Article
Biophysics
Eva Gonzalez-Fernandez, Matteo Staderini, Jamie R. K. Marland, Mark E. Gray, Ahmet Ucar, Camelia Dunare, Ewen O. Blair, Paul Sullivan, Andreas Tsiamis, Stephen N. Greenhalgh, Rachael Gregson, Richard Eddie Clutton, Stewart Smith, Jonathan G. Terry, David J. Argyle, Anthony J. Walton, Andrew R. Mount, Mark Bradley, Alan F. Murray
Summary: The study introduced a miniaturised, self-contained electrochemical pH sensor system using a methylene blue redox reporter, which demonstrated remarkable robustness, accuracy, and sensitivity. The sensor effectively tracked real-time intratumoral tissue pH changes in vivo, correlating well with laboratory blood tests.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Natalia Soares Prado, Luiz Andre Juvencio Silva, Regina Massako Takeuchi, Eduardo Mathias Richter, Andre Luiz dos Santos, Eduardo Henrique Lago Falcao
Summary: In this study, inexpensive graphite sheets were used as conductive substrates and modified to enhance the sensitivity for nitrofurantoin. The proposed sensor successfully quantified nitrofurantoin in tap water and urine samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Xiaoqian Wei, Shanlin Wang, Yujuan Zhan, Tianhan Kai, Ping Ding
Summary: We report a methylene blue-modified electrochemical aptamer sensor for determining microcystin-LR. The sensor signal transduction is based on changes in conformation and position of methylene blue induced by binding with microcystin-LR. We optimized the parameters and evaluated the sensor's electrochemical performance using square wave voltammetry. The reliability of the method was verified by testing environmental samples, and the sensor was shown to be reusable and regenerable with good accuracy and reproducibility. This rapid, reagent-free, and sensitive sensing platform will facilitate routine monitoring of microcystin-LR.
Article
Chemistry, Analytical
Dominica Kappo, Dmitry Shurpik, Pavel Padnya, Ivan Stoikov, Alexey Rogov, Gennady Evtugyn
Summary: In this study, a novel electrochemical DNA sensor was developed for the determination of antitumor drugs. The sensor showed excellent sensitivity and specificity for the detection of doxorubicin, with a detection limit of 0.13 pM. The sensor was also successfully tested on spiked samples of human plasma and doxorubicin medication.
Article
Chemistry, Analytical
Chengli Zhang, Xun You, Yuliang Li, Yuanyuan Zuo, Wei Wang, DanDan Li, ShengWei Huang, Hangzhi Hu, Feng Yuan, Fuqiang Shao, Minggang Yin
Summary: In this study, an aptamer-based amplification strategy using Ce-MOF and m-PdNFs-G4-MBs nanocomposite was developed for the sensitive detection of dopamine (DA). The results showed that the new detection platform achieved a minimum detection limit of 6 pmol/L for DA with good specificity. This platform has the potential to provide a convenient and efficient tool for the diagnosis of multiple neurological disorders in the future.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Qusai Hassan, Zhixin Meng, Meissam Noroozifar, Kagan Kerman
Summary: Sugarcane biochar and methylene blue were used to develop a graphite paste electrode (GPE-SC-MB) for the simultaneous detection of four DNA bases. The linear ranges and detection limits were as follows: G: 0.67-38.67 μM and 0.037 μM, A: 0.67-126.67 μM and 0.042 μM, and T/C: 6.67-1600 μM and 4.25 μM, 5.33 μM. The GPE-SC-MB showed recovery values between 99.0 and 103.0% when tested in human saliva, indicating the effectiveness of sugarcane biochar as an electrode modifier for sensitive electrochemical sensors.
Article
Chemistry, Analytical
Rokhsareh Abedi, Jahan Bakhsh Raoof, Mojtaba Mohseni, Ayemeh Bagheri Hashkavayi
Summary: An electrochemical aptasensor has been developed for the detection of A. baumannii by modifying carbon screen-printed electrodes with a synthesized nanocomposite and binding A. baumannii-specific aptamer. The aptasensor showed high sensitivity, stability, reproducibility, and specificity.
