Article
Chemistry, Applied
Wanyi Dong, Chaoqing Tang, Minquan Xia, Long Sheng, Zhaoxia Cai
Summary: The egg yolk immunoglobulin loaded chitosan-liposomes prepared with the assistance of supercritical carbon dioxide showed better stability and high encapsulation efficiency, serving as an effective carrier to enhance human immunity.
Article
Plant Sciences
Yue-Rong Tan, Yuki Nakamura
Summary: Phosphatidylcholine is an essential phospholipid in plant growth. In Arabidopsis, a specific enzyme, phospholipid N-methyltransferase, plays an important role in developing seeds.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Food Science & Technology
Emmanuel Ayobami Oyeniyi, Carlos Arterio Sorgi, Luiz Gustavo Gardinassi, Lara Ferreira Azevedo, Joseph Adewuyi Adeyemi, Olumuyiwa Temitope Omotoso, Lucia Helena Faccioli, Lusania Maria Greggi Antunes, Fernando Barbosa Jr
Summary: The study investigated the toxicity of D. tripetala fruit extract on mice using lipidomic analyses, comet and Acetylcholinesterase (AChE) assays. The fruit extract did not induce DNA damage, but significantly affected body weight and brain AChE activity in the mice. Additionally, the extract modulated the profile of brain phospholipids, potentially through activation of inflammation and methylation pathways.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Danielle Defries, Kayla Curtis, Jay C. Petkau, Shiva Shariati-Ievari, Heather Blewett, Michel Aliani
Summary: After treatment with ALA, the incorporation of ALA into phospholipids is selective, with certain molecular species of phospholipids increasing almost immediately while others only increasing after several hours.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Nabangshu Sharma, Ries J. Langley, Chatchakorn Eurtivong, Euphemia Leung, Ryan Joseph Dixon, Emily K. Paulin, Shaun W. P. Rees, Lisa Pilkington, David Barker, Johannes Reynisson, Ivanhoe K. H. Leung
Summary: The study reports the development and optimisation of a MALDI-TOF mass spectrometry-based assay to monitor PC-PLCBc activity, comparing the inhibition of PC-PLCBc by different structural classes of inhibitors in one-phase and two-phase reaction systems. The assay was shown to have advantages over the commonly used Amplex Red assay and can be applied to study the activity and inhibition of other phospholipases.
ANALYTICAL METHODS
(2021)
Review
Agriculture, Dairy & Animal Science
Hanyi Shi, Xuejuan Deng, Xiaoyu Ji, Ning Liu, Huiyi Cai
Summary: Astaxanthin (Ax) and lutein are important fat-soluble pigments and essential nutrients for human and animal health. This review focuses on the commercial production of Ax and lutein, their effects on yolk color and nutritional functionality, and their enhancement of immune function. The potential roles of carotenoids in the cytokine storm and gut microbiota are also briefly presented, along with suggestions for future research.
Article
Agriculture, Dairy & Animal Science
Andrea Ianni, Dayana Bartolini, Francesca Bennato, Giuseppe Martino
Summary: This study characterizes the qualitative aspects of eggs obtained from the Nera Atriana hen and highlights significant differences compared to eggs from commercial hybrid hens, which may lead consumers to prefer these niche products.
Article
Agriculture, Dairy & Animal Science
Atefeh Berenjian, Seyed Davood Sharifi, Abdollah Mohammadi-Sangcheshmeh, Mohammad Reza Bakhtiarizadeh
Summary: This study investigated the effects of omega-3 fatty acids on egg production, nutrients digestibility, eggs yolk lipid peroxidation, and intestinal morphology in laying hens under physiological stress. The results showed that dietary omega-3 fatty acids had beneficial effects on gut morphology and nutrient digestibility, but could not alleviate the negative effects of physiological stress on performance.
Article
Biochemistry & Molecular Biology
Soyogu Yamashita, Naoki Kawada, Wei Wang, Kenta Susaki, Yumi Takeda, Mamoru Kimura, Yoshitaka Iwama, Yutaka Miura, Michihiro Sugano, Ryosuke Matsuoka
Summary: This study investigated the effect of consuming 300 mg of egg yolk choline per day on cognitive function in Japanese adults. The results showed that the intake of egg yolk choline improved verbal memory, which is a part of cognitive functions. However, more well-designed and large-scale studies are needed to confirm these effects.
