Article
Chemistry, Multidisciplinary
Anna Roszkowska, Natalia Treder, Alina Plenis, Natalia Miekus, Ilona Oledzka, Piotr Kowalski, Tomasz Baczek
Summary: This study compared and optimized two microextraction strategies for the extraction of four immunosuppressants in biofluids. The results showed that one strategy exhibited better performance for the extraction of a novel immunosuppressive drug.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Agriculture, Multidisciplinary
Myriam Bustamante-Rangel, Maria Milagros Delgado-Zamarreno, Encarnacion Rodriguez-Gonzalo
Summary: A simple and sensitive method has been developed for the analysis of anthelmintic drugs in milk, involving extraction using QuEChERS method and separation and determination by LC-MS/MS. This method provides high sensitivity for the determination of anthelmintics in milk samples and can be successfully used for routine analysis.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Environmental Sciences
Marina Bellot, Carlos Barata, Cristian Gomez-Canela
Summary: The study evaluated the stability of 17 neuroactive compounds in water and found that most compounds did not degrade, except for apomorphine and 6-hydroxydopamine which degraded completely within 24 hours. Most compounds altered motile responses to light in the crustacean Daphnia magna.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Pharmacology & Pharmacy
Alexandr Gish, Veronique Dumestre-Toulet, Camille Richeval, Jean-Francois Wiart, Florian Hakim, Delphine Allorge, Jean-Michel Gaulier
Summary: This study reports the pharmaceutical design of new psychoactive substances (NPS) detected in oral fluid from drivers during a music festival in 2019. The results show an increase in the proportion of synthetic cannabinoids and cathinone derivatives, and a decrease in cyclohexanones.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Biochemical Research Methods
Nadia Arbouche, Jean-Sebastin Raul, Pascal Kintz
Summary: Oral antidiabetics are drugs used to control blood sugar in diabetic subjects. The greatest risk of using these drugs is hypoglycemia, especially in individuals with no history of exposure to these medications. Hair testing for oral antidiabetics can help differentiate the cause of hypoglycemia and has a large detection window with long-term stability. A liquid-chromatography coupled to tandem mass spectrometry method has been developed and successfully applied to analyze 13 oral antidiabetics in hair samples from diabetic patients.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Dong Xiang, Tinghui Zhao, Jue Wang, Yang Cao, Qiuxia Yu, Lu Liu, Hengyi Yu, Xiping Li, Ninghong Li, Yang Yi, Xuepeng Gong
Summary: A sensitive and robust LC-MS/MS method was developed and validated for the quantification of olverembatinib in human plasma and cerebrospinal fluid (CSF). The method involved liquid-liquid extraction and precipitation enrichment for plasma and CSF pretreatment, respectively. The detection was performed by electrospray ionization in a positive ion mode using multiple reaction monitoring. The method proved to be accurate and precise, with a successful application in measuring olverembatinib concentrations in plasma and CSF from chronic myeloid leukemia patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Sunyeong Jang, Hyungju Seo, Hojin Kim, Hyoyoung Kim, Jongsung Ahn, Hyunjeong Cho, Sunghie Hong, Seunghwa Lee, Taewoong Na
Summary: A method for the rapid and quantitative analysis of 30 veterinary drugs in feed samples was developed and validated. The method showed good linearity and recovery rates, and can be used for screening residual multiclass veterinary drugs in feed samples.
Article
Biochemical Research Methods
Kaue de Oliveira Chinaglia, Ana Carolina Furiozo Arantes, Kelly Francisco da Cunha, Eduardo Geraldo de Campos, Julia Martinelli Magalhaes Kahl, Leonardo Costalonga Rodrigues, Jose Luiz Costa
Summary: This study developed a quantitative method for analyzing methylphenidate and its metabolite ritalinic acid in oral fluid. The method showed satisfactory validation results and was successfully applied to real samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Piotr Adamowicz, Joanna Bigosinska, Dominika Gil, Marta Suchan, Bogdan Tokarczyk
Summary: This study demonstrates the usefulness of fingerprints as an alternative material in forensic toxicology. Drugs can be detected in fingerprints even after single doses, with parent compounds dominating over metabolites in fingerprints. The detection window depends on the type of drug and duration of the treatment.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Review
Biochemistry & Molecular Biology
Cristian D'Ovidio, Marcello Locatelli, Miryam Perrucci, Luigi Ciriolo, Kenneth G. Furton, Isil Gazioglu, Abuzar Kabir, Giuseppe Maria Merone, Ugo de Grazia, Imran Ali, Antonio Maria Catena, Michele Treglia, Luigi T. Marsella, Fabio Savini
Summary: LC-MS/MS plays a crucial role in screening and tracking pharmacotoxicological and forensic cases due to its advanced features. This review paper focuses on its applications in pharmacotoxicology and highlights its importance in the development of pharmacological and forensic research. It discusses therapeutic drug monitoring and clinical approaches as well as the determination of illicit drugs.
