Article
Biochemistry & Molecular Biology
Anton M. Solovjev, Ivan I. Galkin, Olga Yu Pletjushkina, Alexey Medvedko, Shulin Zhao, Ivan Yu Sakharov
Summary: A sensitive and specific heterogeneous assay for quantitation of miRNA-39 was developed using ICSDPR, polyperoxidase conjugated with streptavidin, and enhanced chemiluminescence. The assay demonstrated a low detection limit, low coefficient of variation, high specificity, and negligible matrix effect, making it useful for estimating miRNA purification yield from cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Zhang Zhang, Li Zhang, Youqiang Wang, Juan Yao, Ting Wang, Zhi Weng, Liu Yang, Guoming Xie
Summary: This study introduces an ultrasensitive electrochemical miRNA biosensor based on strand displacement reaction circuits and strand displacement polymerization reaction, demonstrating high sensitivity and discriminatory capability towards let 7a for early cancer screening, providing a new research avenue.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Jing Li, Yong-Wei Feng, Li-Jun Huang, Rui Jiang, Xiao-Fang Shen
Summary: Proper labeling of meat products is crucial for consumers who want to avoid specific meat species for cultural or religious reasons. The newly developed SDPICA detection method can identify undeclared pork components in meat products within 20 minutes at 37 degrees Celsius, with a sensitivity as low as 0.1% in multi-component meat mixtures.
Article
Chemistry, Analytical
Shuyi He, Peng Li, Le Tang, Mingjian Chen, Yuxin Yang, Zhaoyang Zeng, Wei Xiong, Xu Wu, Jiahao Huang
Summary: A sensitive fluorescent DNA detection strategy is developed based on a dual-stage amplification process mediated by DNA polymerase. With carefully studied experimental conditions, the method shows linear response to DNA targets from 0.5 to 50.0 nM with a relatively low detection limit.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Yifei Chen, Lizhen Ye, Hui Chen, Tingting Fan, Cheng Qiu, Yan Chen, Yuyang Jiang
Summary: miRNA is a promising biomarker for cancer diagnosis, and the development of new detection methods for it has been a hot topic recently. In this study, we identified miR-625-5p as a potential biomarker for colorectal cancer through expression analysis and phenotypic experiments. We also developed a novel detection method based on strand displacement amplification (SDA) to monitor miR-625-5p levels, which showed good response and high specificity.
Article
Chemistry, Analytical
Changrui Feng, Jiaxin Guo, Gaiping Li, Baoxian Ye, Lina Zou
Summary: A novel simple multiple strand displacement reaction (MSDR) coupled hybridization strand reaction (HCR) was proposed for label-free and ultrasensitive Type b3a2 assay. The method achieves target recycling and amplification by releasing multiple messenger DNAs (mDNA) and forms a linear duplex-DNA structure for in-situ growth of silver nanoparticles (AgNPs) to generate electrochemical response. Under optimal conditions, the biosensor achieved a low detection limit of 0.56 fM and a wide linear range from 10 fM to 10 nM for sensitive detection of Type b3a2 with good selectivity and stability.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Nan Jiang, Peng Li, Shihao Sun, Wei Wei
Summary: A novel ratiometric fluorescence sensor based on strand displacement reaction and magnetic separation was developed for highly sensitive and selective detection of HMF. The sensor has a wide linear range and high selectivity against other interfering substances, making it a potential tool for food safety and environmental monitoring.
Article
Chemistry, Applied
Bing Han, Cheng Fang, Lingjun Sha, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: A new fluorescent aptasensor using cascade strand displacement reaction was proposed for the detection of ochratoxin A, achieving an improved detection limit and short assay time. The method showed potential for use in food quality control in the future.
Article
Biochemistry & Molecular Biology
Dawei Li, Shen Ling, Dudu Meng, Bing Zhou, Pengda Liang, Bei Lv
Summary: An ultrasensitive biosensor was developed for the detection of tobramycin, which exhibited high selectivity and could be applied in food safety control and environmental monitoring.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Multidisciplinary
Xiaobo Zhang, Zhiru Li, Lin Hong, Xiuwen Wang, Jijuan Cao
Summary: In this study, a paper-based electrochemical aptasensor was designed, utilizing TDNs technology to improve the recognition efficiency of the aptamer towards FB1. The aptasensor showed sensitivity and reliability in the analysis of FB1 at low concentrations, demonstrating its great potential in food safety and public health.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wanling Cui, Guodong Hu, Feng Song, Rui Wang, Zanxia Cao, Junye Zhang, Tiejun Wang, Fanlu Meng, Congcong Shen, Shicai Xu, Jihua Wang
Summary: DNA methyltransferase (MTase) is considered a promising biomarker and therapeutic target, playing a crucial regulatory role in various biological processes. A cascade strand displacement amplification strategy was developed for highly sensitive and label-free fluorescent detection of DNA MTase activity, showing improved detection sensitivity. The strategy also effectively evaluated the inhibitory effects of 5-fluorouracil, benzylpenicillin, and gentamycin on DNA MTase activity, suggesting great potential for new method exploration and early disease diagnosis.
MICROCHEMICAL JOURNAL
(2021)
Article
Biophysics
Xinmei Song, Cuiyun Yang, Ruo Yuan, Yun Xiang
Summary: In this study, we demonstrate the construction of several new and label-free electrochemical logic gates by designing G-quadruplexes with Y junction structures. Different combinations of input molecules can alter the Y junction structures and disrupt the formation of G-quadruplexes, allowing for the successful function of different logic operations.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xue Chen, Jialiang Wu, Dengfeng Qu, Shuang Li, Li Wang, Fang Li, Shufeng Liu
Summary: A DNA polymerase-powered self-propelled DNA walking strategy was developed to achieve one-step, dual-signal and amplified nucleic acid detection, providing enormous opportunities for clinical biomedicine and disease diagnosis.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Ye Chen, Ruru Gao, Ruoyang Ji, Wei Dong
Summary: MicroRNA is a vital biomarker due to its abnormal expression in diseases, particularly cancers. A label-free fluorescent sensing platform is proposed for detecting microRNA-21, utilizing the cascade toehold-mediated strand displacement reaction and magnetic beads. The method exhibits a wide linear range and low limits of detection, and possesses great specificity and reliability for distinguishing microRNA-21 from other cancer-related microRNAs. With its fabulous sensitivity, high selectivity, and simplicity of operation, this method offers a promising approach for microRNA-21 detection in cancer diagnosis and biological research.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Yuqi Lu, Feifei Luo, Zhi Li, Ge Dai, Zhaohui Chu, Jingwen Zhang, Fan Zhang, Qingjiang Wang, Pingang He
Summary: A method integrating ISDPR with MCE was developed for rapid and sensitive detection of the mecA gene in methicillin-resistant Staphylococcus aureus strains. The method showed advantages such as label-free detection, ultra-sensitivity, rapid detection, and efficient separation of amplified products.