Review
Chemistry, Applied
Songjiao Li, Peipei Wang, Ke Yang, Ying Liu, Dan Cheng, Longwei He
Summary: A near-infrared methylene blue-based fluorogenic probe with on/off activation ability is crucial for detecting and monitoring biological analytes within cells and organisms. By using biomarker-activated probes, precise results can be obtained with minimal photodamage and biological interferences. This review highlights the recent advancements in activatable methylene blue-based fluorogenic probes, focusing on strategies for detecting and imaging disease-related biomarkers and the potential challenges and opportunities in developing new methods to detect and image biomarkers.
Article
Electrochemistry
Fernando Otero, Kim Shortall, Urszula Salaj-Kosla, Syed A. M. Tofail, Edmond Magner
Summary: An electrochemical biosensor was developed using a hairpin DNA probe labeled with methylene blue for detecting the TP53 gene. The biosensor could selectively detect a single nucleotide polymorphism in the TP53 gene affecting residue 175 of the p53 protein, with a more pronounced response at shorter hybridization times.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ziqiao Yan, Minyi He, Yuwei Zhang, Gengxin Hu, Hong Li
Summary: The study demonstrates that methylene blue can significantly amplify the oxidation signals of tyrosine residues in proteins. By evaluating various factors, the mechanism of signal amplification is determined. This method of signal enhancement shows good selectivity, high reproducibility, and strong anti-interference ability.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dong Liu, Shuyun Meng, Xiuli Shen, Yuye Li, Xiaohong Yan, Tianyan You
Summary: This work presents a dual-ratiometric aptasensor for streptomycin detection, utilizing the in-situ coupling of photoelectrochemical and electrochemical assays. The use of methylene blue as a bifunctional probe enables the simultaneous generation of photocurrent and redox current, resulting in enhanced analytical performances compared to single-signal or single-ratiometric assays. The MB-based dual-mode sensing offers a promising tool for studying interactions between PEC and EC behaviors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Urvasini Singh, Sunita Kumbhat
Summary: The electrochemical sensor proposed is capable of rapidly detecting and selectively identifying lithium ions in solution, holding promise for point-of-care lithium level monitoring in clinical and biomedical fields.
Article
Chemistry, Physical
Mazhar Hayat, Afzal Shah, Jan Nisar, Iltaf Shah, Abdul Haleem, Muhammad Naeem Ashiq
Summary: An electrochemical sensor was developed for the detection of methylene blue in aqueous solutions, and for monitoring its degradation process. The sensor showed sensitivity, selectivity, stability, and rapid responsiveness, and was capable of analyzing small sample volumes.
Article
Environmental Sciences
Linlin Wang, Liang Wang, Yawei Shi, Bin Zhao, Zhaohui Zhang, Guanghui Ding, Hongwei Zhang
Summary: In this study, a Ti3+-doped TiO2 porous membrane was successfully prepared and used for electrochemical degradation of organic pollutants. The Blue TiO2/Ti membrane exhibited higher activity and better removal efficiency compared to other membranes. The degradation pathways of pollutants were proposed by analyzing the oxidation products. Additionally, the long-term operation performance of the Blue TiO2/Ti membrane was evaluated.
Article
Environmental Sciences
Zhenyu Hu, Chao Guo, Peng Wang, Rui Guo, Xuanwen Liu, Ye Tian
Summary: In this study, a SnO2 electrode with a micron-sized sphere structure was prepared by in-situ hydrothermal method, and the Pb-10% electrode exhibited excellent electrooxidation properties, with small charge transfer resistance and high oxygen evolution potential.
