Article
Biochemistry & Molecular Biology
Mohamed Y. Zaky, Rehab Mahmoud, Ahmed A. Farghali, Hany Abd El-Raheem, Ahmed Hassaballa, Mohamed Mohany, Dalal Hussien M. Alkhalifah, Wael N. Hozzein, Abdelrahman Mohamed
Summary: This study successfully synthesized and characterized a novel Cu/Fe layer double hydroxide (LDH) nanocomposite for the treatment of prostate cancer. The results demonstrated that Cu/Fe LDH had significant anticancer activities against prostate cancer cells, reducing cell viability, inhibiting cell migration, inducing cell cycle arrest, and triggering apoptosis. It also showed good safety impact on normal cells.
Article
Pharmacology & Pharmacy
Vancha Harish, Devesh Tewari, Sharfuddin Mohd, Pilli Govindaiah, Malakapogu Ravindra Babu, Rajesh Kumar, Monica Gulati, Kuppusamy Gowthamarajan, SubbaRao Madhunapantula, Dinesh Kumar Chellappan, Gaurav Gupta, Kamal Dua, Siva Dallavalasa, Sachin Kumar Singh
Summary: This study focuses on improving the poor oral bioavailability and weak pharmacokinetic profile of Xanthohumol (XH) by developing Xanthohumol-loaded solid lipid nanoparticles (XH-SLNs). The optimized XH-SLNs showed improved bioavailability and sustained release, demonstrating good anticancer properties against PC-3 cells.
Review
Biochemistry & Molecular Biology
Gabriele Brosolo, Andrea Da Porto, Stefano Marcante, Alessandro Picci, Filippo Capilupi, Patrizio Capilupi, Nicole Bertin, Cinzia Vivarelli, Luca Bulfone, Antonio Vacca, Cristiana Catena, Leonardo A. Sechi
Summary: Omega-3 polyunsaturated fatty acids (?-3 PUFAs) have a protective effect on cardiovascular diseases by regulating blood pressure and protecting the vascular system. This narrative review summarizes the results of experimental and clinical studies, highlighting the mechanisms of ?-3 PUFAs on blood pressure and their potential impact on hypertension, hypertension-related vascular damage, and cardiovascular outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Kolodziej, Karolina Chrabaszcz, Ewa Pieta, Natalia Piergies, Julia Rudnicka-Czerwiec, Halina Bartosik-Psujek, Czeslawa Paluszkiewicz, Marian Cholewa, Wojciech M. Kwiatek
Summary: Multiple sclerosis (MS) is a chronic neurodegenerative disease that affects the central nervous system. The diagnosis of MS is crucial for successful treatment, and it typically involves clinical observation, MRI, and analysis of cerebrospinal fluid (CSF). In this study, the researchers propose using spectroscopy as an alternative diagnostic tool, specifically Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy. By analyzing blood plasma and serum samples from MS patients and healthy individuals, the researchers identified specific spectral patterns that can differentiate between MS samples and non-MS samples.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Benan Inan, Betul Mutlu, Guelcan Aysin Karaca, Rabia Cakir Koc, Didem Ozcimen
Summary: This study aimed to evaluate the cytotoxic activity of polar microalgae extracts against different cancer cell lines. The extracts of Chlorella pyrenoidosa showed higher cytotoxicity than Chlorella variabilis against AGS and PC-3 cells. The nanoparticle form of Chlorella variabilis extracts exhibited higher cytotoxic activity and better anticancer properties. These findings provide a foundation for the development of biotechnological drugs based on polar microalgae.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Review
Medicine, Research & Experimental
Nasreddine El Omari, Saad Bakrim, Asaad Khalid, Mohammed Albratty, Ashraf N. Abdalla, Learn-Han Lee, Khang Wen Goh, Long Chiau Ming, Abdelhakim Bouyahya
Summary: Cancer progression is influenced by epigenetic events, with histone modification playing a key role in gene expression. Belinostat, a pan-HDAC inhibitor, has shown effectiveness in the treatment of T-cell lymphoma and solid malignancies. By indirectly promoting acetylated histone accumulation and restoring normal gene expressions, belinostat exhibits potential anti-cancer therapeutic effects through various pathways. Additionally, it has been found to increase p21WAF1, a cyclin-dependent kinase inhibitor involved in cell cycle arrest and apoptosis. Investigative trials have supported belinostat's potential as a valuable anti-cancer drug.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Pharmacology & Pharmacy
Kai Yuan, Xiao Wang, Haojie Dong, Wenjian Min, Haiping Hao, Peng Yang
Summary: CDK4/6 plays crucial roles in regulating the cell cycle and cancer cells, and selectively inhibiting CDK4/6 is an important strategy for cancer treatment. Several highly selective CDK4/6 inhibitors are currently in clinical trials, demonstrating a balance between anticancer efficacy and toxicity.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Engineering, Biomedical
Deborah Mattinzoli, Michele Cacioppo, Masami Ikehata, Silvia Armelloni, Carlo Maria Al, Giuseppe Castellano, Mario Barilani, Francesca Arcudi, Piergiorgio Messa, Maurizio Prato
Summary: This study demonstrates the use of carbon dots (CDs) to enhance the therapeutic effect of SN38 in colorectal cancer (CRC) cells. The covalent conjugation of SN38 to CDs provides a slow, sustained, and pH-responsive drug release, leading to penetration into CRC cells and affecting cellular processes.
