Review
Chemistry, Multidisciplinary
Zhaowei Zhang, Peng Ma, Rajib Ahmed, Jie Wang, Demir Akin, Fernando Soto, Bi-Feng Liu, Peiwu Li, Utkan Demirci
Summary: The passage highlights the global threats caused by human acute respiratory virus infections and the importance of new technologies in addressing the inadequacies of traditional detection methods. It emphasizes the key elements in clinical sample testing and the significance of sensitivity, specificity, and speed in detecting respiratory viruses.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Shanyong Zhou, Datao Tu, Yan Liu, Wenwu You, Yunqin Zhang, Wei Zheng, Xueyuan Chen
Summary: This study proposes a facile and ultrasensitive detection method for tumor markers in saliva using luminescence-amplification strategy of lanthanide nanoprobes. By integrating dissolution enhanced luminescent bioassay strategy and miniaturized detection device, the unique lab-in-syringe assay enables the detection of tumor markers with high sensitivity and specificity, facilitating clinical diagnosis and home self-monitoring.
Article
Neurosciences
Andrea Cruz, Maria Vieira, Ana R. R. Mesquita, Adriana Sampaio, Ines Mendes-Pinto, Isabel Soares, Paulo P. P. Freitas
Summary: Inflammation is associated with mental health problems, and IL-6 levels in children can indicate psychological issues. This study developed an immunosensor that detected IL-6 in saliva samples from children, demonstrating improved speed and sensitivity compared to conventional ELISA. The point-of-care immunosensor has potential as a non-invasive, high-sensitive monitoring tool for mental health problems in vulnerable populations like children.
FRONTIERS IN NEUROSCIENCE
(2022)
Review
Medicine, Research & Experimental
Trey W. Pittman, Daniel Balazs Decsi, Chamindie Punyadeera, Charles S. Henry
Summary: There has been a growing interest in using microfluidic devices for saliva analysis in recent years. This approach has the potential to provide more sensitive and selective analysis by collecting large quantities of saliva samples. The aim of this review is to provide an update on the use of microfluidic devices for salivary biomarker analysis.
Article
Multidisciplinary Sciences
Jerome LeGoff, Solen Kerneis, Caroline Elie, Severine Mercier-Delarue, Nabil Gastli, Laure Choupeaux, Jacques Fourgeaud, Marie-Laure Alby, Pierre Quentin, Juliette Pavie, Patricia Brazille, Marie Laure Nere, Marine Minier, Audrey Gabassi, Chrystel Leroy, Beatrice Parfait, Jean-Marc Treluyer, Constance Delaugerre
Summary: The study found that the sensitivity of detecting SARS-CoV-2 RNA in saliva using RT-LAMP is lower compared to nasopharyngeal RT-PCR, saliva RT-PCR, and nasopharyngeal antigen testing. Additionally, the sensitivity of saliva RT-LAMP testing varies significantly based on different reference test criteria.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Tania Pomili, Paolo Donati, Pier Paolo Pompa
Summary: The study presents a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The device features a unique design and simple fabrication process, with particles exhibiting visible color changes at non-physiological concentrations.
Article
Geriatrics & Gerontology
Neng Mira Atjo, Gita Vita Soraya, Rosdiana Natzir, Hasyim Kasyim, Haerani Rasyid, Gursharan Chana, Michael Erlichster, Efstratios Skafidas, Marhaen Hardjo
Summary: This study investigated the diagnostic accuracy of salivary osmolarity measurement for detecting dehydration in hypertensive adults with and without diuretic pharmacotherapy. The results showed that hypertensive patients on diuretics had higher urine specific gravity and salivary osmolarity values, indicating a higher risk of dehydration. Salivary osmolarity assessment correlated strongly with urine specific gravity assessment.
JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION
(2022)
Article
Virology
Rasmus Strand, Louise Thelaus, Nils Fernstrom, Torgny Sunnerhagen, Ylva Lindroth, Adam Linder, Magnus Rasmussen
Summary: The study evaluated three point-of-care antibody tests for detecting IgG and IgM against SARS-CoV-2, with similar performance in sensitivity for IgG but lower sensitivity for IgM. The tests showed potential for accurate use in routine clinical practice.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Health Care Sciences & Services
Francis L. Martin, Camilo L. M. Morais, Andrew W. Dickinson, Tarek Saba, Thomas Bongers, Maneesh N. Singh, Danielle Bury
Summary: Saliva can be used as a liquid biopsy for screening lung cancer and prostate cancer. By analyzing the bioconstituents in saliva samples using infrared spectroscopy and principal component analysis, a highly accurate model can be built for the detection of these cancers in primary care settings.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Chemistry, Analytical
Chaewon Jung, Mingon Kim
Summary: In this study, a modified paper-based lateral flow immunoassay (LFIA) called gap-LFIA was developed for the direct detection of saliva collected using cotton swabs. The method reduced interference and improved signals, allowing for the direct use of whole saliva without additional steps. Compared to traditional methods, the gap-LFIA showed less deviation in results and enabled clearer distinction between smokers and non-smokers.
Article
Chemistry, Analytical
Yang Bai, Qiuquan Guo, Junfeng Xiao, Mingyue Zheng, Dongxing Zhang, Jun Yang
Summary: The study introduces an inkjet-printed electrochemical enzyme-based biosensor system that works in conjunction with a smartphone for point-of-care analyte detection. The system demonstrates a wide linear range and rapid response time, making it suitable for saliva testing with high selectivity and stability. The proposed biosensor system shows promise for various analyte detections and can be easily operated without specific training, showcasing its potential for point-of-care applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Parasitology
R. Noordin, E. Osman, N. Kalantari, NS. Anuar, T. Gorgani-Firouzjaee, P. Sithithaworn, NM. Juri, A. Rahumatullah
Summary: Strongyloides stercoralis is a parasite that causes strongyloidiasis worldwide. A laboratory-based evaluation was conducted to assess the diagnostic sensitivity and specificity of a rapid point-of-care test for Strongyloides. The test showed high sensitivity and specificity, indicating its potential as a useful tool for patient diagnosis and detection in resource-limited settings.
Review
Chemistry, Analytical
Miroslav Pohanka
Summary: C-reactive protein is an important component of the immune system's response to pathological impulses and can be used for diagnosing various pathological states. The current methods for CRP diagnosis have limitations, but research on biosensors and bioassay devices is providing more sensitive and suitable tools.
Article
Microbiology
Madoka Kuramitsu, Haruka Momose, Yuichiro Uchida, Kenji Ishitsuka, Ryuji Kubota, Masahito Tokunaga, Atae Utsunomiya, Kunihiko Umekita, Yuuki Hashikura, Kisato Nosaka, Ki-Ryang Koh, Hitomi Nakamura, Yasuko Sagara, Rieko Sobata, Masahiro Satake, Koh Nagata, Yuri Hasegawa, Daisuke Sasaki, Hiroo Hasegawa, Tomoo Sato, Yoshihisa Yamano, Kou Hiraga, Kenta Tezuka, Emi Ikebe, Sahoko Matsuoka, Kazu Okuma, Toshiki Watanabe, Kiyonori Miura, Isao Hamaguchi
Summary: Espline HTLV-I/II is a rapid immunochromatographic antibody test with sufficient sensitivity and specificity for HTLV-1 screening, as confirmed through comparison with other existing tests.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Stephen Young, Stephanie N. Taylor, Catherine L. Cammarata, Katey G. Varnado, Celine Roger-Dalbert, Amanda Montano, Christen Griego-Fullbright, Cameron Burgard, Catherine Fernandez, Karen Eckert, Jeffrey C. Andrews, Huimiao Ren, Joseph Allen, Ronald Ackerman, Charles K. Cooper
Summary: The BD Veritor System for Rapid Detection of SARS-CoV-2 nucleocapsid antigen showed varied performance in different DSO ranges compared to PCR testing, with PPA values below 100%. However, Veritor demonstrated high agreement with Sofia 2 in detecting SARS-CoV-2.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Letter
Medical Laboratory Technology
Nick Verougstraete, Alain G. Verstraete, Veronique Stove, Matthijs Oyaert
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Medicine, General & Internal
Veerle Casneuf, Ivan Borbath, Marc van den Eynde, Yolanda Verheezen, Wim Demey, Alain G. Verstraete, Kathleen Bm Claes, Vincent Haufroid, Karen P. Geboes
Summary: Fluoropyrimidines are commonly used in the treatment of malignancies, but genetic variations within the DPYD gene can lead to reduced DPD activity, causing potential toxicity. In Belgium, screening for DPD deficiency before starting treatment with fluoropyrimidines is recommended, with a preference for phenotype testing due to higher sensitivity and lower cost to society.