Article
Environmental Sciences
Weimin Wang, Changsheng Guo, Like Chen, Ziwen Qiu, Xingxing Yin, Jian Xu
Summary: An enantioselective method was developed to quantify amphetamine-type chiral illicit drugs in wastewater and surface water samples from a wastewater treatment plant and its effluent-receiving river in Beijing, China. The method showed excellent performance in monitoring the variations of enantiomers in the wastewater treatment process.
ENVIRONMENTAL POLLUTION
(2021)
Article
Medical Laboratory Technology
Gamze Avcioglu, Gulsen Yilmaz, Safak Yalcin Sahiner, L. Didem Kozaci, Ceylan Bal, Fatma Meric Yilmaz
Summary: This study evaluated the analytical performance of the Kite Biotechnology Oral fluid screening test device, finding good sensitivity for cocaine, amphetamines, and opiates, but insufficient sensitivity for methamphetamine/MDMA, benzodiazepines, and cannabis.
CLINICAL BIOCHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Anne Pourquet, Jordan Teoli, Aurore Bouty, Lucie Renault, Florence Roucher, Delphine Mallet, Chantal Rigaud, Frederique Dijoud, Pierre Mouriquand, Pierre-Yves Mure, Damien Sanlaville, Rene Ecochard, Ingrid Plotton
Summary: Determining steroid levels in the amniotic fluid provides insights into fetal adrenal and gonadal functions. This study aimed to establish reference ranges for 12 steroids during pregnancy and compare them to levels in pregnancies with 21-hydroxylase deficiency. The researchers used liquid chromatography coupled with tandem mass spectrometry to analyze amniotic fluid samples from control pregnancies and pregnancies with 21-hydroxylase deficiency. They found significant differences in certain steroids levels based on fetal sex and established age- and sex-dependent reference values. The study provides valuable information for prenatal diagnosis and the understanding of variations in fetal steroidogenesis.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Chemistry, Medicinal
Viviana Carmen Ciuca, Carmen Otilia Rusanescu, Victor Viorel Safta
Summary: The aim of this study was to analyze the depletion of tiamulin residues and metabolites in animal tissues after tiamulin administration and determine withdrawal times for products of animal origin. The results showed higher levels of tiamulin residues in liver compared to muscle in pigs, rabbits, and birds. The minimum withdrawal times for pigs, rabbits, and turkeys were found to be 5 days, 3 days for broiler chickens, and 0 days for eggs.
Article
Chemistry, Medicinal
M. Carmen Louzao, Paula Abal, Celia Costas, Toshiyuki Suzuki, Ryuichi Watanabe, Natalia Vilarino, Ana M. Botana, Mercedes R. Vieytes, Luis M. Botana
Summary: Okadaic acid and its structural analogs DTX1 and DTX2 are marine lipophilic phycotoxins that can cause diarrheic shellfish poisoning. Studies found a dose-dependency in gastrointestinal absorption of these toxins and a different toxicokinetic pathway between them. The highest toxin concentration was detected in the stomach 24 hours post-administration, providing information for human safety assessment.
Article
Chemistry, Analytical
Bernardino Barcelo, Isabel Gomila, Ana de-Castro-Rios, Jon Perez-Barcena, Carmen Jimenez, Elena Lendoiro, Miguel Angel Elorza
Summary: Scopolamine is a versatile drug used for various purposes, but overdose can lead to diminished consciousness. The concentration of scopolamine in serum can explain sudden clinical presentations, while its presence in urine can be detected up to 48 hours. Therefore, screening for scopolamine and other toxins is crucial in identifying potentially life-threatening causes.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2021)
Article
Chemistry, Analytical
Natalia A. Platosz, Tina M. Binz, Markus R. Baumgartner, Elena Lendoiro, Ana de Castro, Marta Concheiro
Summary: The use and misuse of synthetic and prescription opioids in the USA has become an epidemic, leading to a high number of deaths and cases of opioid use disorder. Hair is now being increasingly used in forensic toxicology to investigate drug use patterns due to its long window of detection. A method was developed to detect and quantify 27 classic, prescription, and synthetic opioids in hair using LC-MS-MS.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Marta Concheiro, Frank Martin Gutierrez, Alejandro Ocampo, Elena Lendoiro, Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Patricia Penas-Silva, Manuel Macias-Cortina, Angelines Cruz-Landeira, Manuel Lopez-Rivadulla, Ana de-Castro-Rios
Summary: Analyzing prenatal marijuana exposure in paired hair, meconium, umbilical cord, and placental samples revealed complementarity between meconium and hair, with higher agreement with meconium for umbilical cord and placental results. THCCOOH-glucuronide in umbilical cord and placenta can be a biomarker of cannabis exposure, offering a potential alternative to meconium.
