Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 400, Issue 9, Pages 2755-2761Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-4811-z
Keywords
SERS; Microfluidic lab-on-a-chip; Enzyme activity; Thiopurine methyltransferase; 6-Mercaptopurine
Funding
- Free State of Thuringia
- European Union (EFRE) [2008FE9112, 2008FE9113 (BioOptiSens)]
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In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.
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