Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 23, Pages 7397-7404Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-7193-6
Keywords
Carnitine; Acetylcarnitine; Biological fluids; Hydrophilic interaction liquid chromatography-tandem mass spectrometry
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Agencia Nacional de Promocion Cientifica y Tecnologica
- Universidad Nacional de San Luis (INQUISAL)
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Monitoring carnitine and acetylcarnitine levels in biological fluids is a powerful tool for diagnostic studies. Research has recently shown that the analysis of carnitine and related compounds in clinical samples can be accomplished by different analytical approaches. Because of the polar and ionic nature of the analytes and matrix complexity, accurate quantitation is a highly challenging task. Thus, sample processing factors, preparation/cleanup procedures, and chromatographic/ionization/detection parameters were evaluated. On the basis of the results obtained, a rapid, selective, sensitive method based on hydrophilic interaction liquid chromatography-tandem mass spectrometry for the analysis of carnitine and acetylcarnitine in serum and urine samples is proposed. The matrix effect was assessed. The proposed approach was validated, the limits of detection were in the nanomolar range, and carnitine and acetylcarnitine levels were found within the micromolar range in both types of sample.
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