Article
Chemistry, Analytical
Ayako Takemori, Philipp T. Kaulich, Liam Cassidy, Nobuaki Takemori, Andreas Tholey
Summary: The combination of liquid chromatography (LC) and gas-phase separation by field-asymmetric ion mobility spectrometry (FAIMS) is a powerful proteoform separation system for top-down proteomics. In this study, an in-depth top-down proteomics workflow, GeLC-FAIMS-MS, was presented, which utilized a molecular-weight-based proteome fractionation approach using SDS-poly-acrylamide gel electrophoresis. The authors optimized the MS parameters for each gel band and compensating voltage (CV) to improve the identification of proteoforms. They also developed a simple and cost-effective method, combining the PEPPI protocol with an AnExSP method, for stain and SDS removal. Results showed that this method was better suited for low-molecular-weight proteoforms, while a methanol-chloroform-water (MCW)-based protocol showed advantages for higher-molecular-weight proteoforms.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Deseree J. Reid, Tapasyatanu Dash, Zhihan Wang, Craig A. Aspinwall, Michael T. Marty
Summary: Daptomycin is a cyclic lipopeptide antibiotic that interacts with lipid membranes in a non-specific manner. The interaction strength depends on membrane rigidity, with more rigid membranes showing stronger interactions. More fluid membranes may undergo pore formation to expose daptomycin to oxidation. These findings highlight the complementary use of native mass spectrometry, fast photochemical oxidation of peptides, and membrane conductance experiments in studying antibiotic-membrane interactions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jordan L. Tanen, Samuel R. White, Duong Ha, Kaveh Jorabchi
Summary: Elemental analysis of fluorochemicals has gained attention due to increased use of fluorinated compounds. In-plasma ionization has limitations for ICPMS applications, but post-ICP chemical ionization using nanospray has improved F detection sensitivity. This study further investigates post-plasma chemical ionization and demonstrates the impact of excess plasma oxygen and use of reagent ions with different metal centers on F detection. The findings present significant improvements for elemental F analysis, especially for online chromatographic detection.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Materials Science, Ceramics
Takumi Hamaguchi, Ryusuke Nakamura, Kohta Asano, Takeshi Wada, Takeyuki Suzuki
Summary: A thin-film diffusion couple consisting of amorphous iron-boron (a-FeB) alloys with different ratios of stable isotopes of B was prepared using a sputtering technique, and the interdiffusion profiles were obtained by secondary-ion mass spectrometry to evaluate the diffusion coefficient of B in a-FeB at temperatures ranging from 533 K to 653 K. The activation energy and pre-exponential factor for diffusion were determined to be 1.51 eV and 3.0 x 10-8 m2 s-1, respectively. Comparison with reported data showed that boron is the fastest diffusing element in a-FeB, and the activation energy for B diffusion is similar to that for the crystallization of iron in alpha-FeB, suggesting that B diffusion is the rate-determining process.
JOURNAL OF NON-CRYSTALLINE SOLIDS
(2023)
Review
Chemistry, Analytical
Takeshi Furuhashi, Wolfram Weckwerth
Summary: Isomeric compounds play important roles in the human body and drugs with different physiological functions, making their measurement crucial in clinical science. However, measuring isomers is often challenging despite chromatographic separation. Affinity-based sample preparation, such as Immunoprecipitation (IP) and molecularly imprinted polymers (MIP), and the coupling with mass spectrometry have become increasingly important. Additionally, alternative methods including changes in ionization and dissociation energy, ion molecule reactions, and ion mobility mass spectrometry (IMS) have been developed due to the insufficient mass accuracy and resolution to distinguish isomeric compounds. This review discusses recent advancements (mostly 2017-) in mass spectrometry-based isomer analysis methods, highlighting the significance of recent technical advances in IMS, particularly for large molecule isomer analyses.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Guilherme L. Tripodi, Magda M. J. Dekker, Jana Roithova, Lawrence Que
