Journal
ANALYTICA CHIMICA ACTA
Volume 759, Issue -, Pages 53-60Publisher
ELSEVIER
DOI: 10.1016/j.aca.2012.10.041
Keywords
Apoferritin; Thrombin; Horseradish peroxidase; Electrochemical aptasensor
Categories
Funding
- National Natural Science Foundation of China [20975037, 21075037, 21275051]
- NIH grant from the National Institute of Environmental Health Sciences (NIEHS) [U54 ES16015]
- DOE [DE-AC05-76RL01830]
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A novel and ultrasensitive sandwich-type electrochemical aptasensor has been developed for the detection of thrombin, based on dual signal-amplification using HRP and apoferritin. Core/shell Fe3O4/Au magnetic nanoparticles (AuMNPs) loading aptamer1 (Apt1) was used as recognition elements, and apoferritin dually labeled with Aptamer2 (Apt2) and HRP was used as a detection probe. Sandwich-type complex, Apt1/thrombin/Apt2-apoferritin NPs-HRP was formed by the affinity reactions between AuMNPs-Apt1, thrombin, and Apt2-apoferritin-HRP. The complex was anchored on a screen-printed carbon electrode (SPCE). Differential pulse voltammetry (DPV) was used to monitor the electrode response. The proposed aptasensor yielded a linear current response to thrombin concentrations over a broad range of 0.5-100 pM with a detection limit of 0.07 pM (S/N = 3). The detection signal was amplified by using apoferritin and HRP. This nanoparticle-based aptasensor offers a new method for rapid, sensitive, selective, and inexpensive quantification of thrombin, and offers a promising potential in protein detection and disease diagnosis. (C) 2012 Elsevier B.V. All rights reserved.
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