Review
Medical Laboratory Technology
Philippe Massonnet, Elodie Grifnee, Jordi Farre-Segura, Justine Demeuse, Loreen Huyghebaert, Thomas Dubrowski, Patrice Dufour, Matthieu Schoumacher, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: Immunocapture is a mature method for sample preparation in quantitation of peptides and proteins. This review provides an overview of clinical applications of immunocapture methods, including protocols with and without enzymatic digestion. The advantages and limitations of these approaches are discussed, along with challenges related to mass spectrometer selection. The potential of these techniques in proteomics-based clinical medicine is demonstrated, paving the way for personalized medicine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Robert Zeidler, Ronald Biemann, Uta Ceglarek, Juergen Kratzsch, Berend Isermann, Alexander Gaudl
Summary: Oxygenated androgens (11-OAs) are discussed as potential biomarkers for diagnosing and controlling disorders with androgen excess. However, the current method for quantifying 11-OAs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) requires extensive sample preparation and is not suitable for high-throughput analysis. In this study, an online-solid-phase extraction-LC-MS/MS method was extended and validated to quantify 11-OAs with high throughput, low limits of quantification, and the ability to distinguish between adrenal and gonadal androgens. This validated method is ready to investigate the diagnostic values of 11-OAs in prospective studies and clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Valentin Braun, Hermann Stuppner, Lorenz Risch, Christoph Seger
Summary: Steroid analysis in clinical laboratories is currently dominated by immunoassays, but liquid chromatography coupled to mass spectrometry (LC-MS/MS) has been proven to be a more capable tool with better sensitivity, specificity, and the ability to analyze multiple steroids and metabolites simultaneously, providing more reliable and comprehensive diagnostic information for endocrinologists.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Elise Mathieu, Cecile Duterme, David Fage, Frederic Cotton
Summary: This study evaluated the immunosuppressant drug assay of the Cascadion SM Clinical Analyzer and its incorporation into a core laboratory. The results showed that the Cascadion ISD Panel performed well in terms of analytical performance, workflow aspects, and ease of use by non-specialized staff.
CLINICA CHIMICA ACTA
(2023)
Article
Medical Laboratory Technology
Valentin Braun, Uta Ceglarek, Alexander Gaudl, Joanna Gawinecka, Daniel Mueller, Manfred Rauh, Matthias Weber, Christoph Seger
Summary: This study conducted an interlaboratory comparison of nine steroid analytes using mass spectrometry (LC-MS/MS) technology. The results revealed main analytical problems such as bias, operator errors, and inaccuracy and imprecision at lower measuring intervals. This highlights the importance of intermethod comparison for monitoring the validity of an assay.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Biochemical Research Methods
Jonas Carneiro Cruz, Israel Donizeti de Souza, Fernando Mauro Lancas, Maria Eugenia Costa Queiroz
Summary: Miniaturized liquid chromatography (LC) is recognized as an important analytical method in various research fields, offering higher resolution and lower consumption. However, limitations such as tubing clogging and small sample volumes hinder high throughput and sensitive analyses. Online coupling of sample preparation techniques can overcome these limitations and improve analytical efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Multidisciplinary
Edgar Toth, Maria Balint, Adam Tolgyesi
Summary: This paper discusses the application of the standard method in pesticide analysis in food and highlights the potential for false positive identification of pesticides in certain food matrices. It suggests that traditional identification criteria may not be sufficient and introduces the use of standard addition as a compensatory method during mass spectrometric detection.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Chiara Fania, Marco Bagnati, Marina Albertario, Carlotta Ferraris, Marta Lamonaca, Umberto Dianzani
Summary: The analysis of immunosuppressant drugs using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is considered the gold standard. Automated systems, such as the Thermo Scientific(TM) Cascadion(TM) SM Clinical Analyzer, eliminate manual processes and provide efficient and accurate results. The comparison of LC-MS/MS with the automated system showed a good correlation between the two methods.