MATERIALS TODAY BIO
(2022)
Article
Biochemistry & Molecular Biology
Dongkyu Jeon, Minjae Jo, Yechan Lee, So-Hyeon Park, Hong Thi Lam Phan, Joo Hyun Nam, Wan Namkung
Summary: ANO1, a calcium-activated chloride channel, plays a role in the proliferation, migration, and invasion of various cancer cells. In this study, the effects of two stereoisomers of resveratrol on ANO1 activity were investigated, and it was found that cis- and trans-resveratrol inhibited ANO1 activity with different potencies. Cis-resveratrol was shown to be a potent inhibitor of ANO1 and exhibited ANO1-dependent anticancer activity against human metastatic prostate cancer PC-3 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Daniel Wessling, Simon Maennlin, Ricarda Schwarz, Florian Hagen, Andreas Brendlin, Susann-Cathrin Olthof, Valerie Hattermann, Sebastian Gassenmaier, Judith Herrmann, Heike Preibsch
Summary: This study aims to evaluate the impact of the digital mammography imaging system on overall background enhancement on recombined contrast-enhanced spectral mammography (CESM) images. A retrospective single-center study was conducted on 129 female patients who underwent CESM, and the images were analyzed by two radiologists. The results showed significant differences in qualitative and quantitative background enhancement between different mammography systems, suggesting a potential impact on diagnostic accuracy.
EUROPEAN RADIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Qing Zhang, Wen Wen Chen, Xue Sun, Die Qian, Dan Dan Tang, Li Lin Zhang, Mei Yan Li, Lin Yu Wang, Chun-Jie Wu, Wei Peng
Summary: Cancers are the leading cause of death globally, and emodin, a natural compound derived from plants, has shown reliable anticancer effects on various types of cancer. This review summarizes the sources, anticancer properties, molecular mechanisms, and toxicities of emodin, while also discussing the limitations and future prospects of its research.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Hengmin Han, Seon-Ok Lee, Yinzhu Xu, Jung-Eun Kim, Hyo-Jeong Lee
Summary: Chrysin inhibits tumor progression in hypoxic prostate cancer cells by reducing angiogenesis and promoting apoptosis. This compound exerts its anti-tumor effects by inhibiting the SPHK-1/HIF-1 alpha signaling pathway.
Article
Biochemistry & Molecular Biology
Sonia Di Gaetano, Luciano Pirone, Ioannis Galdadas, Serena Traboni, Alfonso Iadonisi, Emilia Pedone, Michele Saviano, Francesco Luigi Gervasio, Domenica Capasso
Summary: Galectins are soluble proteins involved in cancer progression and disease outcome, making them important targets for therapeutic intervention. Developing small inhibitors to selectively block galectin activity is a promising strategy for cancer therapy. This study designed a novel compound with high binding affinity to galectins and demonstrated its potential anticancer properties, paving the way for further development as an anticancer agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Baris Ata, Antonio La Marca, Nikolaos Polyzos
Summary: Ovarian stimulation for assisted reproductive technology traditionally starts in the early follicular phase, but recent research suggests that the timing of stimulation does not affect the number or quality of oocytes collected. This provides an opportunity for more flexibility in treatment. Random start cycles, where ovarian stimulation begins at any point in the menstrual cycle, have shown promise in improving individualized care for patients.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Immunology
Hung-Ling Huang, Jung-Yu Lee, Yu-Shu Lo, I-Hsin Liu, Sing-Han Huang, Yu-Wei Huang, Meng-Rui Lee, Chih-Hsin Lee, Meng-Hsuan Cheng, Po-Liang Lu, Jann-Yuan Wang, Jinn-Moon Yang, Inn-Wen Chong
Summary: This study successfully developed a predictive model for rifapentine-related systemic drug reactions using whole-blood transcriptomic data and machine learning methods, which performed well in different subgroups. It has important implications for establishing a safe and personalized program for latent tuberculosis infection.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Plant Sciences
Maxime Scheepers, Julien Spielmann, Madeleine Boulanger, Monique Carnol, Bernard Bosman, Edwin De Pauw, Erik Goormaghtigh, Patrick Motte, Marc Hanikenne
Article
Chemistry, Analytical
Allison Derenne, Kheiro-Mouna Derfoufi, Ben Cowper, Cedric Delporte, Erik Goormaghtigh
ANALYTICA CHIMICA ACTA
(2020)
Article
Biochemical Research Methods
Maxime Lobry, Mederic Loyez, Karima Chah, Eman M. Hassan, Erik Goormaghtigh, Maria C. DeRosa, Ruddy Wattiez, Christophe Caucheteur
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Chemistry, Analytical
Joelle De Meutter, Erik Goormaghtigh
Summary: Defining protein secondary structure content can impact prediction quality significantly, with variations observed in secondary structure content based on different definitions. The DSSP algorithm is a good choice for evaluating protein secondary structure content when dealing with FTIR spectra, and selecting an adequate set of definitions can improve prediction quality by 20-50%.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Barbara Claro, Eva Gonzalez-Freire, Martin Calvelo, Lucinda J. Bessa, Erik Goormaghtigh, Manuel Amorin, Juan R. Granja, Rebeca Garcia-Fandino, Margarida Bastos
