Journal
ANALYST
Volume 143, Issue 17, Pages 4067-4073Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8an00987b
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Funding
- National Natural Science Foundation of China [21705045, 21305041, 21475041]
- Natural Science Foundation of Hunan Province [2018JJ2252]
- Scientific Research Fund of Hunan Provincial Education Department [17A125]
- Science and Technology Project of Changsha [KQ1707009]
- Key Project of Research and Development Plan of Hunan Province [2016SK2020]
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Herein, Au nanocluster (AuNC)-embedded chitosan (CS) nanocapsules were prepared and used as a novel signal amplification system for the sensitive fluorescence immunoassay of Escherichia coli O157:H7 (E. coli O157:H7). AuNCs were embedded in CS to form AuNCs@CS nanocapsules, where a large number of AuNCs were embedded in each nanocapsule. A rapid, ultrasensitive, and selective method for the fluorescence detection of E. coli O157:H7 was developed by coupling the nanocapsule-amplification strategy with immunological recognition and magnetic separation. Compared with a conventional immunoassay using AuNCs as labels, the fluorescence signals of the proposed immunoassay were greatly amplified, because much more fluorescent AuNCs are linked to each bacterial cell. As a result, a linear range was obtained from 3 to 700 CFU mL(-1), with a detection limit of 1 CFU mL(-1). This method was successfully used to detect E. coli O157:H7 in drinking water and milk samples.
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