Article
Multidisciplinary Sciences
Nam K. Tran, Taylor Howard, Ryan Walsh, John Pepper, Julia Loegering, Brett Phinney, Michelle R. Salemi, Hooman H. Rashidi
Summary: ML-enhanced MALDI-TOF-MS for COVID-19 testing provides a performance-comparable solution that overcomes logistical barriers encountered by current testing paradigms.
SCIENTIFIC REPORTS
(2021)
Article
Medical Laboratory Technology
Ming-Hui Yang, Shih-Cheng Chen, Ko-Chin Chen, Han-Ping You, Hsin-Yi Wu, Yi-Ming Arthur Chen, Ying-Fong Huang, Ming-Yii Huang, Cheng-Hui Yuan, Po-Chiao Lin, Yu-Chang Tyan
Summary: The study introduced a method to quantify progesterone concentration in human serum based on isotope dilution mass spectrometry. By extracting and cleaning serum samples, combined with mass spectrometry, progesterone concentration could be accurately determined. Results demonstrated that this method showed good robustness and consistency in measuring progesterone levels.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Huifang Zhao, Jie Wang, Huayu Zhao, Yulong Liu, Yanqiu Li, Ruiping Zhang
Summary: By synthesizing N and B dual-doped 3D carbon nanofibers as MALDI matrix, the surface area, functional groups, and active sites were enhanced, leading to improved laser absorption and sample enrichment capabilities. This novel matrix demonstrated high performance in analyzing various small molecules, showing excellent signal to noise ratio and broad linear ranges for molecules like glucose, as well as successful application in identifying and analyzing saccharides in natural honey through MALDI-TOF MS.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Lijun Zhao, Lirong Jia, Bingcun Ma, Wei Zhong, Ying Huang, Feixia Duan
Summary: The study found that temperature significantly affects the texture stiffening of soybean curd, with high-temperature autoclaving leading to increased hardness. Analysis identified Bacillus, Clostridium, Lysinibacillus, and Brevibacillus as main contaminants. Bacterial contamination in two different processing lines was dominated by Staphylococcus, Macrococcus, Psychrobacter, Bacillus, and Acinetobacter.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Environmental
Qiyue Zhao, Han Li, Hui Chen, Chao Wu, Hesham EI-Seedi, Xianbing Xu, Ming Du
Summary: The study successfully demonstrated rapid detection of a batch of samples using plasmonic nanoshell enhanced MALDI-TOF MS technology, providing a new method for high-throughput metabolomics and clinical diagnosis.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
You-Ran Jang, Sewon Kim, Jae-Ryeong Sim, Su-Bin Lee, Sun-Hyung Lim, Chon-Sik Kang, Changhyun Choi, Tae-Won Goo, Jong-Yeol Lee
Summary: This study analyzed the HMW-GS composition of 665 wheat genotypes in South Korea using MALDI-TOF-MS, identifying multiple alleles with distinct effects on processing properties. By determining Glu-1 quality score and selecting wheat lines with the 1Bx7(OE) subunit, the study provided important data support for the development of high-quality wheat. The MALDI-TOF-MS method was demonstrated to be fast, accurate, reliable, and effective in analyzing large numbers of wheat germplasms or breeding lines in a high-throughput manner.
Article
Biotechnology & Applied Microbiology
Eiseul Kim, Eun-Ji Cho, Seung-Min Yang, Mi-Ju Kim, Hae-Yeong Kim
Summary: A study compared culture-dependent and culture-independent methods for identifying microbial community changes during kimchi fermentation. Both methods identified Lactobacillus-related genera, Leuconostoc, and Weissella as predominant microorganisms, with the culture-independent method additionally identifying non-lactic acid bacteria and yeasts. The culture-dependent method could identify species level and predominant species in viable cell communities, while culture-independent method provided a more comprehensive view of the microbial ecology in fermented kimchi.