Summary: Reactivity of non-heme iron(IV)-oxo complexes is mainly controlled by the ligands, with complexes featuring tetradentate ligands showing the highest reactivity. Fine-tuning of the reactivity of these complexes can be achieved by attaching additional ligands in solution, with the ArIO oxidant playing a previously unknown role. The reactivity of the complexes correlates with the Fe=O and FeO-H stretching vibrations, with the most reactive complex in the series having the weakest Fe=O bond and forming the strongest FeO-H bond.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Jiyeon Lee, Dahye Im, Yajie Liu, Jing Fang, Xibao Tian, Minsu Kim, Wen-Bin Zhang, Jongcheol Seo
Summary: A technique combining ion mobility spectrometry-mass spectrometry (IMS-MS) and supercharging electrospray ionization (ESI) has been demonstrated to effectively differentiate protein chemical topology. By incorporating as many charges as possible into proteins via supercharging ESI, the protein chains can be largely unfolded and stretched, revealing their hidden chemical topology. Different chemical topologies result in different geometric sizes of the unfolded proteins due to constraints in torsional rotations. The introduction of new topological indices allows for successful differentiation of various protein chemical topologies. This technique provides an easy classification of the topological features of complex proteins, previously concealed by their inherent complexity, and establishes a visual standard for discerning protein topologies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Linlin Sun, Xueliang Liu, Sihui Liu, Xiaofeng Chen, Zheng Li
Summary: The on-chip strategy combining conductive nanosensors with a chaotic gas micromixer enables real-time monitoring of volatile metabolites from urine for accurate diagnosis and classification of urinary tract cancers.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Lu Li, Cuiji Sun, Yaoting Sun, Zhen Dong, Runxin Wu, Xiaoting Sun, Hanbin Zhang, Wenhao Jiang, Yan Zhou, Xufeng Cen, Shang Cai, Hongguang Xia, Yi Zhu, Tiannan Guo, Kiryl D. Piatkevich
Summary: This paper describes a spatially resolved proteomics method called Expansion Proteomics (ProteomEx), which combines tissue expansion with mass spectrometry-based proteomics. The method allows quantitative profiling of proteome variability in mammalian tissues using manual microsampling, without the need for custom equipment. The authors validated the method by analyzing brain, liver, and breast cancer samples, and demonstrated its utility by identifying proteins associated with Alzheimer's disease in a mouse model.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Roderick Corstiaan Abraham Versloot, Patricia Arias-Orozco, Matthijs Jonathan Tadema, Florian Leonardus Rudolfus Lucas, Xinghong Zhao, Siewert J. J. Marrink, Oscar Paul Kuipers, Giovanni Maglia
Summary: Mass spectrometry cannot distinguish between molecules with the same mass-to-charge ratio, but nanopore technology can differentiate diastereomeric and enantiomeric differences in peptides that cannot be observed by conventional MS analysis. The resolution in nanopore analysis is likely due to multiple chiral interactions during peptide transport across the nanopore, similar to chiral chromatography. Nanopore technology also offers possibilities for the rapid and cost-effective analysis of peptides, including those that cannot be easily differentiated by mass spectrometry.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Tsz Hei Fong, Wangpan Shi, Guohui Ruan, Siyi Li, Guanghui Liu, Leyun Yang, Kaibin Wu, Jingxian Fan, Chung Lam Ng, Yafang Hu, Haishan Jiang
Summary: This study retrospectively reviewed medical records and constructed a tuberculostearic acid (TBSA)-combined scoring system based on multiple factors, which showed promising results for predicting and diagnosing tuberculous meningitis (TBM).
