Journal
ANALYST
Volume 139, Issue 16, Pages 4016-4021Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an00493k
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japanese Government
- Grants-in-Aid for Scientific Research [25620136] Funding Source: KAKEN
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Here we study the binding behavior of perylenediimide (PDI) derivatives to a hydrophobic pocket created inside DNA and their photochemical properties capable of designing a light-up fluorescent sensor for short single-stranded DNA or RNA. The perylenediimide derivative with alkoxy groups (PO) suppressing electron transfer quenching was examined. The PO bound randomly to DNA showed negligible fluorescence due to the aggregation-induced quenching, whereas the PO bound to the pocket as a monomeric form showed more than 100-fold fluorescence enhancement. Switching the binding states of the PO corresponded to a change in the fluorescence response for the hybridization event, which allowed us to design a fluorescent sensor of nucleic acids with a nanomolar detection limit.
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