Journal
ANALYST
Volume 139, Issue 16, Pages 4022-4030Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an02347h
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Funding
- CSIR [02(2490)/11/EMR-II]
- DST, New Delhi [SR/S1/IC-20/2012]
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A diformyl-p-cresol (DFC)-8-aminoquinoline based dual signaling probe was found to exhibit colorimetric and fluorogenic properties on selective binding towards Mg2+ and Zn2+. Turn-on fluorescent enhancements (FE) as high as 40 fold and 53 fold in 9 : 1 MeCN/water (v/v) at pH 7.2 in HEPES buffer for Mg2+ and Zn2+, respectively, were observed. The binding constants determined from the fluorescence titration data are: K = (1.52 +/- 0.21) x 10(5) M-1 and (9.34 +/- 4.0) x 10(3) M-2 at n = 1 and 0.5, for Mg2+ and Zn2+, respectively. The L : M binding ratios were also determined by Job's method, which support the above findings. This is further substantiated by HRMS analysis. Due to solubility in mixed organo-aqueous solvents as well as cell permeability it could be used for the in vitro/in vivo cell imaging of Mg2+ and Zn2+ ions with no or negligible cytotoxicity. This probe could be made selective towards Mg2+ over Zn2+ in the presence of TPEN, both under intra- and extracellular conditions and is superior to other Mg2+ probes which suffer from selectivity of Mg2+ over Ca2+ or Zn2+. Furthermore the dissociation constant (K-d = 6.60 mu M) of the Mg2+-(DFC-8-AQ) complex is far lower than the so far reported Mg2+ probes which fall in the mM range.
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