Article
Chemistry, Multidisciplinary
Wuyu Mao, Jie Tang, Liqun Dai, Xinyu He, Jie Li, Larry Cai, Ping Liao, Ruotian Jiang, Jingwei Zhou, Haoxing Wu
Summary: A novel strategy has been developed to create highly fluorescent Huaxi-Fluor probes that exhibit significantly increased fluorescence upon forming the bioorthogonal reaction product, pyridazine. These probes have large Stokes shifts and high quantum yields, with emission wavelengths fine-tunable from 556 to 728 nm by manipulating the fluorophore skeleton. Highly photo-stable and biocompatible, these probes are suitable for live cell organelle visualization without washing steps and imaging tumors in live small animals to depths of 500 μm by two-photon excitation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Taiyu Guo, Xinyue Chen, Wangbo Qu, Bin Yang, Ruowei Tian, Zhirong Geng, Zhilin Wang
Summary: A novel fluorescent probe, CySI, was developed to simultaneously distinguish cysteine and homocysteine with excellent selectivity and fast response. This probe could visualize exogenous Cys and Hcy in cells and monitor endogenous Cys fluctuations independently in mitochondria through the red emission channel.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Hongshun Sun, Yijing Du, Xu Chen, Hong Jiang, Yulong Li, Linjiang Shen
Summary: Myelination is a crucial biological process in the vertebrate nervous system, and the detection and quantification of myelin content can aid in the diagnosis and treatment of diseases like multiple sclerosis. Newly developed Nile Red analogs with near-infrared fluorescence have shown promising results in specifically binding to myelinated fibers, with compound 1-MeNHNR identified as a potential near-infrared fluorescence imaging probe.
Article
Chemistry, Analytical
Kuan-Yi Lee, Ju-Chun Hsieh, Chi-An Chen, Wei-Lun Chen, Hsin-Fei Meng, Chia-Jung Lu, Sheng-Fu Horng, Hsiao-Wen Zan
Summary: An ultrasensitive hydrogen sulfide gas sensor based on methylammonium lead perovskite with SCN- dopant in the active layer demonstrates excellent sensing capability in the ppb regime. With a vertical structure providing enlarged operational current, the sensor shows enhanced sensitivity and improved ambient air endurance. Experiments on the effects of relative humidity and light irradiation were conducted, along with proposed preliminary mechanisms.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Junbin Li, Ying Zhou, Lifei Song, Sheng Yang, Qianqian Wang, Yibo Zhou, Xiao-Bing Zhang, Zhihe Qing, Ronghua Yang
Summary: In this study, a nanobeacon sensitive to hydrogen sulfide (H2S) was developed for monitoring in situ antioxidant fluctuation during epilepsy. The nanobeacon revealed that H2S levels were elevated during mild epilepsy but significantly suppressed during severe epilepsy. This research provides important insights into the pathology of epilepsy.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yanxia Li, Wei Quan, Wenhui Song, Jingting Zhan, Weiying Lin
Summary: In this study, a novel near-infrared H2S fluorescent probe, NIR-Ar-H2S, was used to visualize the changes in H2S content in arthritis. The results showed that H2S levels were elevated in the arthritis model compared to normal joints, and decreased after treatment. This finding provides valuable guidance for the diagnosis and therapeutic evaluation of arthritis.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Pratyush Kumar Mishra, Myeong-Gyun Kang, Hakbong Lee, Seungjoon Kim, Subin Choi, Nirmali Sharma, Cheol-Min Park, Jaewon Ko, Changwook Lee, Jeong Kon Seo, Hyun-Woo Rhee
Summary: The developed proximity photo-crosslinking method utilizing blue light has enabled the detection of protein interactomes in live cells. Dynamic changes in the proteome and protein interactions can be observed using this method, which has also been successfully applied in mouse brain tissue. The technique further allows for the identification of host interactomes of specific proteins, showcasing its potential in studying protein interactions at a close range.
