4.6 Article

Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

Journal

ANALYST
Volume 137, Issue 18, Pages 4119-4122

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an35640f

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Funding

  1. National Institute for Health Research (NIHR) under its Invention for Innovation (i4i) Programme [II-AR-0209-10012]
  2. National Institute for Health Research [II-AR-0209-10012, FSG004] Funding Source: researchfish

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A selective scanning method was used to measure spatially resolved Raman spectra of live Neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. The technique allowed the detection of nucleic acids, lipids and proteins linked to the parasites and their cellular micro-environment at similar to 10x shorter acquisition time compared to raster scanning.

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