Article
Nanoscience & Nanotechnology
Zhenlong Jiang, Hui Dong, Yanli Zhou, Yanan Chen, Wanqing Hao, Ziyi Zhang, Yizhao Hao, Lantao Liu, Xiaobing Wang, Maotian Xu
Summary: A ratiometric electrochemical detection platform was constructed to simultaneously determine the levels of uric acid (UA) and xanthine (XA). The cPDA-Gp electrode was prepared by in situ modification of polydopamine (PDA) on graphene paper (Gp) and subsequent carbonization. Methylene blue (MB) was used as an internal reference and coupled with cPDA-Gp. The sensing platform showed promising results for the detection of UA and XA in real samples.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Analytical
Jie Zheng, Min Zhu, Jiao Kong, Zimu Li, Jianming Jiang, Yachao Xi, Fang Li
Summary: A simple microfluidic paper-based analytical device using platinum nanoparticles as peroxidase mimic was developed for simultaneous determination of glucose and uric acid. The multi-layered structure improves color uniformity and intensity, leading to enhanced sensitivity in detection. The device showed satisfactory results in real serum samples and integrated with smartphone for easy and intelligent detection, making it suitable for simple and portable on-site analysis.
Article
Chemistry, Analytical
Fotini Vasiliou, Alexios K. Plessas, Anastasios Economou, Nicolaos Thomaidis, Giannis S. Papaefstathiou, Christos Kokkinos
Summary: In this work, a new type of graphite paste electrode modified with a water-stable Cu(II)-complex was used for the voltammetric determination of glucose and uric acid in sweat. The electrode showed favorable electrochemical performance and low limits of detection for both biomarkers, without interference from common electroactive metabolites.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Li-Xia Feng, Chao Tang, Xiao-Xuan Han, Hui-Chao Zhang, Feng-Na Guo, Ting Yang, Jian-Hua Wang
Summary: In this study, ZnO nanorods were hydrothermally grown on Whatman filter paper to fabricate a microfluidic paper-based device for simultaneous detection of glucose and uric acid. The presence of ZnO NRs promoted enzyme immobilization and overcame the coffee ring effect through uniform distribution and specialized design. Two color indicators provided complementary results for quantifying target analytes visually.
Article
Chemistry, Analytical
Manassawee Janrod, Monpichar Srisa-Art
Summary: A microfluidic paper-based analytical device (mu PAD) with colorimetric detection was developed for simultaneous determination of urinary oxalate, citrate, and uric acid. The detection methods utilized enzymatic reactions and an indicator displacement assay. The mu PAD showed promising results with linear ranges and low detection limits for the three biomarkers, as well as acceptable accuracy and precision in urine sample testing. This development holds great potential for on-site analysis, providing a simple, fast, affordable, and portable tool for simultaneous determination of the biomarkers.
ANALYTICAL METHODS
(2023)
Article
Biophysics
Yiqun Liu, Yaping Dong, Miao Hui, Lingyi Xu, Le Ye, Jicheng Lv, Li Yang, Yue Cui
Summary: This study presents a biosensing array for the multiplex and simultaneous determination of blood glucose, creatinine, and uric acid. The array exhibits high sensitivity and selectivity in real blood samples, with accurate measurement results consistent with those from the clinical biochemistry analyzer.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Yuemeng Cheng, Shaoqing Feng, Qihong Ning, Tangan Li, Hao Xu, Qingwen Sun, Daxiang Cui, Kan Wang
Summary: In this research, a small, convenient, and noninvasive paper-based microfluidic sweat sensor was designed and implemented to detect multiple key biomarkers in human sweat simultaneously. The chip's origami structure includes colorimetric and electrochemical sensing regions. Specific chromogenic reagents are used to selectively identify glucose, lactate, uric acid, magnesium ions, and the pH value in different colorimetric sensing regions. The cortisol in sweat is detected by molecular imprinting in the electrochemical sensing regions. The proposed sweat sensor, composed of hydrophilically and hydrophobically treated filter paper, and 3D microfluidic channels formed by folding paper, has been verified to reliably and noninvasively identify a variety of sweat biomarkers through on-body experiments.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Chemistry, Inorganic & Nuclear
Keke Liu, Yanan Chen, Xueliang Dong, Haiping Huang
Summary: A highly selective and facile sensing platform based on MOF-235 has been developed for the synchronous detection of dopamine and uric acid. The MOF-235 modified electrode exhibits excellent permselectivity and sensitivity, with linear response over a concentration range and low detection limits for both dopamine and uric acid.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Fang Wang, Fengna Shi, Jingfang Li, Naipin Chen, Cheng Chen, Ziqi Xu, Jiajun Wang
Summary: In this study, a novel flexible nonenzymatic glucose and uric acid sensor was developed using carbon cloth loaded with copper microspheres (Cu MSs) and zinc oxide nanoparticles decorated single-walled carbon nanotubes (Cu MSs/ ZnO@CNTs/CC). The nanocomposite electrode exhibited wide linear detection range, low detection limit, outstanding sensitivity, fast response time, and favorable long-term stability. Moreover, the sensor also showed considerable precision and accuracy in practical serum samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Umesha Mogera, Heng Guo, Myeong Namkoong, Md Saifur Rahman, Tan Nguyen, Limei Tian
Summary: Wearable sweat sensors have the potential to provide clinically meaningful information associated with the health and disease states of individuals. Researchers have introduced a wearable plasmonic paper-based microfluidic system that utilizes label-free surface-enhanced Raman spectroscopy (SERS) to detect and quantify metabolites in sweat. This system allows for continuous and simultaneous analysis of sweat loss, sweat rate, and specific biomarkers, providing valuable information for monitoring individual health.