Article
Agricultural Engineering
Katarzyna Rafinska, Olga Wrona, Aneta Krakowska-Sieprawska, Justyna Walczak-Skierska, Anna Kielbasa, Zbigniew Rafinski, Pawel Pomastowski, Mateusz Kolankowski, Boguslaw Buszewski
Summary: Enzyme-assisted extraction of plant material is a widely used method that allows for the isolation of biologically active compounds. This study aimed to examine the relationship between the efficiency of enzymatic hydrolysis of plant material and the quality of the obtained extracts. The results showed that pectinase was particularly effective in obtaining high-quality extracts with low levels of interfering compounds, resulting in extracts with high utility value.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Review
Medicine, Research & Experimental
Maria Emilia Duenas, Rachel E. Peltier-Heap, Melanie Leveridge, Roland S. Annan, Frank H. Buttner, Matthias Trost
Summary: High-throughput screening is a key method in early drug discovery. Recent developments in mass spectrometry and automation have revolutionized its application, allowing for label-free assays that are cheaper, faster, and more physiologically relevant than traditional methods. This review discusses the current techniques, advantages, and disadvantages of mass spectrometry in drug discovery, as well as its potential in multiplexed cellular assays.
EMBO MOLECULAR MEDICINE
(2023)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Fisheries
Alexandra Rahmani, Maaike Vercauteren, Katleen Vranckx, Filip Boyen, Adeline Bidault, Vianney Pichereau, Annemie Decostere, Christine Paillard, Koen Chiers
Summary: The study investigated the variability of 27 V. tapetis isolates from bivalves and fish, examining their virulence, gene presence, and protein profiles. Results revealed differences in potential pathogenicity against Manila clams among isolates from different sources.
Article
Microbiology
Eloise J. Busby, Ronan M. M. Doyle, Clara Leboreiro Babe, Kathryn A. A. Harris, Damien Mack, Gema Mendez-Cervantes, Denise M. M. O'Sullivan, Vicky Pang, Zahra Sadouki, Priya Solanki, Jim F. F. Huggett, Timothy D. D. McHugh, Emmanuel Q. Q. Wey
Summary: Managing the transmission of antimicrobial resistance requires reliable methods for tracking outbreaks. MALDI-TOF MS was compared with orthogonal approaches for strain typing, including WGS and FTIR, for Acinetobacter baumannii isolates associated with a healthcare-associated infection event. Reliable molecular typing methods can help to trace transmission routes and manage outbreaks.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Liangqiang Chen, Wenjing Gao, Xue Tan, Ying Han, Fu Jiao, Bin Feng, Jinghang Xie, Bin Li, Huilin Zhao, Huabin Tu, Shaoning Yu, Li Wang
Summary: MALDI-TOF MS is a method that can be used to classify specific model microbiota with high accuracy. The actual MS spectrum of the model microbiota shows a specific spectral fingerprint, which enhances the specificity and accuracy of microbiota classification.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Analytical
Jan Schejbal, Sarka Sefrana, Roman Reminek, Zdenek Glatz
JOURNAL OF SEPARATION SCIENCE
(2019)
Article
Biochemical Research Methods
Antonin Bednarik, Marketa Machalkova, Eugene Moskovets, Katerina Coufalikova, Pavel Krasensky, Pavel Houska, Jiri Kroupa, Jarmila Navratilova, Jan Smarda, Jan Preisler
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2019)
Article
Materials Science, Multidisciplinary
Jan Michalek, Karel Stepka, Michal Kozubek, Jarmila Navratilova, Barbora Pavlatovska, Marketa Machalkova, Jan Preisler, Adam Pruska
MICROSCOPY AND MICROANALYSIS
(2019)
Article
Chemistry, Analytical
Marketa Machalkova, Barbora Pavlatovska, Jan Michalek, Adam Pruska, Karel Stepka, Tereza Necasova, Katarzrna Anna Radaszkiewicz, Michal Kozubek, Jan Smarda, Jan Preisler, Jarmila Navratilova
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Antonin Bednarik, Jan Preisler, Dominika Bezdekova, Marketa Machalkova, Michal Hendrych, Jarmila Navratilova, Lucia Knopfova, Eugene Moskovets, Jens Soltwisch, Klaus Dreisewerd
ANALYTICAL CHEMISTRY
(2020)
Article
Oncology
Barbora Pavlatovska, Marketa Machalkova, Petra Brisudova, Adam Pruska, Karel Stepka, Jan Michalek, Tereza Necasova, Petr Benes, Jan Smarda, Jan Preisler, Michal Kozubek, Jarmila Navratilova
FRONTIERS IN ONCOLOGY
(2020)
Article
Chemistry, Analytical
Antonin Bednarik, Vadym Prysiazhnyi, Jan Preisler
Summary: A novel approach for VOCs analysis based on Au+ ion chemical ionization has been proposed, utilizing a commercially available mass spectrometer with ultrahigh resolving power and sub-ppm mass accuracy. The technique demonstrated excellent performance in analyzing various classes of organic molecules, expanding the available methods for VOCs analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
I Jennifer Gomez, Manuel Vazquez Sulleiro, Anna Doleckova, Nadezda Pizurova, Jirina Medalova, Antonin Bednarik, Jan Preisler, David Necas, Lenka Zajickova
Summary: GQDs synthesized using a fast microwave-assisted hydrothermal protocol exhibited emission from NUV-Vis to NIR range, with their structure and chemical composition elucidated using advanced NMR and spectroscopy techniques. The observed multicolor red-shifted fluorescence emission in cell imaging demonstrates the potential application of GQDs as bioimaging carriers, along with their non-cytotoxicity confirmed through ATP assay.
