Article
Chemistry, Analytical
Luyao Liu, Bin Zhu, Zheng Fang, Na Zhang, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Zhenzhen Yao, Mingliang Ye
Summary: Bottom-up proteomics is increasingly used in clinical research, but glycoproteomic analysis has been a bottleneck. An automated glycopeptide enrichment method was developed for site-specific N-glycoproteome analysis, showing higher efficiency and reliability.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Marcel Macke, C. Derrick Quarles, Michael Sperling, Uwe Karst
Summary: Gadolinium-based contrast agents are commonly used in MRI for improved image contrast, leading to significant environmental release of anthropogenic Gd. This study developed a rapid and efficient monitoring method utilizing a single platform system and ICP-MS for total metal analysis, successfully detecting and quantifying multiple GBCAs in surface water samples.
Article
Chemistry, Analytical
Jun Dai, Chengjie Ji
Summary: Although reversed-phase liquid chromatography (RPLC) is commonly used, other separation modes such as size exclusion chromatography (SEC) and ion-exchange chromatography (IEX) are crucial for characterizing protein therapeutics. This study demonstrates the power of native mass spectrometry (MS) coupled with SEC and IEX for the characterization of bevacizumab and NISTmAb, providing valuable insights into the protein therapeutics at the native state.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Alba Morales-Rodriguez, Miquel Perez-Lopez, Elle Puigpelat, Angels Sahuquillo, Dolores Barron, Jose Fermin Lopez-Sanchez
Summary: This study explored the feasibility of using anionic exchange SPE cartridges for the isolation and preconcentration of arsenosugars, demonstrating successful extraction and analysis of three major arsenosugars with recoveries over 75% for two of them.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Yann Leblanc, Violaine Chapuis, Valegh Faid, Alexander Seifert
Summary: Antithrombin, a key protein in the coagulation system, is a serine protease inhibitor. It is used therapeutically for patients with decreased antithrombin activity. This study presents a method using ion exchange chromatography coupled with mass spectrometry to characterize post-translational modifications of antithrombin and detect inactive conformers.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Kittiya Plaeyao, Ratchaneekorn Kampangta, Yuparat Korkokklang, Chanon Talodthaisong, Apichart Saenchoopa, Saengrawee Thammawithan, Krailikhit Latpala, Rina Patramanon, Navaphun Kayunkid, Sirinan Kulchat
Summary: This study synthesized ginger-stabilized silver nanoparticles (Gin-AgNPs) using an eco-friendly method and demonstrated their application in the detection of Hg2+ in tap water. The colorimetric sensor showed good sensitivity, with a LOD of 1.46 μM and a LOQ of 3.04 μM, and was not affected by other metal ions. Machine learning approach improved the performance of the sensor, achieving accuracy ranging from 0% to 14.66% in detecting different concentrations of Hg2+. Furthermore, the Gin-AgNPs and Gin-AgNPs hydrogels exhibited antibacterial effects, suggesting potential applications in Hg2+ detection and wound healing.