Article
Chemistry, Analytical
Fengqin Li, Zhigang Yu, Yanmei Xu, Huiyuan Ma, Guiling Zhang, Yongbin Song, Hong Yan, Xunjun He
ANALYTICA CHIMICA ACTA
(2015)
Article
Biophysics
Fengqin Li, Zhigang Yu, Haichao Qu, Guiling Zhang, Hong Yan, Xin Liu, Xunjun He
BIOSENSORS & BIOELECTRONICS
(2015)
Article
Biophysics
Fengqin Li, Yanmei Xu, Xiang Yu, Zhigang Yu, Xunjun He, Hongrui Ji, Jinghao Dong, Yongbin Song, Hong Yan, Guiling Zhang
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Chemistry, Analytical
Fengqin Li, Yanmei Xu, Xiang Yu, Zhigang Yu, Hongrui Ji, Yongbin Song, Hong Yan, Guiling Zhang
SENSORS AND ACTUATORS B-CHEMICAL
(2016)
Article
Chemistry, Multidisciplinary
Peter M. Wilson, Adam Zobel, Anita J. Zaitouna, Alexey Lipatov, Eva Schubert, Tino Hofmann, Mathias Schubert, Rebecca Lai, Alexander Sinitskii
Article
Chemistry, Analytical
Yanmei Xu, Fengqin Li, Zhigang Yu, Guiling Zhang, Yongbin Song, Hong Yan
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Microbiology
Jie Geng, Anita J. Zaitouna, Malini Raghavan
Article
Biology
Brogan Yarzabek, Anita J. Zaitouna, Eli Olson, Gayathri N. Silva, Jie Geng, Aviva Geretz, Rasmi Thomas, Sujatha Krishnakumar, Daniel S. Ramon, Malini Raghavan
Review
Chemistry, Analytical
Fengqin Li, Zhigang Yu, Xianda Han, Rebecca Y. Lai
ANALYTICA CHIMICA ACTA
(2019)
Article
Materials Science, Multidisciplinary
Huijun Wang, Xinya Jiang, Xia Yang, Zhigang Yu, Ruo Yuan
MATERIALS CHEMISTRY AND PHYSICS
(2019)
Article
Biochemistry & Molecular Biology
Xianda Han, Zhigang Yu, Fengqin Li, Wenbing Shi, Cuicui Fu, Hong Yan, Guiling Zhang
BIOELECTROCHEMISTRY
(2019)
Article
Biochemical Research Methods
Zhigang Yu, Xianda Han, Fengqin Li, Xiaoping Tan, Wenbing Shi, Cuicui Fu, Hong Yan, Guiling Zhang
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Yangkejia Xiang, Zhigang Yu, Dan Liu, Xianda Han, Guiling Zhang
Summary: This study investigated the binding mode of aptamer to ampicillin and its electrochemical response behavior using molecular dynamics simulation. The results showed two different binding modes in different solution systems, which influenced the electrochemical response characteristics of the sensor. The study also found that H2O (0.1 M NaCl) was more conducive to forming a stable aptamer/AMP complex and developing a high-sensitivity analytical method with a low detection limit.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Xianda Han, Zhigang Yu, Yangkejia Xiang, Fengqin Li, Xiaoping Tan, Lan Xu, Guiling Zhang
Summary: An electrochemical fabrication method of Ru[bpy](3)(2+)@Cu-MOF(-SH)/Au was developed, and the proposed interface was characterized using photo/electrochemical information and validated with a DNA-based biosensing assay. The fabricated interface showed a dual signal response in both photochemistry and electrochemistry. The effects of parameter variables on the interface response were investigated, and the results followed an interference effect mechanism. Under optimized conditions, the interface exhibited high sensitivity with a low detection limit. The correlation between the interface fabrication and response performance was validated using both electrochemical and electrochemiluminescence imaging methods.
APPLIED SURFACE SCIENCE
(2022)
Article
Biophysics
Xiang Yu, Zhigang Yu, Fengqin Li, Yanmei Xu, Xunjun He, Lan Xu, Wenbing Shi, Guiling Zhang, Hong Yan
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)