Article
Chemistry, Analytical
Yan Lu, Junmin Li, Yuqiao Liu, Ling Zhu, Shenghao Xiao, Mingxia Bai, Dajing Chen, Tian Xie
Summary: A biosensor based on ZIF-67 loaded with bi-enzymes of glucose (GOx) and hexokinase (HEX) was developed for effective detection of low concentrations of adenosine-5'-triphosphate (ATP), enabling real-time monitoring of ATP released by cells.
Article
Biophysics
Matias Regiart, Ana Ledo, Eliana Fernandes, German A. Messina, Christopher M. A. Brett, Mauro M. Bertotti, Rui Barbosa
Summary: Highly sensitive and selective nanostructured lactate and glucose microbiosensors were developed for simultaneous determination in rat brain. The microbiosensors, based on carbon fiber microelectrodes modified with nanoporous gold, exhibited enhanced sensitivity and electrocatalytic properties towards H2O2 detection. The selective measurements were achieved by enzyme immobilization, permselective layer, and a null sensor. The microbiosensor platform was used to determine lactate and glucose in blood serum samples, as well as in vivo measurements of basal extracellular concentrations in different rat brain structures.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Serban F. Peteu, Skye A. Russell, James J. Galligan, Greg M. Swain
Summary: The ATP biosensor described in this paper utilizes glucose oxidase and hexokinase immobilized within the electroactive polymer PEDOT, achieving indirect detection of ATP at different detection potentials with good detection performance.
Article
Dentistry, Oral Surgery & Medicine
Kotaro Sato, Ming Yang, Kae Nakamura, Hiromasa Tanaka, Masaru Hori, Miki Nishio, Akira Suzuki, Hideharu Hibi, Shinya Toyokuni
Summary: This study found that plasma-activated Ringer's lactate solution (PAL) can specifically kill oral squamous cell carcinoma (OSCC) cells and prevent carcinogenesis by changing collagenous matrix formation via LOX suppression, thus improving the survival rate of oral cancer.
Article
Chemistry, Multidisciplinary
Serge Ruccolo, Gilmar Brito, Melodie Christensen, Tetsuji Itoh, Keith Mattern, Kevin Stone, Neil A. Strotman, Alexandra C. Sun
Summary: This study reports a simplified cellular respiration mimic method that uses electricity to recycle enzymes for ATP generation. The method is simple, robust, and scalable, and applicable to complex enzymatic processes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biotechnology & Applied Microbiology
Anne Usvalampi, He Li, Alexander D. Frey
Summary: This study established a fully enzymatic route for synthesizing glucose 6-phosphate from cellulose, achieving a high conversion rate under optimized conditions. However, in the reaction involving cellulase release of glucose from cellulose, a high conversion rate was attained but at the cost of lower yield of glucose 6-phosphate.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Senzekile Majola, Myalowenkosi Sabela, Robert Moonsamy Gengan, Talent Raymond Makhanya
Summary: An electrochemical biosensor was developed for the indirect detection of Adenosine triphosphate (ATP) by immobilizing multiwalled carbon nanotubes decorated with pyrazole-capped selenium nanoparticles and dual enzyme reaction onto the surface of a glassy carbon electrode. The biosensor showed excellent sensitivity, stability, and reproducibility, with a detection limit of 0.015 mM and a quantification limit of 0.050 mM for ATP in spiked tablets. The success of green synthesis of the selenium nanoparticles was confirmed through various analyses.
CURRENT ANALYTICAL CHEMISTRY
(2023)
Review
Cell Biology
Sarah Weksler-Zangen
Summary: This article discusses the proper diagnosis and treatment of diabetes in patients with inherited mitochondrial diseases, as well as screening for the condition in family members. The severity of mitochondrial-related diabetes depends on the location of the mutation and the number of affected mitochondria copies. The preferred treatment for diabetes in patients with mitochondrial disorders is SGLT-2i and mitochondrial GLP-1-related substances.
Review
Endocrinology & Metabolism
Suraj Kubihal, Alpesh Goyal, Yashdeep Gupta, Rajesh Khadgawat
Summary: This article provides an overview of methods for measuring glucose in body fluids, including the types of glucose that can be measured in various body fluids, the importance of analyzing and evaluating laboratory reports. It also introduces the advantages of using continuous glucose monitoring systems for monitoring glucose in interstitial fluid and the rare use of glucose measurement in urine samples.
DIABETES & METABOLIC SYNDROME-CLINICAL RESEARCH & REVIEWS
(2021)
Article
Biochemical Research Methods
Giada Caniglia, Andrea Teuber, Holger Barth, Boris Mizaikoff, Christine Kranz
Summary: This study investigates the relationship between the adhesion properties of polydopamine films and their surface charge density and pH using atomic force microscopy and attenuated total reflection-Fourier transform infrared spectroscopy. The study also explores the impact of polydopamine surface charge density on bacterial adhesion and biofilm formation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Nikhil Arya, Tom Philipp, Simon Greiner, Michael Steiner, Christine Kranz, Montaha Anjass
Summary: The reversible electrodeposition of molecular vanadium oxide clusters and the formation of thin films are reported. The reversibility is found to be dependent on the reduction potential. The multi-electron reduction of the polyoxovanadate cluster facilitates the reversible formation of potassium vanadium oxide thin films.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Optics
Dominik Theiner, Benedikt Limbacher, Michael Jaidl, Marie Ertl, Michael Hlavatsch, Karl Unterrainer, Boris Mizaikoff, Juraj Darmo
Summary: We present a flexible molecular sensing platform in the terahertz range, achieved by merging near-infrared electro-optic modulation and photomixing technologies. The platform utilizes a new generation of compact gas cells called substrate-integrated hollow waveguides (iHWGs), which have been developed for the mid-infrared. We demonstrate the suitability of iHWGs for the terahertz domain by showcasing their low propagation losses and measuring rotational transitions of nitrous oxide (N2O). A fast frequency sideband modulation technique is employed, resulting in significantly reduced measurement times and increased accuracy compared to a standard wavelength tuning method.
