Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 394, Issue 1, Pages 277-283Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2648-5
Keywords
Optical trapping; Microchip; Cell separation; Sheath flow; Trapping force
Funding
- Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government
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We investigated properties of cells affecting their optical trapping force and successfully established a novel cell separation method based on the combined use of optical trapping force and microfluidics on a microchip. Our investigations reveal that the morphology, size, light absorption, and refractive index of cells are important factors affecting their optical trapping force. A sheath flow of sample solutions created in a microchip made sample cells flow in a narrow linear stream and an optical trap created by a highly focused laser beam captured only target cells and altered their trajectory, resulting in high-efficiency cell separation. An optimum balance between optical trapping force and sample flow rate was essential to achieve high cell separation efficiency. Our investigations clearly indicate that the on-chip optical trapping method allows high-efficiency cell separation without cumbersome and time-consuming cell pretreatments. In addition, our on-chip optical trapping method requires small amounts of sample and may permit high-throughput cell separation and integration of other functions on microchips.
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