Article
Chemistry, Physical
Honggui Wang, Lang Hu, Huihui Zhou, Ya Zhang
Summary: Various reduced graphene oxide supported CuS composites were prepared via a hydrothermal method, with the optimized 0.1RGO-CuS exhibiting excellent photoelectrocatalytic hydrogen evolution performance in acidic media under near-infrared light. The introduction of RGO effectively enhanced charge transfer resistance, leading to improved charge transport rate and electron-hole separation ability in the photoelectrochemical process.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Chemistry, Multidisciplinary
Yangsen Xu, Mingjian Fan, Wenjuan Yang, Yonghao Xiao, Lingting Zeng, Xiao Wu, Qinghua Xu, Chenliang Su, Qianjun He
Summary: Achieving an NIR-active polymeric carbon nitride by incorporating carbon and potassium dopants in a homogeneous manner has shown high photocatalytic activity for hydrogen generation and potential applications in solar energy and cancer therapy.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Physical
Zahrah Ramadlan Mubarokah, Norsuria Mahmed, Mohd Natashah Norizan, Ili Salwani Mohamad, Mohd Mustafa Al Bakri Abdullah, Katarzyna Bloch, Marcin Nabialek, Madalina Simona Baltatu, Andrei Victor Sandu, Petrica Vizureanu
Summary: A silver sulfide (Ag2S) semiconductor photocatalyst film was successfully synthesized using a solution casting method. The study showed that incorporating Ag2S powder into cellulose films reduced the peak intensity of oxygen-containing functional groups, indicating the formation of a composite film. The crystal structure analysis confirmed a monoclinic acanthite Ag2S structure for all prepared samples, and the photocatalytic efficiency of CF/comAg(2)S and CF/syntAg(2)S films was significantly higher compared to bare Ag2S powder under sunlight exposure.
Article
Chemistry, Analytical
Kyoung-Rok Kim, Jinrok Oh, Jong-In Hong
Summary: In this study, a homogenous ECL probe based on an iridium(III) complex was developed for the selective detection of H2S in aqueous media. The probe showed high selectivity, rapid reaction rate, and high sensitivity, making it a promising analytical tool for field monitoring of H2S.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Alexia G. Cosby, Joshua J. Woods, Patrick Nawrocki, Thomas J. Sorensen, Justin J. Wilson, Eszter Boros
Summary: A novel approach to responsive luminescent Tb(III) probes was developed by modulating the antenna excited triplet state energy. A series of pyridyl-functionalized lanthanide complexes were designed, with triplet energies between 25,800 and 30,400 cm(-1). Conversion of Tb(L3) to Tb(L4) resulted in a 500-fold increase in quantum yield, demonstrating the potential of antenna triplet state energy modulation for biocompatible Tb-based optical turn-on probes.
Article
Spectroscopy
Chang Liu, Qiuchen Liu, Songtao Cai, Huan Ding, Song He, Liancheng Zhao, Xianshun Zeng, Jin Gong
Summary: A novel H2S probe, SHCy-H2S, with high selectivity and sensitivity for specific detection of H2S in lysosome has been designed and synthesized in this study. It exhibits marked color changes in naked-eye and fluorescence, providing a potential tool for exploring the function of H2S at subcellular level.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Multidisciplinary
Qing Shen, Leichen Wang, Xiaohong Ruan, Na Li, Weili Wang, Wenjun Wang, Jinjun Shao, Xiaochen Dong
Summary: Photoacoustic (PA) imaging is a promising biomedical imaging technique that combines the advantages of high-contrast optical imaging and deep tissue penetration of acoustic imaging. This review focuses on the recent research progress of stimuli-responsive organic NIR probes for PA imaging, including temperature, pH, redox, metal ion, and enzyme response. The stimuli-responsive mechanisms are highlighted, and the perspectives and challenges are discussed.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yifan He, Wei Hu, Li Chai, Yanying Wang, Xian Wang, Tao Liang, Haiyan Li, Chunya Li
Summary: A cell membrane-targeted near-infrared fluorescent probe, CMCu-H2S, with high selectivity and rapid response rate, was developed for detecting H2S and studying drug resistance and chemotherapy efficacy.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Xu-Chen Feng, Gang Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: This study designed and synthesized three new Golgi markers for monitoring the normal functioning of the Golgi apparatus. The experiments showed that these markers were able to rapidly and accurately label the Golgi apparatus, and they had low cytotoxicity.
Article
Biochemical Research Methods
Zhi-Hao Zhang, Qing Yu, Gang Zhang, Hai-Yan Peng, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: Two chromenoquinoline-based fluorescent probes 1a-b were synthesized and studied, showing good photostability and selectivity after 6 h irradiation, strong absorption and emission in organic solvents, and the influence of alkylation on the optical properties of 1b.
