostic biomarkers.
Article
Chemistry, Multidisciplinary
Beatriz Torres-Herrero, Ilaria Armenia, Maria Alleva, Laura Asin, Sonali Correa, Cecilia Ortiz, Yilian Fernandez-Afonso, Lucia Gutierrez, Jesus M. de la Fuente, Lorena Betancor, Valeria Grazu
Summary: Here, researchers have developed nanohybrids (nHs) for remote activation of a therapeutic enzyme in Directed Enzyme Prodrug Therapy (DEPT). The nanosized hybrids, composed of magnetic nanoparticles (MNPs) and horseradish peroxidase (HRP) encapsulated in biomimetic silica, were optimized for efficient remote activation of the therapeutic enzyme. The application of alternating magnetic fields (AMFs) to MNPs increased the bioconversion rate of HRP, demonstrating the possibility of enzyme nanoactuation with co-encapsulated MNPs. In vitro and in vivo experiments showed that the enzyme-loaded nHs triggered cell death and significantly reduced tumor volume growth when exposed to AMFs, highlighting the potential of this spatiotemporally controlled DEPT strategy to overcome off-target effects.
Article
Pharmacology & Pharmacy
Meixiang Huang, Carolyn Tallon, Xiaolei Zhu, Kaitlyn D. J. Huizar, Silvia Picciolini, Ajit G. Thomas, Lukas Tenora, Wathsala Liyanage, Francesca Roda, Alice Gualerzi, Rangaramanujam M. Kannan, Marzia Bedoni, Rana Rais, Barbara S. Slusher
Summary: In this study, the researchers used improved nanoparticles to deliver the inhibitor DPTIP and found its potential in inhibiting protein propagation in Alzheimer's disease. Further investigation revealed that the inhibitor only showed effect through its targeting of microglial cells. This highlights the importance of understanding cell-specific mechanisms when designing targeted therapies for Alzheimer's disease.
Letter
Oncology
Carlo Morasso, Renzo Vanna, Francesca Piccotti, Lidia Frizzi, Marta Truffi, Sara Albasini, Camelia Borca, Thomas Huthwelker, Laura Villani, Oliver Bunk, Cinzia Giannini, Fabio Corsi
CANCER COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Silvia Picciolini, Valentina Mangolini, Francesca Roda, Angelo Montesano, Francesca Arnaboldi, Piergiuseppe Liuzzi, Andrea Mannini, Marzia Bedoni, Alice Gualerzi
Summary: The lack of specific measurable markers of recovery makes it difficult to predict the response of stroke patients to treatment. This study identified a panel of Extracellular Vesicle (EV)-associated molecules that could be used to profile stroke patients at the beginning of rehabilitation and predict their response to treatment. The increased expression of vascular endothelial growth factor receptor 2 (VEGFR2) and translocator protein (TSPO) on small EVs in the blood of subacute ischemic stroke patients suggests their potential as tools for predicting recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Marina Paris Ogayar, Diego Mendez-Gonzalez, Irene Zabala Gutierrez, Alvaro Artiga, Jorge Rubio-Retama, Oscar G. Calderon, Sonia Melle, Aida Serrano, Ana Espinosa, Daniel Jaque, Riccardo Marin
Summary: Luminescence nanothermometry is a technique that uses luminescence signals from nanosized materials to remotely and minimally invasively measure temperature. However, in a biomedical context, the reliability of temperature measurement is compromised by bias caused by environmental conditions. This study reveals an unexpected source of bias induced by metal ions, which enhances the emission of Ag2S nanothermometers. These findings highlight the need for caution when using luminescence nanothermometry in complex biological environments.