Article
Chemistry, Multidisciplinary
Shogo Urakami, Hiroshi Hinou
Summary: The rapid analysis of glycoforms for glycoproteins is crucial in quality control and biomarker discovery. The direct analysis method using MALDI-ISDMS has shown high sensitivity within femtomolar range. The addition of 1,5-diaminonaphthalene (DAN) significantly improves the sensitivity and enables successful analysis of diluted biological samples.
Article
Materials Science, Ceramics
Aekgaran Sangmala, Patcharin Naemchanthara, Pichet Limsuwan, Kittisakchai Naemchanthara
Summary: The study found that using hydroxyapatite synthesized from chicken eggshell waste as a replacement for K-feldspar in ceramic fluxing agent can significantly improve the physical and mechanical properties of ceramic products, such as linear shrinkage and bulk density. Additionally, after heat treatment, the ceramic samples added with hydroxyapatite showed a significant decrease in apparent porosity and water absorption, approaching zero.
INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY
(2021)
Review
Engineering, Biomedical
Rosemond A. Mensah, Kawther Salim, Katarzyna Peszko, Seyta Diop, Ting H. R. Wong, David Y. S. Chau
Summary: Chicken eggshell membrane (ESM) is a rich resource with defined structural profile, chemical composition, and mechanical characteristics, which has potential applications in tissue regeneration, wound healing, and drug delivery. However, challenges remain in improving its mechanical properties, joining fragments, and enhancing its therapeutic capacity. This review provides an overview of ESM extraction, isolation, and characterization, as well as current and future applications in regenerative medicine.
BIOMEDICAL MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Tiantian Sun, Cong Xiao, Zhuliang Yang, Jixian Deng, Xiurong Yang
Summary: The avian eggshell is formed in the uterus, and changes in uterine function can affect eggshell quality. This study identified key genes and pathways involved in uterine functional maintenance in chickens through RNA sequencing and bioinformatics analysis. The differentially expressed genes were found to be enriched in the extracellular matrix and collagen-related pathways, and candidate genes for uterine functional maintenance were identified.
Article
Chemistry, Multidisciplinary
Pornsawai Praipipat, Pimploy Ngamsurach, Krissana Pratumkaew
Summary: The presence of lead in wastewater poses a threat to the quality of water, as well as the aquatic organisms and the environment. Therefore, proper wastewater treatment is necessary before discharging it into receiving water bodies. This study synthesized and investigated the efficiency of lead removal using chicken eggshell powder (CP) and chicken eggshell powder-doped iron (III) oxide-hydroxide (CPF) through various experiments. CPF demonstrated higher lead removal efficiency compared to CP, requiring less material dosage and contact time. Additionally, CPF showed promise as a reusable material for lead adsorption in industrial applications.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Engineering, Environmental
T. E. Odetoye, J. O. Agu, E. O. Ajala
Summary: Eggshell waste was investigated as a potential catalyst source for the transesterification of chicken fat, resulting in a maximum biodiesel yield of 90.2% that met ASTM standards. The study demonstrated that eggshell waste could be an affordable and effective catalyst for biodiesel production from chicken fat feedstock.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Agriculture, Dairy & Animal Science
Lin Xuan, Xuefeng Shi, Guiyun Xu, Jiangxia Zheng
Summary: This study used micro-CT technology to investigate the 3-dimensional microstructure of eggshells and found that the ratio of pore volume to mastoid space volume and the morphological characteristics of the pores have an impact on eggshell translucency. The porosity of the eggshell is also related to translucency.
Article
Biotechnology & Applied Microbiology
Jing Yang, Zhiqiong Mao, Xiqiong Wang, Jingjie Zhuang, Sijia Gong, Zhouyang Gao, Guiyun Xu, Ning Yang, Congjiao Sun
Summary: The study comprehensively investigated the regulatory mechanisms of eggshell brownness in different eggshell color and time following oviposition. Results suggest that Pp IX is synthesized de novo and stored in the shell gland, with ALAS1 identified as a key gene regulating Pp IX synthesis. Additionally, three transporters in the Pp IX pathway and three metabolites in shell glands and uterine fluid may influence eggshell browning.