Article
Chemistry, Analytical
Zhenbin Zhang, Norman J. Dovichi
Summary: This study presents an integrated platform that combines a miniaturized filter-aided sample preparation method with high-pH reversed phase or strong cation exchange microreactors for efficient sample preparation and fractionation prior to LC-ESI-MS/MS analysis. The platform generated a large number of unique peptides and protein groups, comparable to state-of-the-art methods, but with reduced cost and time. This integrated platform has significant potential in deep proteomics analysis of mass-limited samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Guillaume Hopsort, Laure Latapie, Karine Groenen Serrano, Karine Loubiere, Theodore Tzedakis
Summary: Analyzing the composition of urine is important in biology and medicine, as it allows for the diagnosis of a person's health condition. A new method combining ion chromatography with mass spectroscopy was introduced to simultaneously determine the major organic molecules and ions in urine. The method showed high accuracy and precision in quantifying the components.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xiaomei Zhou, Woran Song, Milos V. Novotny, Stephen C. Jacobson
Summary: Structural isomers of sialylated N-glycans contribute to the diversity of the N-glycome and can be easily distinguished by mass spectrometry. An improved alkylamidation method with a delactonization step was used to enrich and stabilize sialylated N-glycans for structural characterization.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Chemistry, Analytical
Bastiaan L. Duivelshof, Amarande Murisier, Julien Camperi, Szabolcs Fekete, Alain Beck, Davy Guillarme, Valentina D'Atri
Summary: Fc-fusion proteins are a successful class of biopharmaceutical products with diverse biological ligands, combining tailored pharmacological properties and multiple functions of immunoglobulins. Analyzing Fc-fusion proteins is more complex than monoclonal antibodies due to their highly diverse structures, requiring the development of specific methods for characterization.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Food Science & Technology
Sha Yan, Xuan Wang, Minghui Sun, Wenqian Wang, Liming Wu, Xiaofeng Xue
Summary: Royal jelly is a popular nutraceutical with unique lipid composition, including phospholipids and trans-10-hydroxy-2-decenoic acid. This study found that the lipid composition of royal jelly is influenced by its botanical origin.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Chemistry, Analytical
Adeline Bigard, Pascal Cardinael, Valerie Agasse
Summary: This review discusses recent developments in ion exchange chromatography and mass spectrometry, with a focus on the choice of mobile phases, column geometry, suppressors, make-up solvents, and ionization sources. It also provides an overview of applications in various fields such as food analysis, environmental analysis, and bioanalysis, while discussing performance.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Khageshwar Singh Patel, Piyush Kant Pandey, Pablo Martin-Ramos, Warren T. Corns, Simge Varol, Prosun Bhattacharya, Yanbei Zhu
Summary: Arsenic is a regulated hazard material in the environment, posing risks to humans and causing environmental, agricultural, and health issues. This review comprehensively investigates the sources and mobility of arsenic in various environmental compartments worldwide, as well as measures to address health hazards. Multiple atomic spectrometric approaches have been used for the analysis of arsenic chemical species, with detection limits below 1 μg L-1. Both natural and human activities contribute to arsenic in air, water, soil, and sediment, with reported concentrations often exceeding permissible limits.
Article
Biochemistry & Molecular Biology
Shuang-Qing Zhang, Sui-Han Cheng, Shi Shen, Bi-Ying Luo, Yan Zhang
Summary: A new method was developed for quantifying Cr(III) and Cr(VI) in Chromium-enriched yeast, using ion chromatography-inductively coupled plasma mass spectrometry. Cr(III) and Cr(VI) were extracted using ultrasound-assisted weakly alkaline EDTA solution combined with boiling, and separated on two successive anion-exchange columns. The method was sensitive, accurate, and precise, with no Cr(VI) species found in CrY.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Guusje van Schaick, Nadi el Hajjouti, Simone Nicolardi, Joost den Hartog, Romana Jansen, Rob van der Hoeven, Wim Bijleveld, Nicolas Abello, Manfred Wuhrer, Maurien M. A. Olsthoorn, Elena Dominguez-Vega
Summary: This study developed a workflow for the structural and functional characterization of endo-1,4-beta-xylanase (ENDO-I) and separated proteoforms using mass spectrometry and activity assays. It was found that the glycated species showed lower activity compared to the non-modified enzyme, providing guidance for product development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Thomas Herl, Frank-Michael Matysik
ANALYTICAL CHEMISTRY
(2020)
Review
Electrochemistry
Thomas Herl, Frank-Michael Matysik
Article
Chemistry, Analytical
Ivan Jiranek, Jiri Barek, Frank-Michael Matysik
Article
Chemistry, Multidisciplinary
Beate Scherer, Ingo Leonard Kottenstedde, Frank-Michael Matysik
MONATSHEFTE FUR CHEMIE
(2020)
Article
Chemistry, Analytical
Stefan Weigl, Elisabeth Wittmann, Thomas Rueck, Rudolf Bierl, Frank-Michael Matysik
Summary: The study presents a sensitive UV LED photoacoustic setup for detecting gaseous acetone and explores its potential for breath analysis. Performance of the sensor for low acetone concentrations was investigated, and various factors influencing the measured signal were examined. Cross-sensitivities towards O-2, CO2 and H2O were extensively studied as well. The sensor demonstrated high potential for acetone detection in human breath analysis, with a limit of detection of 12.5 ppbV under typical breath exhale gas mixture conditions.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Correction
Chemistry, Analytical
Stefan Weigl, Elisabeth Wittmann, Thomas Rueck, Rudolf Bierl, Frank-Michael Matysik
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Stefan Wert, Heiko Iken, Michael J. Schoening, Frank-Michael Matysik
Summary: The study investigated the impact of temperature pulses on glucose oxidase (GOx) in glucose biosensors, finding that short heat pulses led to less thermal inactivation of immobilized GOx and increased sensitivity by up to 22-fold.