APPLIED SCIENCES-BASEL
(2022)
Review
Food Science & Technology
Minke Yang, Juan Li, Chengying Zhao, Hang Xiao, Xiang Fang, Jinkai Zheng
Summary: Flavonoids have gained increasing attention for their various health-promoting effects. Liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS) has become a widely used technique for detecting flavonoids, providing efficient separation and structural information. This review comprehensively summarizes the principles and methodologies of LC-Q-TOF-MS/MS for detecting flavonoids in food, including qualitative and quantitative determination. It also discusses recent applications in agricultural products, food processing, and in vivo metabolism, as well as suggesting technological improvements and potential applications.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Medical Laboratory Technology
Stephanie Rankin-Turner, Liam M. Heaney
Summary: Mass spectrometry (MS) has become a powerful and versatile tool in clinical laboratory, with advancements in biomarker detection, protein measurement, therapeutic drug monitoring, and automation leading to potential revolution in clinical testing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Athina G. Nicolaou, Marios C. Christodoulou, Ioannis J. Stavrou, Constantina P. Kapnissi-Christodoulou
Summary: This review discusses the impact of cannabis abuse on neurocognitive functions and emphasizes the importance of developing analytical methods. The focus is on liquid chromatography mass spectrometry or tandem mass spectrometry as the new golden standard for forensic analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Medical Laboratory Technology
E. G. W. M. Lentjes, H. N. Bui, L. R. Ruhaak, I. P. Kema, K. L. M. Coene, J. M. W. Van den Ouweland
Summary: LC-MS/MS methods have been increasingly used in hospital laboratories over the past decade. This switch from immunoassays to LC-MS/MS methods was driven by the promise of improved sensitivity and specificity, better standardization, and better between-laboratory comparison. However, it remains uncertain whether these expectations have been met.
CLINICA CHIMICA ACTA
(2023)
Article
Pharmacology & Pharmacy
Ying Xia, Jia-Yi Long, Meng-Yuan Shen, Na Dong, Hong-Li Guo, Ya-Hui Hu, Xiao-Peng Lu, Xuan-Sheng Ding, Feng Chen, Jin-Chun Qiu
Summary: The study compared the accuracy of two methods, EMIT assay and LC-ESI-MS/MS, in measuring plasma VPA concentrations for pediatric patients with epilepsy. Results showed a significant correlation between the two methods but with EMIT assay overestimating VPA levels compared to LC-ESI-MS/MS method. Further studies are needed to confirm the optimal therapeutic range for VPA monitoring in clinical practice.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xi Zhang, Zhuoling An, Yali Lv, Guangrun Li, Lihong Liu, Pengfei Li
Summary: A rapid and efficient method for the simultaneous determination of RDX and HMX in rat plasma has been developed. This method provides a new approach for the analysis of nitramine compounds in biological samples.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Chi-Hang Chow, Kelvin Sze-Yin Leung
Article
Environmental Sciences
Yanran Huang, Japhet Cheuk-Fung Law, Yingya Zhao, Huijing Shi, Yunhui Zhang, Kelvin Sze-Yin Leung
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Environmental Sciences
Chi-Hang Chow, Kelvin Sze-Yin Leung
Article
Environmental Sciences
Yanran Huang, Pengpeng Wang, Japhet Cheuk-Fung Law, Yingya Zhao, Qian Wei, Yuhan Zhou, Yunhui Zhang, Huijing Shi, Kelvin Sze-Yin Leung
ENVIRONMENT INTERNATIONAL
(2020)
Article
Engineering, Environmental
Wai-Kit Ho, Japhet Cheuk-Fung Law, Tong Zhang, Kelvin Sze-Yin Leung
Article
Engineering, Environmental
Chi-Hang Chow, Japhet Cheuk-Fung Law, Kelvin Sze-Yin Leung
Summary: UV/NH2Cl is an emerging advanced oxidation process for micropollutant removal, generating reactive chlorine species (RCS) and hydroxyl radicals. Transformation products of ACE treated with UV/NH2Cl were found to be more toxic, attributed to the chlorinated transformation products and specifically halo-alcohols. The study provides insight into the role of reactive species and potential risks in using UV/NH2Cl for micropollutant removal in water treatment.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Environmental Sciences
Yanran Huang, Japhet Cheuk-Fung Law, Tsz-Ki Lam, Kelvin Sze-Yin Leung
Summary: Organic UV filters are compounds that absorb UV radiation with a highly conjugated structure, and their demand has been increasing, making them emerging contaminants. They pose potential threats to both the environment and human health, with characteristics of bioaccumulation and adverse effects.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Wai-Kit Ho, Kelvin Sze-Yin Leung
Summary: This study investigated the uptake and release of nanoceria by various polystyrene MPs, showing that in the presence of heavy metals, the sorption affinity of PSMPs for CeNPs was substantially enhanced. The formation of PSMP-HM-CeNP complexes was responsible for this enhanced affinity, highlighting the crucial role of MPs in the transfer of engineered nanoparticles in aquatic environments.
