Journal
ANALYTICA CHIMICA ACTA
Volume 788, Issue -, Pages 177-182Publisher
ELSEVIER
DOI: 10.1016/j.aca.2013.06.038
Keywords
Rhodamine B; Fluorescent probe; Intracellular pH; Living cells; Lysosomes
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Funding
- 973 Program [2010CB933504]
- National Natural Science Foundation of China [20972088, 90813022]
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We designed and synthesized a new pH fluorescent probe, RCE, based on structural changes of rhodamine dye at different pH values. The probe exhibits high selectivity, high sensitivity and quick response to acidic pH, as well as low cytotoxicity, excellent photostability, reversibility and cell membrane permeability. Fluorescence intensity at 584 nm was increased more than 150-fold within pH range 7.51-3.53. This probe has pK(a) value 4.71, which is valuable for studying acidic organelles. Because of its long absorption and emission wavelengths, RCE can avoid associated cell damage. The probe can selectively stain lysosomes and monitor lysosomal pH changes in living cells. (C) 2013 Elsevier B.V. All rights reserved.
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