Article
Food Science & Technology
B. S. Gnanesh Kumar, Simran Mattad
Summary: The study investigated the glycan diversity of bLf in colostrum, revealing significant differences in glycan heterogeneity and relative abundance at each site. Analysis showed that bLf at different sites predominantly contain various types of glycans, with potential presence of additional protein lipocalin-2 in commercial preparations.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Chemistry, Analytical
Xueting Jin, Canhong Zhu, Jiani Wu, Yinghua Yan, Chuan-Fan Ding, Keqi Tang, Di Zhang
Summary: By modifying a magnetic graphene material, a hydrophilic nanocomposite material with good enrichment effect and stability was successfully prepared. It exhibits high selectivity and sensitivity for glycopeptide enrichment, showing potential for application in enriching N-glycopeptides from complex biological samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Oncology
Jun Li, Ting Zhao, Jing Li, Jiechen Shen, Li Jia, Bojing Zhu, Liuyi Dang, Chen Ma, Didi Liu, Fan Mu, Liangshuo Hu, Shisheng Sun
Summary: This study identified numerous unique N-glycan structures in ICC through precision N-glycoproteomic analysis, providing a valuable resource for future research on ICC diagnostic biomarkers, therapeutic targets, and mechanism investigations.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemical Research Methods
H. Mehmet Kayili, Bekir Salih
Summary: This study utilized high-throughput glyco-analytical strategies to analyze the N-glycosylation sites and glycan contents of human thyroglobulin. The results revealed the presence of high-mannose type glycans at some sites and complex/hybrid type glycans at other regions, with sulfated glycoform structures detected at sites containing complex/hybrid type glycans.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemical Research Methods
Concepcion A. Remoroza, Meghan C. Burke, Xiaoyu Yang, Sergey Sheetlin, Yuri Mirokhin, Sanford P. Markey, Dmitrii V. Tchekhovskoi, Stephen E. Stein
Summary: A mass spectral library-based method for analyzing site-specific N-linked protein glycosylation is presented. It is applied to human milk glycoproteins data to demonstrate its operation and utility. Two types of mass spectral libraries are generated, one containing glycopeptide abundance distribution spectra and the other containing tandem mass spectra of the underlying glycopeptides.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Biochemical Research Methods
Jason C. Maynard, Robert J. Chalkley
Summary: O-GlcNAcylation, a widespread regulatory posttranslational modification, plays a role in response to nutritional status and stress, with dysregulation linked to various diseases. Advancements in enrichment and mass spectrometry techniques have accelerated research into this modification in recent years.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Cell Biology
Piaopiao Wen, Jingru Chen, Chenyang Zuo, Xiaodong Gao, Morihisa Fujita, Ganglong Yang
Summary: STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex, playing different roles in N-linked glycosylation. Deletion of the STT3A gene has a significant impact on protein expression, particularly on glycoproteins. This deletion also affects the expression of glycan-related enzymes, resulting in altered levels of mannosylated and fucosylated N-glycans. Hyperglycosylated proteins are identified in STT3A-KO cells, which are associated with endoplasmic reticulum stress and unfolded protein response.
Article
Biochemistry & Molecular Biology
Mary Rachel Nalehua, Joseph Zaia
Summary: The biosynthetic enzymes in the secretory pathway create distributions of glycans at each glycosite that influence the biophysical properties and biological functions of glycoproteins. Understanding the heterogeneity of protein glycosylation is essential for quantifying changes in biological processes. Recent technical advances in mass spectral glycoproteomics analysis have improved our ability to accurately quantify these glycosylation changes.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Janelle Sauvageau, Izel Koyuturk, Frank St. Michael, Denis Brochu, Marie-France Goneau, Ian Schoenhofen, Sylvie Perret, Alexandra Star, Anna Robotham, Arsalan Haqqani, John Kelly, Michel Gilbert, Yves Durocher
Summary: Glycosylation is an important quality attribute for protein therapeutics, but current methods for monitoring glycosylation are time-consuming and difficult to analyze. In this study, researchers evaluated the use of HPAEC-PAD analysis to quickly and reliably detect glycosylation differences between protein production batches. The results were consistent with those obtained by HILIC-Fld and LC-MS.