ACTA CLINICA BELGICA
(2022)
Article
Medicine, General & Internal
Viviane Van Hoof, Dragos Barglazan, Laurent Blairon, Bob Braekevelt, Regis Debois, Nathalie Veronique J. De Vos, Damien Gruson, Jef Jonckheere, Katrien Lanckmans, Marc Moens, Bart Peeters, Joris Penders, Alain Roman, Lieve Van Hoovels, Florent Vanstapel, Jan Y. Verbakel, Ann Verdonck, Alain G. Verstraete
Summary: There is a lack of legal framework for the use of POCT outside hospitals. It is proposed that tests should be carried out within a legal framework and in collaboration with clinical laboratories to ensure quality and accuracy. Government investment can be offset by health benefits.
ACTA CLINICA BELGICA
(2022)
Article
Biochemical Research Methods
Sarah Van Vooren, Alain G. Verstraete
Summary: An UHPLC-HRMS method was developed for simultaneous and sensitive quantification of 10 beta-lactam antibiotics, linezolid, and beta-lactamase inhibitors in human plasma. The method showed high sensitivity and accuracy, allowing it to be suitable for routine therapeutic drug monitoring.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Clinical Neurology
Marie-Gabrielle Goossens, Paul Boon, Wytse Wadman, Chris Van den Haute, Veerle Baekelandt, Alain G. Verstraete, Kristl Vonck, Lars E. Larsen, Mathieu Sprengers, Evelien Carrette, Jana Desloovere, Alfred Meurs, Jean Delbeke, Christian Vanhove, Robrecht Raedt
Summary: This study demonstrates potent seizure-suppressing effects in the intraperitoneal kainic acid rat model by chemogenetically inhibiting the hippocampus, even 1 year after viral transduction. Despite the need for further optimization, chemogenetic neuromodulation represents a promising treatment prospect for temporal lobe epilepsy.
Article
Medical Laboratory Technology
Nick Verougstraete, Veronique Stove, Alain G. Verstraete, Matthijs Oyaert
Summary: This study evaluated the performance of a new nanoparticle-based competitive immunoassay on two routine clinical chemistry analyzers and compared it to high-resolution mass spectrometry. The results showed that the immunoassay had good analytical performance and high agreement with the mass spectrometry results.
THERAPEUTIC DRUG MONITORING
(2022)
Article
Toxicology
Marthe M. Vandeputte, Nick Verougstraete, Donna Walther, Grant C. Glatfelter, Jeroen Malfliet, Michael H. Baumann, Alain G. Verstraete, Christophe P. Stove
Summary: This study provides a detailed chemical analysis and pharmacological characterization of N-Piperidinyl etonitazene. The results indicate that N-Piperidinyl etonitazene is a potent opioid with the potential to cause harm in users.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Critical Care Medicine
Tatjana Van der Heggen, Evelyn Dhont, Jef Willems, Ingrid Herck, Joris R. Delanghe, Veronique Stove, Alain G. Verstraete, Sophie Vanhaesebrouck, Peter De Paepe, Pieter A. J. G. De Cock
Summary: This study aimed to investigate the target attainment of beta-lactam antibiotics in critically ill children, identify risk factors for suboptimal exposure, and examine the association between target nonattainment and clinical outcome.