JOURNAL OF FLUORESCENCE
(2023)
Article
Chemistry, Organic
Ming-Sen Zhu, Gang Zhang, Yu-Jie Xu, Ru Sun, Jian-Feng Ge
Summary: In this study, probes 5a-c and 6b were synthesized by introducing quinazolinone into aldehydes with different double bond numbers. These probes exhibited absorption and emission maxima in different polar solvents. Probe 5c showed significant fluorescence enhancement with increased viscosity. Moreover, probes 5a-c and 6b showed pH-sensitive fluorescence intensity enhancement in alkaline solution. Cell experiments demonstrated the ability of probes 5a-b to target mitochondria and probes 5c-6b to target lysosomes.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Hong-Jiao Liu, Gang Zhang, Yu-Jie Xu, Ru Sun, Jian-Feng Ge
Summary: A novel strategy based on host-guest interaction was proposed for fluorescence enhancement in aqueous solution and cell imaging. By modifying the dyes and forming covalent bonds with methylated beta-cyclodextrin, fluorescent supramolecular nanoparticles were formed, leading to improved fluorescence quantum yields.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Spectroscopy
Hai-Yan Peng, Gang Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: In order to detect the level of thiophenols in the environment and biological samples, probes 1a-b were synthesized by introducing the 2,4-dinitrophenyl ether group into diethylcoumarin-salicylaldehyde based compounds. These probes can form host-guest compounds with methylated beta-cyclodextrin, showing significant increase in fluorescence intensity in thiophenols detection. The hydrophobic cavity of methylated beta-cyclodextrin enhances the fluorescence intensity of probes 1a-b and reduces their detection limits to thiophenols, without affecting their selectivity and response time.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Hong-Jiao Liu, Ming-Sen Zhu, Gang Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: A series of viscosity probes targeting different organelles were obtained using a single hemicyanine dye as the matrix structure. These probes had relatively large molar absorption coefficients in the red light region and their fluorescence intensity in glycerol was 35-184 times higher than that in phosphate buffer solution (PBS). Cell staining experiments demonstrated that these probes could target lysosomes, endoplasmic reticulum and mitochondria, and undergo viscosity-detectable changes in the corresponding organelles.
Article
Materials Science, Biomaterials
Hao-Chi Hao, Gang Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: Four 1,8-naphthyridine derivatives (1a-1d) with different organelle targeting abilities were synthesized. The optical properties of the derivatives were characterized, showing a red-shift of fluorescence emission with increasing system polarity. Colocalization imaging experiments confirmed the organelle-targeting capabilities of the derivatives and their ability to monitor organelle polarity fluctuation. This work proposes a new molecular design idea for developing polarity-sensitive fluorescent probes with organelle targeting.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Jing-Yu Zhao, Gang Zhang, Hao-Chi Hao, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: This study presents the discovery of mitochondria probes for real-time monitoring of mitophagy process. The probes showed excellent fluorescence performance and targeting capability, and were able to detect changes in mitochondrial viscosity. These probes have significant potential for investigating dynamic pathological processes related to mitophagy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Organic
Shu-Yi Li, Wen-Li Wang, Chang Wang, Ru Sun, Jian-Feng Ge
Summary: This study evaluates the contribution of host-guest chemistry in fluorescence enhancement under aqueous conditions. Two benzo[a]phenoxazine derivatives with the adamantyl group were synthesized and formed stable complexes with methyl-beta-cyclodextrin. The emissions of these complexes were greatly increased in aqueous solution, with fluorescence quantum yields reaching 11.5-12.6%. Furthermore, they were successfully applied in fluorescence labeling of organelles in HeLa cells.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Applied
Meng-Nan Li, Jing-Yu Zhao, Gang Zhang, Yu-Jie Xu, Jian-Feng Ge, Ru Sun
Summary: This paper reports the design and synthesis of novel nitrogen-containing heterocycle dyes with large conjugated planar structures. These dyes exhibited excellent optical properties and potential biological applications. They showed fluorescence intensity changes and high fluorescence quantum yields in different solvents, with the polarity of the solvents affecting their excited-state lifetime. The dyes demonstrated toxicity towards cancer cells and growth-promoting effects on normal cells. Additionally, they could be used for long-term observation of targeted cellular physiological activity and dynamic monitoring of changes in lipid droplets polarity. The results have important implications for understanding the physiological functions of lipid droplets.
Article
Chemistry, Analytical
Hai-Yan Peng, Gang Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: Polarity and viscosity, two important microenvironment parameters, were studied using polarity-sensitive and viscosity-sensitive probes. These probes demonstrated changes in fluorescence emission and intensity in response to solvent polarity and viscosity, respectively, providing a tool for localization of specific components within cells.
Article
Materials Science, Biomaterials
Hao-Chi Hao, Gang Zhang, Ya-Nan Wang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: In this study, a hemicyanine dye was developed to distinguish cancer cells from normal cells by targeting different organelles. The dye exhibited strong fluorescence in beta-cyclodextrin solution, making it a potential tool for early cancer detection.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)