Article
Chemistry, Physical
Yuyuan Zhang, Chen He, Kimberly de la Harpe, Peter M. Goodwin, Jeffrey T. Petty, Bern Kohler
Summary: The specific nucleobase subunits within a DNA strand modulate cluster-ligand interactions and brightness, with guanosine and inosine affecting the fluorescence brightness and decay rate differently. Nonradiative decay is 7x higher with guanosine than inosine, indicating a potential correlation between nonradiative decay and selective coordination with specific nucleobase subunits.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Spectroscopy
Amir Amiri Sadeghan, Hossein Soltaninejad, Mehdi Dadmehr, Amir Ali Hamidieh, Mohammad Ali Asadollahi, Morteza Hosseini, Mohammad Reza Ganjali, Saman Hosseinkhani
Summary: This method utilizes a fluorescence turn-on strategy to detect the methylation state of DNA, eliminating the need for labor-intensive chemical or enzymatic treatments. It shows a linear relationship within the detection limit range, exhibits quenching effect on unmethylated DNA, and has high specificity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Juan Fernando Garcia, David Reguera, Andrea Valls, Anna Avino, Arnau Dominguez, Ramon Eritja, Raimundo Gargallo
Summary: This study investigates the use of DNA-stabilized fluorescent silver nanoclusters for the detection of pyrimidine-rich DNA sequences through the formation of parallel and antiparallel triplex structures, using molecular fluorescence spectroscopy. The effectiveness of the formation of triplex structures was evaluated using polyacrylamide gel electrophoresis, circular dichroism, molecular fluorescence spectroscopy, and multivariate data analysis. The results indicate that the detection of pyrimidine-rich sequences with acceptable selectivity is possible using the approach based on the formation of antiparallel triplex structures.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Guowen Li, Xiufang Huang, Chifang Peng, Fengxia Sun
Summary: Through optimizing the synthesis of silver nanoclusters (Ag NCs) using DNA templates, it was found that the fluorescence intensity of T base-extended DNA-templated Ag NCs was more than three times higher than C-riched DNA-templated Ag NCs. A turn-off fluorescence sensor based on the brightest DNA-Ag NCs was constructed for the sensitive detection of dimethoate, ethion, and phorate in lake water samples.
Article
Chemistry, Multidisciplinary
Stacy M. Copp, Anna Gonzalez-Rosell
Summary: DNA-stabilized silver clusters exhibit diverse fluorescence properties based on DNA sequence, with differences in excitation energies and Stokes shifts possibly stemming from variations in cluster structure and DNA conformation. Steady state Stokes shifts of Ag-N-DNAs may provide valuable insights into the optical diversity of these nanoclusters.
Article
Chemistry, Analytical
Zheng Wei Wong, Siu Yee New
Summary: A fluorescence biosensor based on hybridisation chain reaction amplification coupled with silver nanoclusters has been developed for nucleic acid detection, providing a reliable and low risk method for detecting target DNA.
Article
Spectroscopy
Bo Zheng, Binhui Pan, Sheng Xu, Zhihua Xu, Guangrong Lu, FangYan Wang, Biyun Fang, ChangLong Xu
Summary: Research has shown that circulating miRNAs can be used as novel diagnostic and prognostic markers for patients with inflammatory bowel diseases (IBD). In this study, a fluorescent probe was developed for simultaneous detection of two serum biomarkers (miR-23a and miR-223) associated with ulcerative colitis (UC) activity. The method showed high selectivity and has the potential for clinical application.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Nanoscience & Nanotechnology
Qiong Wu, Chengcheng Liu, Yuan Liu, Cheng Cui, Jia Ge, Weihong Tan
Summary: DNA-templated silver nanoclusters (DNA-AgNCs) are promising fluorescent materials for cancer diagnosis. However, their current applications suffer from limited recognition abilities and low detection sensitivity. In this study, the researchers assembled DNA-AgNCs using a multibranched linear (MBL) DNA structure and achieved improved detection sensitivity and logic performance for cancer cell recognition through a combination of cascade strand displacement reaction and aptamer-specific recognition ability.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Yuliang Cheng, Guowen Li, Xiufang Huang, Zhijuan Qian, Chifang Peng
Summary: A label-free fluorescent detection method for glyphosate based on DNA-Ag NCs and a Cu2+-ion-modulated strategy was developed, achieving ultrasensitive detection of glyphosate. The method showed a linear response in the range of 1.0-50 ng/mL with a LOD of 0.2 ng/mL, demonstrating high application potential for glyphosate detection.