Article
Biochemical Research Methods
Yingyan Huang, Judy Tsz-Shan Lum, Kelvin Sze-Yin Leung
Summary: An integrated analytical approach using ICP-MS and ultrafiltration was developed to successfully separate and characterize ionic Ce and CeO2 NPs, with enzymatic pretreatment found to be more efficient than alkaline pretreatment. The properties/states of all Ce species were well preserved, and the recovery rates of both species were over 85%.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Japhet Cheuk-Fung Law, Yanran Huang, Chi-Hang Chow, Tsz-Ki Lam, Kelvin Sze-Yin Leung
Summary: The study investigated the transformation products of organic UV filters as model micropollutants, revealing that the resulting products may have increased water solubility, persistence, and mobility, with enhanced toxicity compared to the parent compounds.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Analytical
Ka -Him Chun, Judy Tsz-Shan Lum, Kelvin Sze -Yin Leung
Summary: The study utilized single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) to analyze single particles/cells, and developed a method to optimize quadrupole voltages for expanded analytical capabilities while minimizing spectral interference. This technique successfully achieved accurate analysis of various particles and biological cells despite severe interference, showing great potential for practical applications.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Yue Jiang, Didar Baimanov, Shan Jin, Japhet Cheuk-Fung Law, Pengcheng Zhao, Juanjuan Tang, Jian Peng, Liming Wang, Kelvin Sze-Yin Leung, Wenchao Sheng, Sijie Lin
Summary: In this study, a titanium oxide-based Fenton-like catalyst was constructed using exfoliated MXene as a template, and successfully degraded atrazine, an agricultural herbicide.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Environmental
Wai-Kit Ho, Japhet Cheuk-Fung Law, Jason Cho-Wai Lo, Isaac Kai-Xin Chng, Catherine Hong-Huan Hor, Kelvin Sze-Yin Leung
Summary: The high affinity of microplastics (MPs) for heavy metals has attracted attention. This study investigated the role of benzophenone-type UV filters on the sorption, oxidation state, and toxicity of chromium (Cr) by polystyrene MPs (PSMPs). Results showed that Cr uptake by PSMPs increased significantly with the presence of UV filters, leading to altered oxidation state and increased toxicity. This study provides evidence on the effects of coexisting constituents on the oxidation states and toxicities of MP-bound heavy metals.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Yingyan Huang, Judy Tsz-Shan Lum, Wai-Kit Ho, Kelvin Sze-Yin Leung
Summary: This study investigated the impact of engineered nanoparticles (ENPs) and heavy metals on human health using in vitro and mouse models. The results showed that ENPs decreased the bioaccessibility of heavy metals during the digestive process and increased their bioaccumulation in organs. The size of the nanoparticles and redox reactions on their surface could enhance the toxic effects. This study provides the first step in understanding the effects of ENPs and heavy metals on mammalian health in the environment.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Analytical
Yingyan Huang, Judy Tsz-Shan Lum, Kelvin Sze-Yin Leung
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2020)