Article
Biochemical Research Methods
Liang Chen, Joan M. Cabot, Brett Paull
Summary: A rapid, low-cost, and disposable microfluidic thread-based isotachophoresis method has been developed for the purification and preconcentration of nucleic acids, showing potential for the development of affordable analytical and diagnostic devices based on DNA and RNA isolation. This approach extracts protein-free DNA from electrolyte buffer and demonstrates efficient concentration with exclusion of PCR inhibitors, enabling detection of lambda DNA in whole blood within a linear range of 1.0-1 x 10(5) fg mu L-1.
Review
Chemistry, Multidisciplinary
Sanjay S. Timilsina, Pawan Jolly, Nolan Durr, Mohamed Yafia, Donald E. Ingber
Summary: The team developed an efficient antifouling coating for multiplexed EC sensors, addressing the sensitivity and selectivity loss caused by biological samples, and made significant progress.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Maeva Halgrain, Nelly Bernardet, Marine Crepeau, Nathalie Meme, Agnes Narcy, Maxwell Hincke, Sophie Rehault-Godbert
Summary: During chicken embryonic development, the eggshell plays a crucial role in skeleton calcification. The study explores the expression of candidate genes related to eggshell dissolution and transport of minerals to the embryo in the chorioallantoic membrane (CAM) and yolk sac (YS). The findings reveal differences in gene regulation between CAM and YS, highlighting the importance of understanding the molecular components involved in bone mineralization.
Review
Agriculture, Multidisciplinary
Leina Dou, Yingjie Zhang, Yuchen Bai, Yuan Li, Minggang Liu, Shibei Shao, Qing Li, Wenbo Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study discussed the application of chicken yolk antibody (IgY) in detecting chemical and biological hazards in food samples, presenting its advantages, structure, production methods, and principles of immunoassays. The future requires more types of IgY, increased sensitivity, and diversification in recognition-to-signal methods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Xue Cheng, Ying Ma, Xinghua Li, Yuchen Liu, Ruiqi Zhang, Yalan Zhang, Cuidie Fan, Lujiang Qu, Zhonghua Ning
Summary: Speckles are common on brown chicken eggshells, especially for old hens, and they are important for consumer preference and economic value. The cause of speckles in eggshells is unclear. In this study, the difference in eggshell quality between speckled eggs and normal eggs was verified. The results showed no significant difference in eggshell quality between the two, except for a lower eggshell color-L value in the speckled eggs. The uneven distribution of the eggshell pigment protoporphyrin IX is inferred to be the cause of speckled eggs.
Article
Energy & Fuels
Fekadu Ashine, Zebene Kiflie, Sundramurthy Venkatesa Prabhu, Belachew Zegale Tizazu, Venkatramanan Varadharajan, Manivasagan Rajasimman, Sang-Woo Joo, Yasser Vasseghian, Mani Jayakumar
Summary: The present study optimized the process of biodiesel production from Argemone mexicana oil using calcium oxide as a catalyst derived from chicken eggshells. The catalyst was characterized and the parameters were optimized through response surface methodology, resulting in a high yield of biodiesel. Non-statistical techniques were used to analyze the optimal values, and the obtained biodiesel was characterized using spectroscopic techniques.
Article
Biology
Feng Zhu, Zhong-Tao Yin, Qiang-Sen Zhao, Yun-Xiao Sun, Yu-Chen Jie, Jacqueline Smith, Yu-Ze Yang, David W. Burt, Maxwell Hincke, Zi-Ding Zhang, Meng-Di Yuan, Jim Kaufman, Cong-Jiao Sun, Jun-Ying Li, Li-Wa Shao, Ning Yang, Zhuo-Cheng Hou
Summary: This study assembled a chromosome-level reference genome of the Silkie chicken, which revealed the complete genomic sequences of important genes such as leptin, ovocleidin-17, and tumor-necrosis factor-alpha. It also provided annotation for the MHC region and gene expression information. Additionally, the study included whole-genome methylation and genetic variation maps, as well as the identification of a complex genetic region associated with fibromelanosis.
