Journal
ANALYST
Volume 139, Issue 5, Pages 987-991Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an02088f
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Funding
- National Natural Science Foundation of China [20875073]
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A unique boronate-functionalized SiO2/TiO2 hybrid monolithic column was synthesized by a facile approach. Although a conventional boronic acid, 4-vinylphenylboronic acid, was used as the affinity ligand, the prepared monolithic column exhibited specific capacity to capture glycoproteins including antibodies in aqueous solution at neutral pH. With the incorporation of titania, the monolith was highly hydrophilic, and the procedure of affinity chromatography could be performed under aqueous organic-solvent-free conditions.
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