Article
Nanoscience & Nanotechnology
Christian Iffelsberger, Stefan Wert, Frank-Michael Matysik, Martin Pumera
Summary: Flow reactors play a crucial role in chemical synthesis, electrochemical reactions, and wastewater treatment, with catalyst activity spatial analysis being important for optimization. The use of scanning electrochemical microscopy allows for localized tracking of catalytic activity with high resolution.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Daniel Boehm, Frank-Michael Matysik
Summary: By assembling capillaries with different inner diameters, higher theoretical plate numbers and increased sensitivity can be achieved in capillary electrophoresis, without introducing significant peak tailing.
MONATSHEFTE FUR CHEMIE
(2021)
Article
Biochemical Research Methods
Daniel Boehm, Martin Koall, Frank-Michael Matysik
Summary: In recent years, dual detection concepts for capillary electrophoresis (CE) with at least one nondestructive detector have been developed. To address the issue of linear arrangement when both detectors are destructive, a concept utilizing flow splitters for split CE stream was developed. The study confirmed that the flow splitters did not significantly affect peak shape, LOD, and allowed the usage of capillaries with different ids.
Article
Chemistry, Analytical
Andrea Hajkova-Strejcova, Michal Augustin, Jiri Barek, Christian Iffelsberger, Frank-Michael Matysik, Vlastimil Vyskocil
Summary: A simple and fast electrochemical DNA biosensor was prepared by adsorbing dsDNA from salmon sperm onto a GCE, and the interaction between selected analytes and dsDNA was investigated using various electrochemical detection techniques.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Stefan Wert, Christian Iffelsberger, Katarina A. Novcic, Frank-Michael Matysik, Martin Pumera
Summary: Layered materials and derived 2D material couples have gained attention for their electrocatalytic properties and potential in energy storage. This study investigates the surface of TiS2 and TiSe2 crystals and finds increased electrochemical activity at the edges, expanding our understanding of their properties and implications for future energy applications.
APPLIED MATERIALS TODAY
(2022)
Article
Acoustics
Max Mueller, Thomas Rueck, Simon Jobst, Jonas Pangerl, Stefan Weigl, Rudolf Bierl, Frank -Michael Matysik
Summary: This study presents a novel algorithm (CoNRad) for calculating photoacoustic signals, which can compute the entire relaxation cascade of arbitrarily complex systems. The applicability of the algorithm is demonstrated by experiments on the influence of different bulk compositions on photoacoustic signals. Additionally, the study analyzes the effect of water on photoacoustic carbon monoxide detection.
Article
Chemistry, Multidisciplinary
Johannes Eidenschink, Seyedehelahe Bagherimetkazini, Frank-Michael Matysik
Summary: This study overcomes the limitations of classical electrochemical techniques in identifying reaction product species by combining electrochemistry with mass spectrometry. By implementing a separation technique, the method's performance is further enhanced. The oxidation behavior of cysteine is investigated using direct electrochemistry-mass spectrometry and electrochemistry-capillary electrophoresis-mass spectrometry. The oxidation products of cysteine are successfully identified.
MONATSHEFTE FUR CHEMIE
(2022)
Article
Biochemical Research Methods
Daniel Bohm, Martin Koall, Frank-Michael Matysik
Summary: Dual detection concepts (DDCs) are popular in analytical chemistry. In this work, a novel DDC for capillary electrophoresis (CE) using an amperometric detector (AD) and a mass spectrometer (MS) was described. The AD exhibited high sensitivity while the MS provided excellent selectivity. The CE flow was split to realize the DDC, and it was successfully characterized using ferrocene derivatives and a nonaqueous background electrolyte.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