COMMUNICATIONS BIOLOGY
(2023)
Article
Engineering, Biomedical
Elena Filova, Marie Steinerova, Martina Travnickova, Jarmila Knitlova, Jana Musilkova, Adam Eckhardt, Daniel Hadraba, Roman Matejka, Simon Prazak, Jana Stepanovska, Johanka Kucerova, Tomas Riedel, Eduard Brynda, Alena Lodererova, Eva Honsova, Jan Pirk, Miroslav Konarik, Lucie Bacakova
Summary: The study demonstrated that modified decellularized porcine pericardium scaffolds, seeded with human adipose tissue-derived stem cells and facilitated with fibrin mesh, heparin, and VEGF, were able to be fully repopulated within days. The recellularization process by ASCs produced various extracellular matrix proteins in the scaffold. Additionally, thin layers of human umbilical endothelial cells formed rapidly on the scaffold surfaces pre-seeded with ASCs.
BIOMEDICAL MATERIALS
(2021)
Article
Cell Biology
J. Knitlova, M. Doubkova, Martin Plencner, David Vondrasek, Adam Eckhardt, Martin Ostadal, Jana Musilkova, Lucie Bacakova, Tomas Novotny
Summary: Minoxidil demonstrates an inhibitory effect on cell proliferation, collagen accumulation, collagen fiber assembly, and extracellular matrix contraction processes associated with clubfoot fibrosis in vitro. This suggests the potential therapeutic relevance of Minoxidil as an adjuvant pharmacological therapy for the treatment of relapsed clubfoot.
CONNECTIVE TISSUE RESEARCH
(2021)
Letter
Orthopedics
Tomas Novotny, Adam Eckhardt
JOURNAL OF PEDIATRIC ORTHOPAEDICS
(2021)
Article
Biochemistry & Molecular Biology
Hana Ujcikova, Lucie Hejnova, Adam Eckhardt, Lenka Roubalova, Jiri Novotny, Petr Svoboda
Summary: The study revealed that opioid addiction can lead to changes in protein composition in different regions of the brain, mainly related to energy metabolism and cytoskeleton reorganization. The down-regulation of 14-3-3 proteins in the rat cortex and hippocampus after 3 months of drug cessation was identified as the most important change.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Review
Chemistry, Analytical
Marine Morvan, Ivan Miksik
Summary: Determining the presence and position of D-amino acids in proteins is a complex challenge, requiring two strategies for detection. Enzymatic digestion and acid hydrolysis are the main methods for detecting D-amino acids. In order to improve the resolution of chiral separations, future work may focus on the discovery and development of new chiral stationary phases and derivatization reagents.
Article
Biochemistry & Molecular Biology
Jarmila Knitlova, Martina Doubkova, Adam Eckhardt, Martin Ostadal, Jana Musilkova, Lucie Bacakova, Tomas Novotny
Summary: The study focused on collagen deposition and crosslink changes in clubfoot tissue, successfully inhibiting these processes in vitro with the LOX inhibitor BAPN. The results suggest that BAPN has promising potential for the treatment of relapsed and resistant clubfoot.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Hermann Ehrlich, Magdalena Luczak, Rustam Ziganshin, Ivan Miksik, Marcin Wysokowski, Paul Simon, Irena Baranowska-Bosiacka, Patrycja Kupnicka, Alexander Ereskovsky, Roberta Galli, Sergey Dyshlovoy, Jonas Fischer, Konstantin R. Tabachnick, Iaroslav Petrenko, Teofil Jesionowski, Anna Lubkowska, Marek Figlerowicz, Viatcheslav N. Ivanenko, Adam P. Summers
Summary: Actin is an ancient intracellular protein that plays a crucial role in cell functions and also affects biosilica deposition in metazoans.
