Journal
ANALYTICA CHIMICA ACTA
Volume 726, Issue -, Pages 67-72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.03.007
Keywords
3,4,9,10-Perylenetetracarboxylic dianhydride; Grapheme sheet; Electrochemiluminescence; Peroxydisulfate; Sandwich-type
Categories
Funding
- NNSF of China [21075100]
- Natural Science Foundation of Chongqing City [CSTC-2009BA1003]
- Ministry of Education of China [708073]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC2010009]
- High Technology Project Foundation of Southwest University [XSGX02]
- Southwest China University [KB2010006]
- Yibin University [2011Z20]
Ask authors/readers for more resources
A novel tracer, 3,4,9,10-perylenetetracarboxylic dianhydride(PTCDA)functionalized graphene sheet (GS) composite (GS-TCDA). is employed to label the secondary anti-thrombin aptamer (TBA) to construct an ultrasensitive electrochemiluminescent sandwich-type aptasensor. The GS provided large surface area for loading abundant PTCDA and TBA with good stability and biocompatibility. Because of the excellent electroconductivity of GS and the desirable optical properties of PTCDA, the as-formed Apt II bioconjugate considerably amplified the electrochmiluminescence (ECL) signal of peroxydisulfate (S2O82-) and worked as the desirable label for Apt II. On the basis of the considerably amplified ECL signal and sandwich format, an extremely wide range from 1 fM to 1 nM with an ultralow detection limit of 0.33 fM for thrombin was obtained. Additionally, the selectivity and stability of the proposed aptasensor were also excellent. Thus, this procedure has great promise for detection of thrombin present at ultra-trace levels during early stage of diseases. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available