Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 404, Issue 6-7, Pages 1771-1778Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6296-9
Keywords
Bioanalytical methods; Biological samples; Drug monitoring; Drug screening; IR spectroscopy; Raman spectroscopy
Funding
- Eurasia-Pacific Uninet (Salzburg, Austria)
- Ministry for Science and Research
- Ministry for Health, Family and Youth (Vienna, Austria) [80855]
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In the present study, Fourier transform infrared (FTIR) imaging and data analysis methods were combined to study morphological and molecular patterns of St. John's wort (Hypericum perforatum) in detail. For interpretation, FTIR imaging results were correlated with histological information gained from light microscopy (LM). Additionally, we tested several evaluation processes and optimized the methodology for use of complex FTIR microscopic images to monitor molecular patterns. It is demonstrated that the combination of the used spectroscopic method with LM enables a more distinct picture, concerning morphology and distribution of active ingredients, to be gained. We were able to obtain high-quality FTIR microscopic imaging results and to distinguish different tissue types with their chemical ingredients.
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