Article
Biochemistry & Molecular Biology
Hana Ujcikova, Adam Eckhardt, Lucie Hejnova, Jiri Novotny, Petr Svoboda
Summary: This study analyzed the changes in protein profiles in the rat hippocampus and forebrain cortex after 6-month morphine withdrawal. The results showed that the hippocampus was more susceptible to changes in protein and phosphoprotein profiles compared to the forebrain cortex, particularly in relation to metabolic changes, cytoskeleton organization, and response to oxidative stress.
Article
Multidisciplinary Sciences
Tomas Novotny, Adam Eckhardt, Martina Doubkova, Jarmila Knitlova, David Vondrasek, Eliska Vanaskova, Martin Ostadal, Jiri Uhlik, Lucie Bacakova, Jana Musilkova
Summary: The aim of this study was to investigate the expression of hypoxia-related proteins in the contracted side tissue of relapsed clubfoot, and we found an increase in the levels of hypoxia-related proteins and corresponding gene overexpression in the contracted side tissue. These results suggest that the hypoxia-associated pathway may contribute to the relapse of clubfoot by stimulating angioproliferation and fibroproliferation.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Marina Pozzolini, Ivan Miksik, Stefano Ghignone, Caterina Oliveri, Eleonora Tassara, Marco Giovine
Summary: This study describes the protein content of sponge spicules in Petosia ficiformis using a combination of transcriptomic and proteomic analysis. The results reveal a diverse group of proteins, including enzymes that may be related to lysosomes, suggesting a possible evolutionary origin of the biosilica machinery in sponges.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Analytical
Marine Morvan, Ivan Miks
Summary: Collagen, the most abundant protein in animal and human bodies, undergoes age-related changes such as increased hydrophobicity, post-translational modifications, and amino acid racemization. This study found that protein hydrolysis under deuterium conditions helps to limit natural racemization, preserving the homochirality of recent collagens. However, natural amino acid racemization was observed in aging collagens, indicating that the percentage of D-amino acids increases with age. The degradation of collagen over time leads to the loss of one-fifth of the sequence information. Post-translational modifications may explain the changes in hydrophobicity in aging collagens.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Veronika Gvozdikova Javurkova, Ivan Miksik
Summary: Recent research has found that maternal egg yolk components, which have effects on the developing embryo, are interconnected and co-adapted. However, there has been no study on the associations between maternal albumen steroids, proteins, and eggshell compounds. This study investigates these associations and reveals their potential impact on avian embryo and offspring phenotype.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2023)
Review
Chemistry, Analytical
Ivan Miksik, Marine Morvan, Jaroslav Bruzek
Summary: Proteomics has emerged as an attractive alternative to DNA analysis for studying human and animal material. It overcomes the limitations of DNA amplification in ancient samples, contamination, high cost, and limited preservation of nuclear DNA. Proteomics allows for relatively simple and cost-effective sex estimation by analyzing proteins preserved in tooth enamel, offering reduced destruction and minimum sample size requirements.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Physical
Samantha L. Flores-Lopez, Ana Arenillas, Ivan Miksik, J. Angel Menendez, Miguel A. Montes-Moran
Summary: This study evaluated the potential of novel reduced graphene oxide aerogels (rGO) as extractants of mixtures of three UV-benzotriazoles in water at low concentrations. The rGO aerogels containing 60% rGO performed the best, achieving recoveries of 80-100% when using tetrahydrofuran (THF) as an elution solvent. The method is simple, fast, and achieves a detection limit of 1 ng mL(-1).
Article
Polymer Science
Jana Svobodova, Ivan Miksik
Summary: Ethanol extracts of three widely growing plants were used as wall surface modifiers in inner quartz capillaries for the separation of nucleotides and amino compounds. The study aimed to investigate the possible interactions between physiological compounds and freely available plant extracts anchored in the sol-gel stationary phase. The results showed significant changes in the effective mobility of the analytes, highlighting the importance of the sol-gel additives.