Review
Nutrition & Dietetics
Veronica Segura, Angela Ruiz-Carnicer, Carolina Sousa, Maria de Lourdes Moreno
Summary: The only current treatment for celiac disease is a strict gluten-free diet, but there is a need for non-dietary alternatives to prevent damage from dietary transgressions. Recent therapies for CD focus on degrading gluten, regulating the immune response, and inducing immunological tolerance, with promising results seen in some phase III and II clinical trials.
Review
Biochemistry & Molecular Biology
Charlene B. Van Buiten, Ryan J. Elias
Summary: Celiac disease is an autoimmune disorder characterized by heightened immune response to gluten proteins, with treatment involving complete avoidance of gluten in the diet. Ongoing clinical trials are targeting the immune response and gluten proteins, while recent studies suggest that polyphenols may offer protection by disrupting enzymatic hydrolysis of gluten and exerting anti-inflammatory effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jorma Isola, Markku Maeki, Martin Hils, Ralf Pasternack, Keijo Viiri, Valeriia Dotsenko, Toni Montonen, Timo Zimmermann, Ralf Mohrbacher, Roland Greinwald, Detlef Schuppan
Summary: In this study, the accumulation of ZED1227 after oral administration of the drug was studied. It was found that ZED1227 mainly accumulated in the villous enterocytes and had an inhibitory effect on TG2 activity. These findings are significant for the treatment of celiac disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Food Science & Technology
Kalekristos Yohannes Woldemariam, Juanli Yuan, Zhen Wan, Qinglin Yu, Yating Cao, Huijia Mao, Yingli Liu, Jing Wang, Hongyan Li, Baoguo Sun
Summary: Wheat mainly consists of gluten with 30% gliadin and 50% glutenin, which are associated with celiac disease. The challenge lies in discovering new gluten-based peptides with high affinity toward T-cells. The highly immunogenic 33-mer gliadin peptide is considered the main cause, so consistent data on different gluten peptides and their immunogenicity is urgently needed.
FOOD REVIEWS INTERNATIONAL
(2022)
Article
Chemistry, Applied
Darina Pronin, Andreas Boerner, Katharina Anne Scherf
Summary: Analysis of the quantities of four CD-active peptides in 60 German hexaploid winter wheat cultivars from 1891 to 2010 showed that breeding has not significantly altered the immunoreactive potential of wheat. The levels of CD-active peptides were more influenced by harvest year than by cultivar, indicating similar immunoreactive potential in old and modern wheat cultivars.
Article
Pharmacology & Pharmacy
Arathi Kulkarni, Shuchi Patel, Deepesh Khanna, Mayur S. Parmar
Summary: Celiac Disease (CeD) is an autoimmune disorder caused by the consumption of gluten, leading to inflammation in the small intestine. The current treatment recommendation is to avoid gluten-based products, as there is no FDA-approved medication for CeD. However, several clinical trials are underway to develop pharmacological approaches to treat CeD.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Review
Nutrition & Dietetics
Bara Aljada, Ahmed Zohni, Wael El-Matary
Summary: The gluten-free diet is popular for treating immune-mediated disorders caused by gluten, but it has disadvantages like high costs, nutritional deficiencies, and social and psychological barriers. Close follow-up with patients following the diet is recommended, and more data is needed to assess its effectiveness in managing mental and cognitive disorders.
Review
Chemistry, Multidisciplinary
Haixiang Yu, Obtin Alkhamis, Juan Canoura, Yingzhu Liu, Yi Xiao
Summary: This review discusses the challenges and advances in isolating and characterizing small-molecule-binding DNA aptamers, including in vitro methodologies for isolation, strategies for optimal binding properties, techniques for characterizing aptamer-target binding and structure, and various small-molecule sensing platforms and detection applications based on aptamers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Food Science & Technology
Xiaoxue Zhu, Xin-Huai Zhao, Qiang Zhang, Na Zhang, Olugbenga P. Soladoye, Rotimi E. Aluko, Yuhao Zhang, Yu Fu
Summary: This article reviews the latest research progress on the relationship between celiac disease (CD) and gluten. It emphasizes the structure and function of gluten peptides related to CD, gluten detection methods, the effects of processing on gluten, and gluten-free diets. The limitations in current CD research are also discussed. This study facilitates a comprehensive understanding of CD and gluten, providing a theoretical reference for future research.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Gastroenterology & Hepatology
Benjamin Lebwohl, Alberto Rubio-Tapia
Summary: The incidence of celiac disease is increasing due to both improved recognition and a real increase of the disorder. The reasons for this true rise are unclear, but may be related to environmental factors. Early-life gluten exposure is a major focus of prevention efforts.