Article
Chemistry, Multidisciplinary
Andrea Teuber, Giada Caniglia, Michael Wild, Matthias Godejohann, Christine Kranz, Boris Mizaikoff
Summary: Diamond thin-film waveguides with a nanocrystalline diamond layer were used to detect the IR signature of caffeine in the mid-infrared regime. The morphological properties of the waveguides were characterized using AFM and SEM, and theoretical simulations confirmed the feasibility of using a larger sensing area compared to strip waveguides. A comparative analysis confirmed the performance of the diamond thin-film-waveguide-based sensing system, indicating its potential for various applications.
Review
Chemistry, Physical
Andreas Hellmann, Annika Schundner, Manfred Frick, Christine Kranz
Summary: ATP is a widely distributed extracellular signaling molecule that regulates various physiological and pathological processes through purinergic receptor activation. Accurate measurement of ATP release is crucial for understanding transmission mechanisms, requiring direct, sensitive, non-invasive detection with high spatial and temporal resolution. This opinion article summarizes recent research on ATP detection using electrochemical methods and provides examples of how in vitro electrochemical ATP measurements contribute to insights in purinergic signaling, specifically in alveolar homeostasis and ATP's role as a major excitatory neurotransmitter in the central nervous system.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Andrea Teuber, Giada Caniglia, Holger Barth, Christine Kranz, Boris Mizaikoff
Summary: Bacterial sensing based on quantum cascade laser spectroscopy coupled with diamond or gallium arsenide thin-film waveguides is a novel approach for obtaining high-resolution infrared spectroscopic information of bacteria. In this study, Escherichia coli was used as an example, and the laser spectroscopy was compared to conventional Fourier transform infrared spectroscopy. The proliferation behavior of E. coli on the surfaces was also investigated using atomic force microscopy and scanning electron microscopy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Fatemehsadat Rahide, Krishnaveni Palanisamy, Jackson K. Flowers, Junjie Hao, Helge S. Stein, Christine Kranz, Helmut Ehrenberg, Sonia Dsoke
Summary: The naturally occurring amorphous Al2O3 film on an Al substrate poses a challenge for the performance of rechargeable Al batteries. This insulating Al oxide slows down electrode activation and hinders Al plating/stripping. The two sides of Al foils have different surface properties, with the non-shiny side having higher roughness and greater concentration of active sites. Various immersion pretreatments can modify the surface properties and create an interphase layer rich in Al, Cl, and N.
Article
Electrochemistry
Noha Sabi, Krishnaveni Palanisamy, Fatemehsadat Rahide, Sven Daboss, Christine Kranz, Sonia Dsoke
Summary: Investigated the impact of aluminum foil surface properties on the electrochemical behavior in aluminum battery half-cells. The purity, hardness and thickness of commercial aluminum foils influence the battery performance, with the 0.075 mm thickness showing the best cycling stability.
BATTERIES & SUPERCAPS
(2023)
Article
Electrochemistry
Krishnaveni Palanisamy, Sven Daboss, David Schaefer, Marcus Rohnke, Laurin Derr, Marcel Lang, Rolf Schuster, Christine Kranz
Summary: This study presents a cost-effective spray-coating process for the preparation of HC composite electrodes for sodium-ion batteries. The spray-coated anodes showed improved performance compared to doctor bladed anodes, indicating the potential of this method for practical applications.
BATTERIES & SUPERCAPS
(2023)
Article
Chemistry, Analytical
Michael Hlavatsch, Andrea Teuber, Max Eisele, Boris Mizaikoff
Summary: In this study, the combination of a tunable broadband mid-infrared femtosecond laser source with different sensors for liquid and gas samples is demonstrated. The light source is highly sensitive and suitable for quantitative analysis.
ACS MEASUREMENT SCIENCE AU
(2023)
Article
Chemistry, Analytical
Tingting Zhang, Rui Lu, Gongying Wang, Xiuyun Sun, Jiansheng Li, Boris Mizaikoff
Summary: A facile and sensitive glucose sandwich assay using surface-enhanced Raman scattering (SERS) was developed. Glucose was captured by 3-aminopheyonyl boronic acid (APBA) modified Ag nanoparticles decorated onto a polyamide surface. APBA forms specific cis-diol compounds with glucose molecules avoiding interference by other saccharides and biomolecules in urine enabling its selective detection. AEPO exhibited a distinctive SERS peak in the Raman silent region, thus increasing the sensitivity of the glucose detection to 10(-11) M. The developed SERS assay was reusable and showed potential as a diagnostic tool for glucose sensing in clinical applications.
Article
Chemistry, Analytical
Andrea Teuber, Giada Caniglia, Christine Kranz, Boris Mizaikoff
Summary: Diamond thin-film waveguides combined with quantum cascade lasers augmented by drop-casted graphene enable surface-enhanced infrared absorption spectroscopy, enhancing the signal for analytical scenarios with small sample volumes and low analyte concentration levels.