COMMUNICATIONS CHEMISTRY
(2023)
Article
Biochemical Research Methods
Wenbo Dong, Lin Chen, Li Jia, Zexuan Chen, Jiechen Shen, Pengfei Li, Shisheng Sun
Summary: The low abundance and heterogeneity of N-glycosylation at the peptide level poses a great challenge in the field of glycobiology. In this study, different combinations of desalting and enrichment columns were compared for the enrichment of intact N-glycopeptides from mouse brain tissues. Results showed that the C18 column was slightly superior to the HLB column, and the use of both MAX and HILIC columns complemented each other in the enrichment of intact N-glycopeptides. Based on these findings, sequential enrichment of glycopeptides using HILIC and then MAX columns is recommended for in-depth analysis of site-specific glycoproteome.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Soumya Mukherjee, Andris Jankevics, Florian Busch, Markus Lubeck, Yang Zou, Gary Kruppa, Albert J. R. Heck, Richard A. Scheltema, Karli R. Reiding
Summary: Ion mobility enables spatial separation of ions in the gas phase, providing information about their size. The timsTOF Pro device can physically separate N-glycopeptides from nonmodified peptides and produce high-quality fragmentation spectra. This method allows for the effective selection of analytes of interest based on the clear cluster in the mobiologram formed by the glycan moieties enlarging the size of glycopeptides.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Immunology
Iwona Wojcik, Manfred Wuhrer, Peter Heeringa, Coen A. Stegeman, Abraham Rutgers, David Falck
Summary: This study found that the glycosylation changes of IgG are associated with disease flares in patients with ANCA-associated vasculitis. In addition, among the glycosylation changes, alterations in Fc bisection and fucosylation levels of IgG are correlated with long-term treatment outcome and impending relapse.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Haoru Zhuang, Huiran Hao, Yongshun Qiu, Jinjuan Gan, Tianlu Li
Summary: Protein glycosylation is the most complex and diverse form of post-translational modification in the human body. Finding ways to improve the pharmacokinetic profile of peptide- and protein-based therapeutics, glycosylation of peptides and proteins has emerged as a promising strategy. Different synthesis strategies have attracted attention in the preparation of structurally defined glycopeptides and glycoproteins, enabling a thorough investigation of the specific effects of protein glycosylation. This review highlights recent progress in the development of novel strategies, preparation of homogeneous glycoproteins, and exploration of structure-activity relationships, while also discussing challenges and prospects.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mengyao Fu, Baichun Wang, Linhua Yi, Xueting Jin, Yinghua Yan, Chuan-Fan Ding
Summary: A novel porous bifunctionalized composite material with superhydrophilicity and small steric hindrance was synthesized via a simple method, showing excellent performance in glycopeptides enrichment.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Zexuan Chen, Jiechen Shen, Wenbo Dong, Pengfei Li, Miaomiao Xin, Didi Liu, Li Jia, Bojing Zhu, Wenzhe Li, Shisheng Sun
Summary: This method presents a simple and direct approach for recognizing core-fucosylated glycopeptides based on the relative intensities of Y1+Fuc ions, showing potential for manual interpretation and software-based high-throughput analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Ventzislava Hristova, Shisheng Sun, Hui Zhang, Daniel W. Chan
CLINICAL PROTEOMICS
(2020)
Article
Chemistry, Analytical
Xiaolu Li, Qian Feng, Lejing Qu, Ting Zhao, Xiaoan Li, Tiantian Bai, Shisheng Sun, Shaoping Wu, Yongmin Zhang, Jianli Li
ANALYTICA CHIMICA ACTA
(2020)
Review
Biochemical Research Methods
Rongxia Lan, Miaomiao Xin, Zhifang Hao, Shanshan You, Yintai Xu, Jingyu Wu, Liuyi Dang, Xinwen Zhang, Shisheng Sun
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Multidisciplinary Sciences
Jianbo Pan, Yingwei Hu, Shisheng Sun, Lijun Chen, Michael Schnaubelt, David Clark, Minghui Ao, Zhen Zhang, Daniel Chan, Jiang Qian, Hui Zhang
NATURE COMMUNICATIONS
(2020)
Article
Biology
Pengfei Li, Zhifang Hao, Huanhuan Liu, Bojing Zhu, Liuyi Dang, Chen Ma, Yintai Xu, Yiyan Zhang, Daidi Fan, Shisheng Sun
Summary: Colon cancer is a highly lethal malignancy worldwide, and berberine has shown potential anti-colon cancer activity both in vitro and in vivo. Through proteomics, this study identified key proteins and regulatory networks associated with berberine treatment of colon cancer, shedding light on potential therapeutic targets for colon cancer.
Article
Biochemical Research Methods
Jiechen Shen, Li Jia, Liuyi Dang, Yuanjie Su, Jie Zhang, Yintai Xu, Bojing Zhu, Zexuan Chen, Jingyu Wu, Rongxia Lan, Zhifang Hao, Chen Ma, Ting Zhao, Ni Gao, Jieyun Bai, Yuan Zhi, Jun Li, Junying Zhang, Shisheng Sun
Summary: This study introduces a modularization strategy and an algorithm named StrucGP for automated interpretation of N-glycan structures using tandem mass spectrometry, enabling detailed glycan structure determination on thousands of glycosites. The approach aids in identifying rare/new glycan structures and facilitates in-depth structural and functional study of glycoproteins in the biomedical research field. StrucGP offers a novel de novo glycan mapping method for determining detailed N-glycan structures at the site-specific level.
