Journal
ANALYST
Volume 137, Issue 13, Pages 3160-3165Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an35065c
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Funding
- National Natural Science Foundation of China [21073115, 20604014]
- Natural Science Foundation of Shanxi province [2007021008]
- Program for New Century Excellent Talents in University of China
- Program for the Top Young and Middle-aged Innovative Talents of Higher Learning Institutions of Shanxi province (TYMIT and TYAL)
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This work reports a sensitive amperometric biosensor for organophosphate pesticides (OPs) fabricated by modifying a glassy carbon electrode with acetylcholinesterase (AChE) immobilized on ionic lwiquid-functionalized graphene (IL-G). The functionalized graphene sheets had good dispersibility and longterm stability in various solvents. The as-prepared biosensor showed high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K-m) value of 0.77 mM. Furthermore, based on the inhibition by OPs of the enzymatic activity of the immobilized AChE, and using carbaryl as a model compound, the inhibition of carbaryl was proportional to its concentration ranging from 0.0025 to 0.48 and 0.48 to 1.42 mu g mL(-1) with a detection limit of 0.8 ng mL(-1) (S/N = 3). The developed biosensor exhibited a good performance for OPs detection, including good reproducibility and acceptable stability, which provided a new and promising tool for the analysis of enzyme inhibitors.
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