DRUG TESTING AND ANALYSIS
(2021)
Article
Toxicology
Angela Lopez-Rabunal, Elena Lendoiro, Marta Concheiro-Guisan, Eva Gonzalez-Colmenero, Patricia Penas-Silva, Ana Concheiro-Guisan, Manuel Macia-Cortinas, Manuel Lopez-Rivadulla, Ana De-Castro-Rios, Angelines Cruz
Summary: This study compared meconium, maternal hair, and maternal medical records to detect exposure to antidepressants and benzodiazepines during pregnancy. Maternal hair was more sensitive in detecting antidepressant use, while meconium analysis was better at providing detection of neonatal benzodiazepines exposure at delivery. No significant influence of prenatal exposure to these drugs on neonatal outcomes was observed.
FORENSIC TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Angela Lopez-Rabunal, Daniele Di Corcia, Eleonora Amante, Marta Massano, Angelines Cruz-Landeira, Ana De-Castro-Rios, Alberto Salomone
Summary: New psychoactive substances are emerging in the market, leading to potential risks for women, including pregnant women. It is crucial to develop analytical methods for detecting prenatal exposure to these substances. In a study involving 30 meconium samples, four tested positive for fentanyl and two tested positive for acetylfentanyl.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Correction
Biochemical Research Methods
Angela Lopez-Rabunal, Daniele Di Corcia, Eleonora Amante, Marta Massano, Angelines Cruz-Landeira, Ana de-Castro-Rios, Alberto Salomone
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lucia Paniagua-Gonzalez, Elena Lendoiro, Esteban Otero-Anton, Manuel Lopez-Rivadulla, Ana de-Castro-Rios, Angelines Cruz
Summary: This study aimed to perform a clinical validation for tacrolimus and mycophenolic acid in both DBS and MitraTM VAMS, fulfilling both analytical and clinical acceptance criteria. VAMS showed lower concentrations compared to routine matrices but met the analytical requirements after correction.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Medicine, Legal
Pablo Prego-Meleiro, Gemma Montalvo, Carmen Garcia-Ruiz, Begona Bravo Serrano, Sara Ayuso-Tejedor, Carmen Megia Morales, Oscar Quintela-Jorge
Summary: This study sheds light on previously unexplored contexts, experiences, and victim profiles in drug-facilitated sexual assault cases, including domestic cohabitation, labor, education, healthcare, women trafficking, and the daily life of people with intellectual disabilities. The new working framework applied in analyzing data from the National Institute of Toxicology and Forensic Sciences in Madrid from 2012 to 2017 expands the scope of research beyond the limited focus on young women in nightlife and party culture.
JOURNAL OF FORENSIC AND LEGAL MEDICINE
(2022)
Article
Toxicology
Matilde Zaballos, Ignacio Fernandez, Lucia Rodriguez, Sergio Garcia, Olalla Varela, Oscar Quintela, Maria-Jose Anadon, Jesus Almendral
Summary: Ropivacaine has been considered safe, but this study found that it alters ventricular conduction parameters and increases the risk of ventricular arrhythmias. Intravenous lipid emulsions do not prevent pacing-induced ventricular arrhythmias, but they improve resuscitation success rate and termination of arrhythmias.
CLINICAL TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Laura DeFreitas, Ana Miguel Fonseca Pego, Robert Kronstrand, Elena Lendoiro, Ana De Castro-Rios, Marta Concheiro
Summary: Identification and analysis of designer benzodiazepines have become a challenge in forensic toxicology. A fast and sensitive method using LC-MS/MS was developed and validated to determine 17 designer benzodiazepines in hair samples.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Jose Angel Lema-Atan, Elena Lendoiro, Lucia Paniagua-Gonzalez, Angelines Cruz, Manuel Lopez-Rivadulla, Ana de-Castro-Rios
Summary: The increased usage of cytostatic drugs poses a high risk of exposure among healthcare workers. This study describes the development and full validation of two liquid chromatography-tandem mass spectrometry methods for the detection of multiple drugs in working surfaces and urine samples. These validated methods allow for a comprehensive evaluation of both environmental and biological contamination in hospital settings and healthcare workers.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2023)