Article
Biochemistry & Molecular Biology
Heinz Fabian Raber, Dennis Horst Kubiczek, Nicholas Bodenberger, Ann-Kathrin Kissmann, Deena D'souza, Hu Xing, Daniel Mayer, Pengfei Xu, Uwe Knippschild, Barbara Spellerberg, Tanja Weil, Frank Rosenau
Summary: This study highlights the development and characterization of DNA aptamers for fast labeling of human gut bacterium Akkermansia muciniphila and their potential impact on Alzheimer's disease. The results demonstrate the universal applicability of aptamers in modern microbiology techniques, including fluorimetry, confocal laser scanning microscopy, and flow cytometry. The findings suggest that these aptamers may have promising prospects for developing novel assays in clinical microbiology and electronic biosensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Nutrition & Dietetics
Fardowsa Abdi, Saania Zuberi, Jedid-Jah Blom, David Armstrong, Maria Ines Pinto-Sanchez
Summary: A gluten-free diet is essential for treating celiac disease and may improve symptoms in non-celiac gluten/wheat sensitivity. However, adopting a gluten-free diet or a gluten-restricted diet may increase the risk of malnutrition and nutrient deficiencies. Therefore, patients with these conditions should undergo nutritional assessment and monitoring, and receive multidisciplinary care for long-term nutrition management.
Review
Immunology
Renata Auricchio, Riccardo Troncone
Summary: Celiac disease is an autoimmune disorder triggered by gluten, with prevention strategies focusing on identifying at-risk individuals and eliminating risk factors. Research has shown that both genetic and environmental factors play a role in the development of the disease, suggesting that future interventions could involve protection from infections, manipulation of microbiota, and modulation of T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Amanda K. Cartee, Rok Seon Choung, Katherine S. King, Suyue Wang, John L. Dzuris, Robert P. Anderson, Carol T. Van Dyke, Chadrick A. Hinson, Eric Marietta, David A. Katzka, Vandana Nehra, Madhusudan Grover, Joseph A. Murray
Summary: Symptoms do not reliably indicate gluten exposure in patients with celiac disease or nonceliac gluten sensitivity. For patients with celiac disease, gluten can induce rapid-onset T-cell activation despite long-term treatment.
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Food Science & Technology
Silvia Grassi, Mariolina Gulli, Giovanna Visioli, Alessandra Marti
Summary: The study demonstrates that gluten aggregation properties can be a reliable indicator for differentiating the quality of durum wheat cultivars. Through Principal Component Analysis, it was found that gluten aggregation characteristics can effectively separate wheat varieties of different quality classes, making it a useful tool for assessing durum wheat semolina quality.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ciara K. O' Sullivan, Teresa Mairal, Miriam Jauset-Rubio, Marketa Svobodova, Vasso Skouridou, Veronica Esposito, Antonella Virgilio, Aldo Galeone
Summary: This study delved into the structural forms of an 11-mer aptamer and its impact on binding ability, revealing nanomolar affinity in both monomeric and higher-order structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemical Research Methods
Xhensila Shkembi, Marketa Svobodova, Vasso Skouridou, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Ciara K. O'Sullivan
Summary: This review discusses the selection of aptamers against mycotoxins and their incorporation into optical and electrochemical aptasensors. The strategies, advantages, and disadvantages of each approach are outlined, along with the different materials used and immobilization methods employed to achieve the highest sensitivity and selectivity.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ivan Magrina, Mayreli Ortiz, Anna Simonova, Michal Hocek, Ciara K. O'Sullivan, Robert J. Forster
Summary: The study involved modifying DNA with special chemicals for hybridization with a gold electrode surface probe to investigate the characteristics of potential and electron transfer rate. The results showed that the charge state of DNA and the concentration of Sr2+ ions significantly influenced the rate of electron transfer.
Article
Chemistry, Analytical
Sallam AL-Madhagi, Ciara K. O'Sullivan, Mamas I. Prodromidis, Ioanis Katakis
Summary: A reagent-less DNA sensor utilizing gold nanoparticles, modified primers, and isothermal amplification has been developed and successfully applied for the detection of Karlodinium armiger. The sensor demonstrates high sensitivity and rapid detection speed through optimized reaction conditions.
