Article
Materials Science, Biomaterials
Thuy P. T. Nguyen, Fanyi Li, Brendan Hung, Vinh Xuan Truong, Helmut Thissen, John S. Forsythe, Jessica E. Frith
Summary: Microgels, microscale particles of hydrogel, are attracting increasing attention for tissue regeneration applications due to their ability to support cell-ECM and cell-cell interactions, as well as their high surface area-to-volume ratio facilitating nutrient and metabolite exchange. This article presents methods for fabricating and modifying microfluidic devices using common laboratory consumables, allowing for the production of microgels. The introduced pipet-based chip offers simplicity, tunability, and versatility, making it easy to assemble in most laboratories for the effective production of cell-laden microgels for tissue engineering applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Zhenyu Zhao, Zhen Wang, Gen Li, Zhengwei Cai, Jiezhou Wu, Lei Wang, Lianfu Deng, Ming Cai, Wenguo Cui
Summary: Microfluidic hydrogel microspheres have been widely studied and used in various industries, with a focus on their applications in the medical field for controlled delivery of cells and drugs. These materials enable efficient encapsulation and control of functionalities, promoting collaboration across disciplines and advancing precision medicine and applied materials.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biochemical Research Methods
Huichao Chai, Yongxiang Feng, Fei Liang, Wenhui Wang
Summary: In this paper, a novel microfluidic device with high-efficiency cell trapping and deterministic release is presented. The device includes capillary valves that can open in a predefined pattern to sequentially trap incoming cells into separate sites. Experiments using MCF-7 cells demonstrated the capability of the device for deterministic single cell trapping and release, showing promise as a useful platform for single cell analysis.
Article
Biotechnology & Applied Microbiology
Tao Zhang, Hong Zhang, Wuping Zhou, Keming Jiang, Cong Liu, Ru Wang, Yuanshuai Zhou, Zhiqiang Zhang, Qian Mei, Wen-Fei Dong, Minxuan Sun, Haiwen Li
Summary: This article presents a simple one-step approach for producing and purifying hydrogel microspheres and highlights their potential applications in automated spheroid and organoid cultures as well as drug screening.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Lili Sun, Tianbao Li, Baojin Zhang, Meng Zhang, Juan Xu, Jinyi Wang
Summary: An off-the-shelf microfluidic device was developed here for the fast and straightforward fabrication of highly uniform multiple-holed hollow particles. The structure, size, morphology, and properties of the particles can be easily adjusted by varying the fabrication conditions, providing a powerful platform for the controllable fabrication of multiple-holed hollow particles. This method offers a unique opportunity for application in various cell-based research studies, including the efficient production of HepG2 3D tumor spheroids with excellent viability and uniformity.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Analytical
Lili Cong, Yu Tian, Zepeng Huo, Weiqing Xu, Chunxi Hou, Wei Shi, Weigang Wang, Chongyang Liang, Shuping Xu
Summary: This study introduces a microfluidic droplet-based fluorescence imaging platform for analyzing single cell-secreted VEGF, exploring cellular physiological clues at the single-cell level. By developing silica nanoparticle-based immunoprobes and conducting immunosandwich assays, the method demonstrates cellular heterogeneity in cytokine secretion.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hamed Shieh, Maryam Saadatmand, Mahnaz Eskandari, Dariush Bastani
Summary: This study explores the on-chip production of microgels using external gelation in microfluidic devices, proposing a novel design and utilizing a shielding oil phase to address challenges. The microfluidic device demonstrated the capability of generating highly monodispersed spherical droplets and alginate microgels effectively.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Ampol Kamnerdsook, Ekachai Juntasaro, Numfon Khemthongcharoen, Mayuree Chanasakulniyom, Witsaroot Sripumkhai, Pattaraluck Pattamang, Chamras Promptmas, Nithi Atthi, Wutthinan Jeamsaksiri
Summary: This paper presents a surface modification method for preparing PDMS microfluidic devices with partially hydrophilic-hydrophobic surfaces to generate double emulsion droplets, achieving high encapsulation efficiency. The key component is a permanent chemical coating using Pluronic surfactant, allowing the surface property of PDMS to be modified.