Article
Chemistry, Analytical
Jie Zhang, Rongxing Zhou, Yanwen Jin, Nansheng Cheng
Summary: A magnetic immunoassay-based diagnostic platform has been developed for cancer biomarker detection by combining rolling circular amplification with ICP-MS, achieving detection of tumor biomarkers in patient samples for potential clinical diagnosis applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Francesco Arioli, Maria Cristina Gamberini, Radmila Pavlovic, Federica Di Cesare, Susanna Draghi, Giulia Bussei, Francesca Mungiguerra, Alessio Casati, Marco Fidani
Summary: The objective of this research was to develop a LC-MSn method for the determination of free cortisol and its metabolites in human urine. The method was validated for accuracy, precision, linearity, and recovery. It was successfully applied to urine samples from healthy subjects and patients with cortisol-related diseases. Furthermore, the method has potential applications in antidoping control and the study of prednisolone metabolism.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Corinne A. Lutomski, Tarick J. El-Baba, Joshua D. Hinkle, Idlir Liko, Jack L. Bennett, Neha V. Kalmankar, Andrew Dolan, Carla Kirschbaum, Kim Greis, Leonhard H. Urner, Parth Kapoor, Hsin-Yung Yen, Kevin Pagel, Christopher Mullen, John E. P. Syka, Carol V. Robinson
Summary: By using an infrared laser in a high-pressure linear ion trap, researchers were able to remove detergent micelles and obtain good sequence coverage for membrane proteins and their complexes through top-down mass spectrometry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Plant Sciences
Dhananjay Gotarkar, Toshisangba Longkumer, Naoki Yamamoto, Amrit Kaur Nanda, Tamara Iglesias, Lin-Feng Li, Berta Miro, Elisa Blanco Gonzalez, Maria Montes Bayon, Kenneth M. Olsen, Yue-Ie Caroline Hsing, Ajay Kohli
Summary: Drought stress in plants leads to differential expression of genes, including one encoding the guanine deaminase OsGDA1 in rice. OsGDA1 can convert guanine to xanthine, impacting genome methylation in rice. Identification of OsGDA1 fills a critical gap in the SAM to xanthine pathway.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemical Research Methods
M. Estela Del Castillo Busto, Susana Cuello-Nunez, Christian Ward-Deitrich, Tim Morley, Heidi Goenaga-Infante
Summary: This study introduces a novel speciation strategy for determining the main Cu-species in human serum, utilizing anion-exchange high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and a label-free protein quantification approach. The methodology was characterized in terms of selectivity, sensitivity, precision, and robustness, with protein recovery assessed by comparison with species-specific isotope dilution (ID).
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biology
Cristina Sanchez-Gonzalez, Laura Moreno, Pilar Aranda, Maria Montes-Bayon, Juan Llopis, Lorenzo Rivas-Garcia
Summary: The study found that diabetes alters the metabolism of zinc, copper, and manganese, and treatment with vanadium(IV) can reverse the changes in zinc and copper homeostasis caused by hyperglycemia. This effect is possibly mediated by a decrease in DMT1 gene expression.
Article
Biochemistry & Molecular Biology
Alonso Rodriguez Pescador, Lucia Gutierrez Romero, Elisa Blanco-Gonzalez, Maria Montes-Bayon, L. Maria Sierra
Summary: This study investigated the cellular uptake, intracellular dissolution, and biological effects of ultra-small iron hydroxide adipate/tartrate coated nanoparticles in various cell lines. The results showed that these nanoparticles had minimal dissolution after uptake, with DNA damage mainly observed in NER deficient cells. The nanoparticles were found to be a safe alternative for anemia management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Toxicology
John R. Foster, Kharmen Billimoria, M. Estela del Castillo Busto, Stanislav Strekopytov, Heidi Goenaga-Infante, Timothy J. Morley
Summary: This study investigated the effects of daily dosing of a molybdenum compound on rats and observed the accumulation of molybdenum in tissues. Although there was no tissue toxicity, prolonged exposure could potentially have adverse effects on cellular function, emphasizing the need for sensitive monitoring of tissue molybdenum levels to prevent toxicity.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jorge Toledano-Serrabona, Octavi Camps-Font, Diogo Pompeu de Moraes, Mario Corte-Rodriguez, Maria Montes-Bayon, Eduard Valmaseda-Castellon, Cosme Gay-Escoda, M. Angeles Sanchez-Garces
Summary: This study evaluated the accumulation of ions in blood and organs caused by titanium metal particles in a mandibular defect in rats, and described the local reaction of oral tissues to this debris. The results suggest that titanium metal particles might increase the concentration of metal ions in vital organs and induce a foreign body reaction.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Chemistry, Analytical
J. S. F. Pereira, R. Alvarez-Fernandez Garcia, M. Corte-Rodriguez, A. Manteca, J. Bettmer, K. L. LeBlanc, Z. Mester, M. Montes-Bayon
Summary: This study used SELTM-1 as a certified reference material to compare the selenium concentrations in intra- and extracellular regions using different experimental procedures. The sequential procedure involving cell washing and freeze drying provided the most accurate results for quantifying the selenium content in both regions.