LIPIDS IN HEALTH AND DISEASE
(2023)
Article
Food Science & Technology
Yaotong Liu, Yujie Chi, Yuan Chi
Summary: Adding CaCl2 to the salting solution can improve the quality of salted separated egg yolk by affecting the composition and structure of HDL and LDL. CaCl2 improves the orderliness of HDL structure, loosens the LDL structure, weakens the aggregation behavior of HDL, and prevents HDL and LDL from participating in the formation of a three-dimensional gel structure.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Microbiology
Fuxiao Wei, Xinyue Yang, Meihong Zhang, Chang Xu, Yongfei Hu, Dan Liu
Summary: This study investigated the effects of Akkermansia muciniphila (A. muciniphila) on lipid metabolism and egg quality in laying hens. The results showed that A. muciniphila supplementation could efficiently improve lipid metabolism, decrease body weight and fat deposition, and improve yolk lipid profile in laying hens. It also suggested that different preparations of A. muciniphila, including live, pasteurized, and lyophilized, have the potential for use as additives to improve laying hen production.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Ana Rey, Almudena De-Cara, Almudena Rebole, Ignacio Arija
Summary: Short-term supplementation of spirulina significantly affected the vitamin content and color of egg yolks. The strain of laying hens had a significant impact on the fatty acid composition of yolks, showing different responses to supplementation based on breed. Overall, spirulina supplementation had varied effects on egg quality parameters and nutritional value.
Article
Pharmacology & Pharmacy
Stefania-Claudia Jitaru, Andrei Neamtu, Gabi Drochioiu, Laura Darie-Ion, Iuliana Stoica, Brindusa-Alina Petre, Vasile-Robert Gradinaru
Summary: Peptides and their related compounds have the ability to form various nanostructures in response to stimuli, such as pH, temperature, and ionic strength. In this study, a pentapeptide derived from lysozyme was synthesized and characterized for its fibrillar structures. The peptide was synthesized using solid phase peptide synthesis and its properties were confirmed using spectroscopy and microscopy. The obtained fibrils show potential for medical and cosmetic applications.
Article
Agriculture, Dairy & Animal Science
Dieudonne M. Dansou, Hao Wang, Ramdhan D. Nugroho, Weizhao He, Qingyu Zhao, Junmin Zhang
Summary: The study evaluated the effects of moderate and high dose dietary supplementation of astaxanthin on egg quality and laying hens' health status. It was found that moderate dose supplementation efficiently improved egg yolk color and laying hens' health status, while high dose supplementation showed positive effects but with decreased efficacy compared to moderate doses. Astaxanthin had no adverse effects on laying hens' performance and health status but may not be valuable for egg fortification at high doses.
Article
Chemistry, Multidisciplinary
Valeria Conti Nibali, Caterina Branca, Ulderico Wanderlingh, Giovanna D'Angelo
Summary: This study investigated the vibrational signature of two different phospholipids in the far-infrared region to evaluate the effect of headgroup composition on intermolecular interactions. The presence of additional hydrogen bonds in phosphatidylethanolamine (PE) compared to phosphatidylcholine (PC) affected water penetration and severely reduced the hydration capability of PE lipids.
APPLIED SCIENCES-BASEL
(2021)
Letter
Hematology
Julia Froebel, Sonja Hartwig, Sabine Jourdain, Johannes C. Fischer, Christoph Zilkens, Andrea Kuendgen, Detlev Suckau, Ulrich Germing, Akos Czibere, Stefan Lehr
AMERICAN JOURNAL OF HEMATOLOGY
(2015)
Article
Biochemical Research Methods
Rita Casadonte, Mark Kriegsmann, Soeren-Oliver Deininger, Kerstin Amann, Rainer Paape, Eckhard Belau, Detlev Suckau, Jens Fuchser, Janine Beckmann, Michael Becker, Joerg Kriegsmann
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Robert Popp, David Malmstroem, Andrew G. Chambers, David Tin, Alexander G. Camenzind, J. Grace van der Gugten, Daniel T. Holmes, Michael Pugia, Marta Jaremek, Shannon Cornett, Detlev Suckau, Christoph H. Borchers
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2015)
Review
Biochemical Research Methods
Jan Wiesner, Anja Resemann, Catherine Evans, Detlev Suckau, Wolfgang Jabs