Article
Chemistry, Multidisciplinary
Lingyuan Liu, Shuyun Zhu, Jing Sun, Meng Xia, Xian'en Zhao, Guobao Xu
Summary: A ratiometric fluorescent sensing platform for bleomycin (BLM) has been designed using DNA-templated silver nanoclusters (DNA-AgNCs) probe, with a highly-sensitive detection limit of 30 pmol/L. The label-free fluorescence probe offers the advantages of convenient synthetic process and low cost, and has been successfully applied to the detection of BLM in human serum samples, showing potential for cancer therapy analysis.
CHINESE CHEMICAL LETTERS
(2021)
Article
Spectroscopy
Lingyuan Liu, Qianyi Zhang, Feng Li, Mei Wang, Jing Sun, Shuyun Zhu
Summary: Fluorescent DNA-templated silver nanoclusters were synthesized and used as a novel probe for detecting D-penicillamine for the first time, showing high sensitivity and fast preparation process.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Multidisciplinary
Seokjoon Kim, Eun Sung Lee, Byung Seok Cha, Ki Soo Park
Summary: In this study, DNA-templated copper nanoclusters with improved fluorescence stability were prepared by introducing fructose into the synthesis process. The inclusion of fructose significantly increased the operating lifetime of the CuNCs and improved their stability against various conditions. Moreover, the fluorescence signal of CuNCs was maintained for a longer time when stored at low temperatures. These findings provide a basis for improving the stability of copper nanoclusters for various applications.
Article
Chemistry, Multidisciplinary
Yinan Zhang, Zhi-Bei Qu, Chu Jiang, Yingying Liu, Raghu Pradeep Narayanan, Dewight Williams, Xiaolei Zuo, Lihua Wang, Hao Yan, Huajie Liu, Chunhai Fan
Summary: In this research, a new method was developed to fabricate silver nanostructures with three-dimensional chirality using DNA origami-enabled aqueous solution metallization strategy. The study found that the chirality of silver nanostructures can be controlled through synergetic interactions, resulting in the synthesis of helical silver patterns with well-defined chirality and pitches. This method opens up new pathways for synthesizing programmable inorganic materials with arbitrary morphology and chirality.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Applied
Ziyan Yang, Lingling Hu, Keke Ning, Yuan Wu, Jiangong Liang
Summary: In this study, a strong competitive interaction between thiram and silver atoms of DNA-templated silver nanoclusters (DNA-AgNCs) was discovered, resulting in fluorescence quenching of DNA-AgNCs without the need for additional metal ion mediation. This thiram-induced fluorescence quenching phenomenon was used to develop a sensor for thiram detection. The sensor exhibited good linearity and low detection limits for thiram concentration, as well as superior selectivity and practicality in real samples such as apples and soil.
Article
Biochemistry & Molecular Biology
Jinliang Ma, Kexin Li, Shaobin Gu
Summary: This study explores the editing of DNA to regulate and repair protein structure and function. By introducing and moving cytosine, a simple strategy is proposed to modulate the excitation and emission wavelengths of DNA/AgNCs, resulting in nanoclusters with different fluorescence intensity and emissive colors. Ultimately, this research has potential applications in bioimaging and probe design through the manipulation of cytosines in DNA sequences.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)