Biographical-Item
Biochemical Research Methods
Maria Montes-Bayon, Jose Manuel Costa Fernandez
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Spectroscopy
Iria Rujido-Santos, M. Estela del Castillo Busto, Isabel Abad-Alvaro, Paloma Herbello-Hermelo, Pilar Bermejo-Barrera, Maria Carmen Barciela-Alonso, Heidi Goneaga-Infante, Antonio Moreda-Pineiro
Summary: Single particle inductively coupled plasma mass spectrometry (spICP-MS) is used to assess the concentration and size distribution of silver nanoparticles released from textiles. The releasing procedure using shaking and ultrapure water ensures the stability of silver nanoparticles. Filtration and centrifugation do not affect the concentration and size distribution of the silver nanoparticles. The method has low detection limits and good repeatability.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Roberto Alvarez-Fernandez Garcia, Lucia Gutierrez Romero, Jorg Bettmer, Maria Montes-Bayon
Summary: Single cell elemental (SC) analysis of isogenic cell cultures can be done using inductively coupled plasma (ICP-MS) detection. However, 2D cell cultures are just models to simplify the complexity of real tissue samples. In this study, the researchers demonstrated the capabilities of SC-ICP-MS to analyze single cell suspensions of isolated cells from tissues for the first time. They successfully optimized a cocktail of enzymes for tissue disaggregation and showed that the technique can accurately quantify elemental content and protein expression levels in disaggregated cells.
Article
Biochemistry & Molecular Biology
Lorenzo Rivas-Garcia, Alfonso Lopez-Varela, Jose L. Quiles, Maria Montes-Bayon, Pilar Aranda, Juan Llopis, Cristina Sanchez-Gonzalez
Summary: The present study aimed to evaluate the effect of co-treatment with copper (Cu) and bis(maltolato)oxovanadium(IV) (BMOV) on reducing the toxicity of BMOV. The results showed that co-incubation with both metals effectively reduced the toxicity of BMOV and enhanced its potential therapeutic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jorge Toledano-Serrabona, Diogo Pompeu de Moraes, Sara Gonzalez-Morales, M. Angeles Sanchez-Garces, Jorg Bettmer, Maria Montes-Bayon, Mario Corte-Rodriguez
Summary: This study investigates the presence of Ti, Al, and V metal ions and Ti nanoparticles in rat organs after implantoplasty surgery. The authors developed optimized methods for sample preparation and analysis, using microsampling inserts and enzymatic digestion, to quantify the metal contents. The results show a significant increase in Ti concentrations in various tissues of experimental animals compared to the control group, particularly in the brain and spleen. Additionally, Ti-containing nanoparticles were observed in all tissues analyzed.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2023)
Article
Chemistry, Analytical
Lucia Gutierrez-Romero, Elisa Blanco-Gonzailez, Maria Montes-Bayon
Summary: This study reports the combination of fluorescence-activated cell sorting (FACS) and single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) for the first time. The method is used to evaluate the differences in cellular uptake of ultrasmall iron oxide nanoparticles (FeNPs) loaded with cisplatin (IV) prodrug (FeNPs-Pt(IV)) and cisplatin, and their effects on cell viability. FACS is used to separate different cell populations, which are then analyzed using SC-ICP-MS to determine the intracellular amount of platinum. This combined methodology provides a more accurate understanding of the intracellular drug content and cell death mechanisms associated with different drugs, and opens up possibilities for future single-cell experiments.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Kelly L. LeBlanc, Tantima Kumlung, Andres Suarez Priede, Paramee Kumkrong, Thippaya Junvee, Suladda Deawtong, Jorg Bettmer, Maria Montes-Bayon, Zoltan Mester
Summary: Mushrooms are considered a valuable food source due to their high protein and fiber and low fat content. This study showed that selenium-enriched Lingzhi mushrooms contained low levels of selenium, with selenomethionine being the most abundant form. The mycelium of these mushrooms showed significant selenium accumulation and the presence of selenium nanoparticles, which could have potential medicinal value.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
P. Garcia Cancela, N. Gonzalez Quinonez, M. Corte-Rodriguez, J. Bettmer, A. Manteca, M. Montes-Bayon
Summary: This study investigates the incorporation and storage form of copper ions in Streptomyces and reveals the correlation between copper nanoparticles and antibiotic production, suggesting their role in modulating secondary metabolism and preventing copper toxicity.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