Article
Biotechnology & Applied Microbiology
Greta Gaiani, Anna Toldra, Karl B. Andree, Maria Rey, Jorge Diogene, Carles Alcaraz, Ciara K. O'Sullivan, Monica Campas
Summary: The study developed three molecular assays for detecting Gambierdiscus and Fukuyoa genera, as well as G. australes and G. excentricus species, achieving a remarkable limit of detection of a single cell. These assays provide a rapid and cost-effective strategy for reliable screening of samples and ciguatera risk assessment, ensuring seafood safety and protection of human health.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
David Kodr, Cansu Pinar Yenice, Anna Simonova, Dijana Pavlovic Saftic, Radek Pohl, Veronika Sykorova, Mayreli Ortiz, Ludek Havran, Miroslav Fojta, Zbigniew J. Lesnikowski, Ciara K. O'Sullivan, Michal Hocek
Summary: A series of modified nucleoside triphosphates bearing dicarba-nido-undecaborate or metal bis(dicarbollide) groups were prepared and utilized for DNA synthesis and redox labeling. The combination of these redox labels allowed for the first fully orthogonal redox coding of all four canonical nucleobases, enabling determination of nucleobase composition of short DNA stretches with electrochemical readout in one simple primer extension experiment.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Miriam Jauset-Rubio, Mayreli Ortiz, Ciara K. O'Sullivan
Summary: Isothermal recombinase polymerase amplification-based solid-phase primer extension is utilized for the optical detection of a single nucleotide polymorphism related to hypertrophic cardiomyopathy in fingerprick blood samples. By optimizing the percentage of biotinylated dNTPs and the duration of primer extension, the SNP in the fingerprick sample was identified successfully. Ongoing work is focused on expanding the technology to the multiplexed detection of SNPs in fingerprick and other biological samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xhensila Shkembi, Vasso Skouridou, Marketa Svobodova, Sandra Leonardo, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Monica Campas, Ciara K. O'Sullivan
Summary: This study developed an aptamer-based detection method for the rapid and sensitive detection of TTX in pufferfish, using next-generation sequencing technology. The results demonstrated that the developed hybrid antibody-aptamer sandwich assay outperformed several methods reported in the literature and commercial immunoassay kits.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Mayreli Ortiz, Miriam Jauset-Rubio, David Kodr, Anna Simonova, Michal Hocek, Ciara K. O'Sullivan
Summary: This study developed an electrochemical platform for the detection of SNP in the MYH7 gene associated with hypertrophic cardiomyopathies using fingerprick blood samples. The platform uses solid-phase primer elongation and recombinase polymerase amplification to accurately identify SNPs, and only requires a small amount of fingerprick blood for analysis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Environmental Sciences
Greta Gaiani, Francesca Cucchi, Anna Toldra, Karl B. Andree, Maria Rey, Takeshi Tsumuraya, Ciara K. O'Sullivan, Jorge Diogene, Monica Campas
Summary: Several genera of marine dinoflagellates are capable of producing bioactive compounds that can impact human health, with Gambierdiscus and Fukuyoa standing out for their production of toxins, including ciguatoxins. A multiplex detection system and rapid DNA extraction technique have been developed for identifying toxin-producing species, enabling swift detection in field samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Xhensila Shkembi, Mary Luz Botero, Vasso Skouridou, Miriam Jauset-Rubio, Marketa Svobodova, Pablo Ballester, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Abdulrahman O. Alyoubi, Ciara K. O. Sullivan
Summary: The illicit use of AAS as performance-enhancing drugs poses a threat to both competitive sports and public health. Current detection methods are limited to laboratory settings and cannot be performed on-site. However, the development of aptamer technology offers the potential for simple and cost-effective on-site analysis. In this study, a label-free colorimetric assay was developed using aptamers to detect the abuse of AAS.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Koena L. Moabelo, Teresa M. Lerga, Miriam Jauset-Rubio, Nicole R. S. Sibuyi, Ciara K. O'Sullivan, Mervin Meyer, Abram M. Madiehe
Summary: This study presents the development of a rapid aptasensor based on gold nanoparticles for the detection of RBP4. The method utilizes the specific binding of aptamers to RBP4 and the color change of gold nanoparticles to achieve rapid detection, with results available within 5 minutes.
Article
Chemistry, Analytical
Miriam Jauset-Rubio, Mayreli Ortiz, Ciara K. O'Sullivan
Summary: Aptamer-based assays and sensors show promise as alternatives to antibodies for their flexibility in assay formats. This study demonstrates the use of a biotin-modified aptamer in enhancing sensitivity and achieving lower detection limits in a colorimetric enzyme-linked oligonucleotide assay and electrochemical detection. The generic nature of this assay format allows for easy and cost-effective application to various aptamers.
Article
Chemistry, Analytical
Daniel Torregrosa, Miriam Jauset-Rubio, Raquel Serrano, Marketa Svobodov, Guillermo Grindlay, Ciara K. O'Sullivan, Juan Mora
Summary: The objective of this study is to evaluate different competitive aptamer assays for the detection of beta-conglutin in flour samples using inductively coupled plasma mass spectrometry (ICP-MS). Two competitive aptamer assay schemes were developed, one using thiolated aptamers on gold nanoparticles and the other using biotinylated aptamers linked to streptavidin-AuNPs. The influence of ICP-MS detection mode on assay performance was explored. The optimized condition achieved a LOD of 2 pM, significantly improving the LOD compared to previous studies.
ANALYTICA CHIMICA ACTA
(2023)