PEDIATRIC CRITICAL CARE MEDICINE
(2022)
Review
Oncology
Nick Verougstraete, Veronique Stove, Alain G. Verstraete, Christophe P. Stove
Summary: Therapeutic drug monitoring (TDM) of tyrosine kinase inhibitors (TKIs) is not yet performed routinely in oncology patients, but it has the potential to improve treatment outcomes and minimize toxicity. This review focuses on the use of dried blood microsamples for quantifying TKIs, discussing various methodological aspects and challenges associated with quantitative analyses. The use of dried blood microsamples offers convenience, cost-effectiveness, and improved compound stability.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medical Laboratory Technology
Wim Coucke, Corinne Charlier, Kathleen Croes, Boris Mahieu, Hugo Neels, Christophe Stove, Jan Tytgat, Andre Vanescote, Alain G. Verstraete, Sarah Wille, Arnaud Capron
Summary: This study evaluates the performance of automated systems in ethanol concentration analysis. The results show that Alinity c performs poorly in terms of bias and measurement uncertainty, while Architect and Cobas c501/c502 systems have the lowest variability.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Medical Informatics
Jarne Verhaeghe, Sofie A. M. Dhaese, Thomas De Corte, David Vander Mijnsbrugge, Heleen Aardema, Jan G. Zijlstra, Alain G. Verstraete, Veronique Stove, Pieter Colin, Femke Ongenae, Jan J. De Waele, Sofie Van Hoecke
Summary: This study developed machine learning models for predicting piperacillin plasma concentrations and providing uncertainty quantification. The results showed that these models can accurately estimate piperacillin concentrations and provide useful uncertainty predictions when the same dosing regimens are used. However, their generalization capabilities to other dosing schemes are limited.
BMC MEDICAL INFORMATICS AND DECISION MAKING
(2022)
Article
Biochemical Research Methods
Nick Verougstraete, Annick Verhaeghe, Jan Germonpre, Herve Lebbinck, Alain G. Verstraete
Summary: Etazene is a novel synthetic opioid that has been detected through analysis of urine samples. Its metabolites can be used to determine exposure to Etazene.
DRUG TESTING AND ANALYSIS
(2023)
Letter
Infectious Diseases
Niklas Koehler, Soenke Andres, Matthias Merker, Viola Dreyer, Agnieszka John, Martin Kuhns, David Krieger, Eva Choong, Nick Verougstraete, Pia Abel zur Wiesch, Sebastian G. Wicha, Christina Koenig, Barbara Kalsdorf, Patricia M. Sanchez Carballo, Dagmar Schaub, Jim Werngren, Thomas Schon, Charles A. Peloquin, Nicolas Schoenfeld, Alain G. Verstraete, Laurent A. Decosterd, Rob Aarnoutse, Stefan Niemann, Florian P. Maurer, Christoph Lange
JOURNAL OF INFECTION
(2023)
Article
Critical Care Medicine
Thomas De Corte, Jarne Verhaeghe, Sofie Dhaese, Sarah Van Vooren, Jerina Boelens, Alain G. Verstraete, Veronique Stove, Femke Ongenae, Liesbet De Bus, Pieter Depuydt, Sofie Van Hoecke, Jan J. De Waele
Summary: This study evaluated the pathogen-based clinically relevant target attainment of piperacillin-tazobactam and meropenem in surgical ICU patients. Despite achieving better results in the pathogen-based scenario compared to the worst-case scenario, a substantial proportion of samples did not attain commonly used PK/PD targets.
ANNALS OF INTENSIVE CARE
(2023)
Meeting Abstract
Respiratory System
Niklas Koehler, Rob Aarnoutse, Pia Abel Zur Wiesch, Sonke Andres, Robert A. Bonomo, Eva Choong, Fabrizio Clarelli, Laurent Decosterd, Andrew Dinardo, Hans-Peter Grobbel, Jan Heyckendorf, Doris Hillemann, Harald Hoffmann, Barbara Kalsdorf, Hande Karakose, Wiebke Knaack, Christina Koenig, Antal Martinecz, Florian Maurer, Matthias Merker, Stefan Niemann, Markus Nowak, Charles A. Peloquin, Patricia Sanchez Carballo, Dagmar Schaub, Thomas Schoen, Dominik Schwudke, Marga Teulen, Nick Verougstraete, Alain Verstraete, Franziska Waldow, Jim Werngren, Sebastian Georg Wicha, Christoph Lange
EUROPEAN RESPIRATORY JOURNAL
(2021)