Article
Immunology
Pengfei Li, Zhifang Hao, Jingyu Wu, Chen Ma, Yintai Xu, Jun Li, Rongxia Lan, Bojing Zhu, Pengyu Ren, Daidi Fan, Shisheng Sun
Summary: This study systematically investigated the protein expression profiles of M1 and M2 phenotypes in human THP-1 and mouse RAW264.7 macrophages, revealing significant divergences between the two cell lines while also identifying some common up-regulated proteins in M1 macrophages. These data serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Miaomiao Xin, Shanshan You, Yintai Xu, Wenhao Shi, Bojing Zhu, Jiechen Shen, Jingyu Wu, Cheng Li, Zexuan Chen, Yuanjie Su, Juanzi Shi, Shisheng Sun
Summary: This study identifies distinctive glycoproteins in spermatozoa that are involved in various important processes, such as spermatogenesis, acrosome reaction, sperm-egg recognition, and fertilization. The research also highlights the different glycosylation patterns in spermatozoa compared to other tissues. These findings provide valuable insights into the glycosylation of spermatozoa and lay the foundation for further studies on male infertility.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Oncology
Jun Li, Ting Zhao, Jing Li, Jiechen Shen, Li Jia, Bojing Zhu, Liuyi Dang, Chen Ma, Didi Liu, Fan Mu, Liangshuo Hu, Shisheng Sun
Summary: This study identified numerous unique N-glycan structures in ICC through precision N-glycoproteomic analysis, providing a valuable resource for future research on ICC diagnostic biomarkers, therapeutic targets, and mechanism investigations.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemical Research Methods
Yuan Zhi, Li Jia, Jiechen Shen, Jun Li, Zexuan Chen, Bojing Zhu, Zhifang Hao, Yintai Xu, Shisheng Sun
Summary: Formic acid solution is commonly used for dissolving glycopeptide and glycan samples before mass spectrometry analysis. However, storing the samples in formic acid solution at -20°C can lead to an undesirable modification that affects the identification and quantification of glycans. Therefore, storing samples at -20°C should be avoided.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Miaomiao Xin, Yintai Xu, Shanshan You, Cheng Li, Bojing Zhu, Jiechen Shen, Zexuan Chen, Wenhao Shi, Xia Xue, Juanzi Shi, Shisheng Sun
Summary: In this study, a precise site-specific N-glycoproteome map of human seminal plasma was established using a site-specific glycoproteomic approach and a glycan structure interpretation software. The results revealed a diverse range of glycoproteins in seminal plasma, which is crucial for studying the glycosylation structure and function in the male reproductive system.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Pengfei Li, Chen Ma, Jing Li, Shanshan You, Liuyi Dang, Jingyu Wu, Zhifang Hao, Jun Li, Yuan Zhi, Lin Chen, Shisheng Sun
Summary: In this study, the protein expression profiles of human THP-1-derived macrophages (M0, M1, M2a, M2b, M2c, and M2d) were systematically analyzed using quantitative proteomics approaches. The results showed that M2a and M2b, and M2c and M2d have very similar protein expression profiles. These findings are important for the study of macrophages using THP-1 cells and for distinguishing different M2 subtypes in macrophage-associated diseases.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2022)
Article
Medicine, Research & Experimental
Li Jia, Jun Li, Pengfei Li, Didi Liu, Jing Li, Jiechen Shen, Bojing Zhu, Chen Ma, Ting Zhao, Rongxia Lan, Liuyi Dang, Wang Li, Shisheng Sun
Summary: The study identified core-fucosylation as a key glycosylation modification linked to the promotion of EMT in HCC cells, with FOLR1 as a potential target for therapeutic interventions. The up-regulation of FUT8 was found to drive EMT induced by HGF/TGF-beta 1, highlighting the importance of glycosylation in cancer progression. Further experiments demonstrated that core-fucosylation on FOLR1 enhanced folate uptake, ultimately promoting EMT in HCC cells.
Article
Biotechnology & Applied Microbiology
Joseph L. Mertz, Shisheng Sun, Bojiao Yin, Yingwei Hu, Rahul Bhattacharya, Michael J. Bettenbaugh, Kevin J. Yarema, Hui Zhang
BIOENGINEERING-BASEL
(2020)
Article
Biochemistry & Molecular Biology
Xilong Wang, Tianran Ma, Hanjie Yu, Zhuo Chen, Bojing Zhu, Wentian Chen, Shisheng Sun, Zheng Li