Article
Chemistry, Analytical
Michal Kopcil, Roman Kand'ar
Summary: Uric acid and its oxidation product allantoin serve as excellent biomarkers for oxidative stress in humans. A fast, sensitive, and reliable HPLC-MS/MS method was developed for the simultaneous determination of uric acid and allantoin from dried blood spots. The method utilizes stable isotopically labeled analogs as internal standards and can provide accurate results within a short analysis time. This method is suitable for screening and monitoring allantoin and uric acid levels as biomarkers of oxidative stress in clinical practice.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Asli Neslihan Avan, Sema Demirci-Cekic, Resat Apak
Summary: Immobilization of biological enzymes using magnetic nanoparticles as support materials enables cost reduction and reusability. In this study, three different oxidase enzymes were successfully immobilized on MNPs, and colorimetric nanobiosensors were developed for the determination of biologically important substrates. The immobilized enzymes showed no loss of affinity for the substrate, and the system exhibited wide linear concentration ranges and low detection limits, with the ability to be reused multiple times.
Article
Biophysics
Praveen K. Sekar, Xin M. Liang, Seong-Joong Kahng, Zhiquan Shu, Anthony B. Dichiara, Jae-Hyun Chung, Yanyun Wu, Dayong Gao
Summary: The newly developed carbon nanotube-paper composite capacitance sensor can simultaneously evaluate coagulation function, platelet count or function, and hematocrit, with distinctive sensitivities towards multiple clotting elements. Hemostasis assessments conducted on whole blood demonstrate the potential utility of the sensor for various hemostatic conditions.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Shining Wu, Haoran Wang, Bo Zhao, Tongtong Cao, Juanjuan Ma, Lin Liu, Zhiwei Tong
Summary: A highly sensitive nanocomposite electrochemical sensor for simultaneous detection of dopamine, ascorbic acid, and uric acid was successfully synthesized and characterized in terms of structure and electrochemical properties. The experimental results demonstrate that the sensor has good stability and anti-interference performance, with detection limits for dopamine, ascorbic acid, and uric acid within specific concentration ranges.
Article
Chemistry, Analytical
Ondrej Moravcik, Milos Dvorak, Pavel Kuban
Summary: A new set-up for fully autonomous and high-throughput capillary electrophoresis analysis of dried blood spot samples is presented. The set-up enables unmanned sample processing and analysis with tailor-programmed CE sequences, allowing for a throughput of 240 samples per day. The analytical protocol provides excellent precision and calibration curves with linear ranges and detection limits suitable for clinical purposes. The stability of the samples stored at laboratory temperature for up to 2 months is also demonstrated.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Lu-Ying Duan, Yu-Jie Wang, Jin-Wen Liu, Yu-Min Wang, Na Li, Jian-Hui Jiang
CHEMICAL COMMUNICATIONS
(2018)
Article
Chemistry, Analytical
Lan Liu, Cai-Xia Dou, Jin-Wen Liu, Xiang-Nan Wang, Zhan-Ming Ying, Jian-Hui Jiang
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Analytical
Xueli Zhu, Hongyan Ye, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Analytical
Na Li, Xiu-Yan Wang, Mei-Hao Xiang, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Xueli Zhu, Bin Qu, Zhan-Ming Ying, Jin-Wen Liu, Zhenkun Wu, Ru-Qin Yu, Jian-Hui Jiang
ANALYTICAL CHEMISTRY
(2020)
Article
Biophysics
Lu-Ying Duan, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
Summary: DNA molecular probes are powerful tools for fluorescence imaging of miRNAs in living cells. A novel DNA nanomachine has been developed to amplify sensing and imaging of tumor-related miR-21 with dynamic responses, excellent sensitivity and selectivity. This nanoplatform shows potential for accurate monitoring of miR-21 expression levels for tumor diagnosis and biological research.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Tingting Chen, Yingfeng Qin, Beibei Wang, Rongji Lai, Guohe Tan, Jin-Wen Liu
Summary: A novel fluorescent strategy using enzymatic reaction modulated DNA assembly on graphitic carbon nitride nanosheets (CNNS) has been developed for the detection of acetylcholinesterase (AChE) activity and its inhibitors. The strategy was successfully applied to the assay of AChE in human serum samples and can be used to effectively screen AChE inhibitors, providing a robust and effective platform for AChE-related diagnosis, drug screening, and therapy.
Article
Engineering, Environmental
Lu-Ying Duan, Yan-Ping Hong, Wu-Ying Yang, Lin -Lin Zhang, Jin-Wen Liu
Summary: A DNA-nucleated spherical nucleic acid (DSNA) was developed for sensitive imaging of tumor-related miRNA in living cells. Compared with traditional AuNP-based spherical nucleic acid, the DSNA exhibited superb biocompatibility and stability, excellent cell internalization capability and accessibility, and thus offered a new avenue for early tumor diagnosis and related biomedical research.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Lu-Ying Duan, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
Summary: In this study, we developed a novel nanocomposite material by in situ growing MnO2 nanosheets on the surface of BCNPs through a redox reaction, enabling tumor-specific affinity recognition and two-photon fluorescence activation imaging. The method shows good biocompatibility and highly sensitive and selective responses to GSH, making it a promising tool for biological exploration and clinical diagnosis.
Article
Chemistry, Multidisciplinary
Meng-Mei Lv, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
Article
Chemistry, Multidisciplinary
Mei-Hao Xiang, Na Li, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
Article
Chemistry, Multidisciplinary
Xueli Zhu, Meng-Mei Lv, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Na Li, Lan Liu, Mei-Hao Xiang, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Analytical
Lin Tan, Liu Xu, Jin-Wen Liu, Li-Juan Tang, Hao Tang, Ruqin Yu
ANALYTICAL METHODS
(2019)