EXPERT REVIEW OF PROTEOMICS
(2015)
Article
Biochemical Research Methods
Irene van den Broek, Jan Nouta, Morteza Razavi, Richard Yip, Marco R. Bladergroen, Fred P. H. T. M. Romijn, Nico P. M. Smit, Oliver Drews, Rainer Paape, Detlev Suckau, Andre M. Deelder, Yuri E. M. van der Burgt, Terry W. Pearson, N. Leigh Anderson, Christa M. Cobbaert
Article
Cell Biology
Alice Ly, Cedrik Schoene, Michael Becker, Janine Rattke, Stephan Meding, Michaela Aichler, Detlev Suckau, Axel Walch, Stefanie M. Hauck, Marius Ueffing
HISTOCHEMISTRY AND CELL BIOLOGY
(2015)
Article
Biochemical Research Methods
N. Leigh Anderson, Morteza Razavi, Terry W. Pearson, Gary Kruppa, Rainer Paape, Detlef Suckau
JOURNAL OF PROTEOME RESEARCH
(2012)
Article
Medicine, Research & Experimental
Daniel Ayoub, Wolfgang Jabs, Anja Resemann, Waltraud Evers, Catherine Evans, Laura Main, Carsten Baessmann, Elsa Wagner-Rousset, Detlev Suckau, Alain Beck
Article
Biochemical Research Methods
Melanie Lagarrigue, Michael Becker, Regis Lavigne, Soeren-Oliver Deininger, Axel Walch, Florence Aubry, Detlev Suckau, Charles Pineau
MOLECULAR & CELLULAR PROTEOMICS
(2011)
Letter
Biotechnology & Applied Microbiology
Matthew C. Chambers, Brendan Maclean, Robert Burke, Dario Amodei, Daniel L. Ruderman, Steffen Neumann, Laurent Gatto, Bernd Fischer, Brian Pratt, Jarrett Egertson, Katherine Hoff, Darren Kessner, Natalie Tasman, Nicholas Shulman, Barbara Frewen, Tahmina A. Baker, Mi-Youn Brusniak, Christopher Paulse, David Creasy, Lisa Flashner, Kian Kani, Chris Moulding, Sean L. Seymour, Lydia M. Nuwaysir, Brent Lefebvre, Frank Kuhlmann, Joe Roark, Paape Rainer, Suckau Detlev, Tina Hemenway, Andreas Huhmer, James Langridge, Brian Connolly, Trey Chadick, Krisztina Holly, Josh Eckels, Eric W. Deutsch, Robert L. Moritz, Jonathan E. Katz, David B. Agus, Michael MacCoss, David L. Tabb, Parag Mallick
NATURE BIOTECHNOLOGY
(2012)
Article
Biochemical Research Methods
Rita Casadonte, Mark Kriegsmann, Friederike Zweynert, Katrin Friedrich, Gustavo Bretton, Mike Otto, Soeren-Oliver Deininger, Rainer Paape, Eckhard Belau, Detlev Suckau, Daniela Aust, Christian Pilarsky, Joerg Kriegsmann
Article
Medicine, Research & Experimental
Anja Resemann, Lily Liu-Shin, Guillaume Tremintin, Arun Malhotra, Adam Fung, Fang Wang, Gayathri Ratnaswamy, Detlev Suckau
Article
Chemistry, Analytical
Christoph Gstottner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer, Elena Dominguez-Vega
Summary: The study provides insights into the structural and functional characteristics of recombinant RBDs expressed in CHO and HEK293 cells, revealing differences in glycosylation patterns. By employing a multilevel mass spectrometric approach, the researchers comprehensively annotated the proteoforms of RBDs, allowing for a detailed analysis of their post-translational modifications. The work not only addressed the binding properties of RBDs to SARS-CoV-2 antibodies and ACE2 receptor, but also established an analytical workflow for characterization and batch-to-batch comparison of new RBDs.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Ulrike Anders, Maya Gulotti-Georgieva, Susann Zelger-Paulus, Fatima-Ezzahra Hibti, Chiraz Frydman, Detlev Suckau, Roland K. O. Sigel, Renato Zenobi
Summary: The study combined SPR imaging with MALDI MS to improve the identification of potential RNA binders, allowing for high-throughput analysis of complex mixtures. Results showed that in addition to Mss116, other proteins in crude yeast mitochondrial lysate may interact with the intronic RNA, suggesting that the folding and splicing of the intron may be regulated by multiple cofactors in vivo.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Jakob Meier-Credo, Laura Preiss, Imke Wiillenweber, Anja Resemann, Christoph Nordmann, Jure Zabret, Detlev Suckau, Hartmut Michel, Marc M. Nowaczyk, Thomas Meier, Julian D. Langer
Summary: Identification and sequence determination of soluble proteins by mass spectrometry have become routine analyses. However, the analysis of membrane proteins remains challenging due to their hydrophobicity and poor annotation. This study introduces a guide for the mass spectrometric characterization of small membrane proteins and demonstrates a new method for purifying and sequencing these proteins.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)