COLLOIDS AND SURFACES B-BIOINTERFACES
(2020)
Article
Chemistry, Analytical
Joelle De Meutter, Erik Goormaghtigh
Summary: The paper presents a new method for high-throughput protein structure determination using protein microarrays and FTIR imaging. It introduces a new protein library cSP92 designed to cover a wide range of structural space, and correlates spectral characteristics to secondary structure features using ASLR, PLS, and SVM. The results show that ASLR provides better results and the high throughput microarray approach is suitable for accurately determining protein secondary structure.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Joelle De Meutter, Erik Goormaghtigh
Summary: FTIR spectroscopy is a major tool for determining protein secondary structure, but the overlap of absorption bands presents a challenge. Deuteration can improve predictions for alpha-helical and other structures, with beta-sheet content better predicted in non-deuterated conditions.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biophysics
Joelle De Meutter, Erik Goormaghtigh
Summary: The study evaluates the contribution of amino acid side chains in the spectral region and the potential improvement of protein secondary structure prediction after correcting for their contribution. It shows that the prediction of beta-sheet content is significantly improved by subtracting amino acid side chain contributions in the amide I-amide II spectral range. Other methods such as partial least square regression and support vector machine also show improved accuracy for beta-sheet content evaluation.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biophysics
Barbara Claro, Erik Goormaghtigh, Margarida Bastos
Summary: The study successfully characterized the structural features of two cyclic peptides in solution and upon interaction with negatively charged membranes using ATR-FTIR and polarized light, demonstrating that the presence of lipid membranes enhances the formation and orientation of tubular structures.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Chemistry, Analytical
Sabrina Hamla, Pierre-Yves Sacre, Allison Derenne, Kheiro-Mouna Derfoufi, Ben Cowper, Claire Butre, Arnaud Delobel, Erik Goormaghtigh, Philippe Hubert, Eric Ziemons
Summary: Glycosylation plays a critical role in the stability, bioactivity, and safety of pharmaceutical proteins. The development of fast and simple analytical methods is crucial for characterizing the composition and structure of glycoproteins. In this study, Fourier transform infrared spectroscopy (FT-IR) combined with chemometric strategies was used to analyze glycoproteins. The results demonstrate that this approach is efficient in characterizing glycosylation in therapeutic proteins. Support vector regression (SVR) models showed better predictive performances compared to Partial Least Squares Regression (PLSR) models.
Article
Chemistry, Analytical
Joelle De Meutter, Erik Goormaghtigh
Summary: The study used protein microarrays and FTIR imaging to analyze structural modifications in a set of 85 proteins with different secondary structure contents, revealing 6 components in native proteins correlated with alpha-helix, beta-sheet, and other structures, and a new component in denatured proteins potentially representing intermolecular beta-sheet aggregation. MCR-ALS provided insight into potential spectroscopic markers of protein aggregation and other structural rearrangements.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Annaelle Demaude, Kitty Baert, David Petitjean, Juliette Zveny, Erik Goormaghtigh, Tom Hauffman, Michael J. Gordon, Francois Reniers
Summary: In this work, immobilization of unwanted filaments in dielectric barrier discharges (DBD) is achieved for one-step deposition of patterned coatings. By texturing one of the dielectric surfaces, chemical and topographical contrasts at sub-millimeter scale are obtained. The influence of filaments on the deposition process is investigated.
Article
Biochemistry & Molecular Biology
Olivier Suys, Allison Derenne, Erik Goormaghtigh
Summary: Quality control is crucial in the pharmaceutical industry. Researchers have developed a biosensor based on ATR-FTIR spectroscopy to detect immunoglobulins during their production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Jehan Waeytens, Joelle De Meutter, Erik Goormaghtigh, Alexandre Dazzi, Vincent Raussens
Summary: Nanoscale infrared spectroscopy (AFMIR) has become an important tool for analyzing biological samples, particularly protein assemblies, at the nanoscale level. A method for studying the secondary structure of proteins is introduced based on a protein library of 38 well-characterized proteins. Ascending stepwise linear regression (ASLR) and partial least square (PLS) regression were used to correlate characteristic bands in the spectra with major secondary structures (alpha helices and beta sheets). ASLR appears to provide better results than PLS. The secondary structure predictions have a root mean square standard error of 6.39% for alpha helices and 6.23% for beta sheets in cross validation.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Joelle De Meutter, Erik Goormaghtigh
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2020)