Article
Engineering, Chemical
Yu Han, Xiaofei Xu, Fengxia Liu, Wei Wei, Zhijun Liu
Summary: A novel device combining a Y-junction and a flow-focusing junction was used to fabricate double-layered microbubbles, microdroplets, and a mixture of microproducts simultaneously. High-speed microscopy and digital imaging technologies were applied to observe the evolution of multiphase flow, and dimensional analysis was used to predict the diameters of the microproducts. The analysis of flow patterns under different operating conditions showed significant impacts on the size, generation frequency, and monodispersity of the microproducts.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Engineering, Biomedical
Jianpei Dong, Jianhua Zhou, Hao Tang, Baiqi Chen, Lu Huang
Summary: We propose a laser-guided programmable hydrogel microstructures construction platform for controllable assembly of multiple cellular spheroids into spatially organized multicellular structures, which can be used for in vitro drug evaluation.
Article
Chemistry, Applied
Laura Gallego-Yerga, Cristina de la Torre, Francesco Sansone, Alessandro Casnati, Carmen Ortiz Mellet, Jose M. Garcia Fernandez, Valentin Cena
Summary: Cyclodextrin-calixarene giant amphiphiles are capable of encapsulating and delivering anticancer drugs to tumor cells with high efficiency, significantly enhancing therapeutic efficacy. The redox-responsive disulfide bridge modification in these amphiphiles allows for efficient cargo release in a tumor microenvironment, leading to potent inhibition of cancer cell proliferation.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Multidisciplinary
Zihan Yang, Zhihang Zhou, Tongxu Si, Zhengdong Zhou, Li Zhou, Y. Rebecca Chin, Liang Zhang, Xinyuan Guan, Mengsu Yang
Summary: Cancer metastasis is a major cause of cancer-related death, and it is characterized by excessive extracellular matrix deposition and increased stiffness in solid tumors. The confined migration of tumor cells in these conditions plays a crucial role in metastasis, but inhibitors specifically targeting this type of migration are rare. In this study, a microfluidic chip was designed to screen for drugs that can effectively inhibit confined migration of cancer cells. By applying this chip, three novel inhibitors of confined migration, MA-5, SB-705498, and diphenyleneiodonium chloride, were discovered in multiple cancer types. The mechanisms of action of these inhibitors were found to be targeting mitochondria, actin polymerization, and cell viability, respectively. Overall, a high-throughput microfluidic platform for screening drugs targeting confined migration was established, and three effective inhibitors were identified.
Article
Chemistry, Multidisciplinary
Taiyu Song, Hui Zhang, Zhiqiang Luo, Luoran Shang, Yuanjin Zhao
Summary: Chemotherapy is hindered by tumor heterogeneity in pancreatic cancer patients. This study introduces a microfluidic encapsulated and integrated primary pancreatic cancer cells platform for biomimetic tumor 3D cultivation and clinical drug evaluation. The results demonstrate its potential in clinical drug evaluation.
Review
Biochemical Research Methods
Cheng-Kun He, Chia-Hsien Hsu
Summary: Microfluidic devices are widely used for single-cell capture and cell-cell interaction analysis, improving drug screening and cell signal transduction. This study reviews various microfluidic multiple- and single-cell capture methods, comparing their advantages and disadvantages, and providing suggestions for their application to multiple-cell capture. The results may serve as a reference for research on microfluidic multiple single-cell coculture technology.
Article
Chemistry, Analytical
Weijun Kong, Hongtao Feng, Xiang Qian, Yizhao Chen, Mengying Deng, Pengfei Zhang, Wen Li, Wenting Bu, Wenchao Xu, Wei Jin, Yuqing Huang, Jun Chu, Shangtao Wu, Yan Chen, Yongfan Men
Summary: In this study, a microfluidic method based on oscillating electric field-induced electrojetting was proposed to fabricate size-controllable fluorescent microspheres embedded with various fluorescent substances. The size of droplets can be adjusted by varying the intensity and frequency of the electric field. The prepared microspheres showed narrow size distribution and intense fluorescence emission.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)