MATERIALS CHEMISTRY FRONTIERS
(2022)
Article
Chemistry, Analytical
Antonin Bednarik, Vadym Prysiazhnyi, Dominika Bezdekova, Jens Soltwisch, Klaus Dreisewerd, Jan Preisler
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Monika Koktava, Jan Valasek, Dominika Bezdekova, Vadym Prysiazhnyi, Barbora Adamova, Petr Benes, Jarmila Navratilova, Michal Hendrych, Petr Vlcek, Jan Preisler, Antonin Bednarik
Summary: In this study, a novel combination of metal oxide laser ionization mass spectrometry imaging (MOLI MSI) technique with off-line lipid derivatization by ozone was presented for the detection of fatty acids (FA) and their carbon-carbon double bond (C=C) positional isomers in biological tissues. The developed technique allows for the visualization of FA distribution and provides additional information about positional isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Pavel Pleskunov, Vadym Prysiazhnyi, Daniil Nikitin, Tereza Kosutova, Miroslav Cieslar, Ivan Gordeev, Zdenek Krtous, Suren Ali-Ogly, Jan Somvarsky, Mariia Protsak, Kateryna Biliak, Ksenia Kishenina, Antonin Bednarik, Milan Dopita, Jan Preisler, Andrei Choukourov
Summary: Precise molecular identification of specific biomarkers in biological tissues is crucial for accurate diagnostics. In this study, we introduce a new method using monoisotopic niobium nanoparticles as imaging matrices, synthesized without wet chemistry. The niobium nanoparticles form an effective matrix for high-resolution imaging of complex biological samples.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Marek Stiborek, Lenka Jindrichova, Stanislava Meliorisova, Antonin Bednarik, Vadym Prysiazhnyi, Jiri Kroupa, Pavel Houska, Barbora Adamova, Jarmila Navratilova, Viktor Kanicky, Jan Preisler
Summary: This study presents a new technique for digital mapping of biomarkers in tissues using infrared laser ablation and counting intact gold nanoparticle tags. The technique demonstrated successful mapping of a proliferation marker in three-dimensional aggregates of colorectal carcinoma cells, generating sharp distribution maps of the specific biomarker. The developed methodology shows high accuracy and potential applications with multielemental mass spectrometers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
I. Jennifer Gomez, Manuel Vazquez Sulleiro, Nadezda Pizurova, Antonin Bednarik, Petr Lepcio, David Holec, Jan Preisler, Lenka Zajickova
Summary: Carbon dots (CDs) exhibit similar photophysical features despite different synthesis protocols. Understanding the structure is crucial for achieving desired properties. This study provides insight into the structure and formation of CDs, identifying the presence of molecular fluorophore species. Density functional theory calculations support the spontaneous formation of a compact network of molecular species. Additionally, the study comprehensively investigates the photoluminescence properties, paving the way for CD-based logic gate fluorescent sensors.
APPLIED SURFACE SCIENCE
(2023)
Article
Biochemical Research Methods
Michal Zalud, Vadym Prysiazhnyi, Antonin Bednarik, Jan Preisler
Summary: In this study, the detection of single silver nanoparticles was achieved using subatmospheric pressure laser desorption/ionization mass spectrometry (LDI MS). The authors discussed the sample preparation, measurement conditions, generated ions, and limiting experimental factors. The results showed that 84 to 95% of the deposited 80 nm silver nanoparticles were detected. The LDI MS platform presented in this study provides an alternative method for imaging the distribution of individual nanoparticles and has the potential for multiplexed mapping of low-abundance biomarkers in tissues.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Engineering, Chemical
Marek Stiborek, Jan Preisler, Masoud Shekargoftar, Viktor Kanicky, Jakub Kelar
HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY
(2018)