Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 402, Issue 10, Pages 3205-3209Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5667-y
Keywords
PTK7; sgc8; Aptamer; Antibody; Rupture force; Atomic force microscopy
Funding
- National Basic Research Program of China [2011CB911001]
- NSFC [20121063]
- NSF EAPSI
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Some researchers have questioned whether synthetic aptamers bind as robustly as natural antibodies. To address this issue, we used single-molecule atomic force microscopy to measure the rupture force between a protein and both its aptamer and its antibody. The rupture force on live cell membranes between the aptamer and protein was 46 +/- 26 pN; the force with the antibody was 68 +/- 33 pN, we conclude that the binding forces are about equal.
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