Article
Chemistry, Analytical
Yamin Lin, Juqiang Lin, Mengmeng Zheng, Wei Gong, Huan Li, Zhengyu Shu, Weiwei Du, Siqi Gao, Yun Yu
Summary: In this study, a novel extraction-isolation albumin analysis method for prostate cancer screening was developed by utilizing the specific adsorption capacity of hydroxyapatite (HAp) combined with surface-enhanced Raman spectroscopy (SERS). The results showed that the HAp flower particles exhibited superior adsorption-release capacity for albumin and could be used for the diagnosis of prostate cancer through SERS spectral analysis. The PLS-SVM model achieved a high diagnostic accuracy of 95.00% for differentiating normal volunteers from prostate patient groups.
ANALYTICA CHIMICA ACTA
(2022)
Article
Spectroscopy
Xin Bai, Juqiang Lin, Xiang Wu, Yamin Lin, Xin Zhao, Weiwei Du, Jiamin Gao, Zeqin Hu, Qingjiang Xu, Tao Li, Yun Yu
Summary: This study aimed to investigate the diagnostic potential of surface-enhanced Raman spectroscopy (SERS) for bladder cancer and kidney cancer. Three statistical algorithms (PCA-LDA, PLS-RF, and PLS-SVM) were evaluated for their classification ability. The results showed that SERS could effectively differentiate cancer patients from normal subjects based on the analysis of plasma SERS spectra, with high classification accuracy. Among the three algorithms, PLS-SVM demonstrated superior performance and achieved the highest classification accuracy for bladder cancer and kidney cancer.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Mengke Su, Shixuan Yang, Min Xu, Shanshan Du, Liqin Zheng, Xian Wang, Cheng Qu, Honglin Liu
Summary: In this study, a novel SERS probe was developed using nucleic acid-peptide conjugates for high-specific profiling of abnormal protein levels in cancer patients. The probe showed rapid, simple, and multisignal detection capabilities. The effect of low-temperature storage time on blood samples was also analyzed, and it was found that plasma samples stored at 4°C for more than 2 days could generate false negative results due to TB hydrolysis, highlighting the importance of clinical sample analysis. This nucleic acid-peptide conjugate provides new ideas for future SERS sensing strategies.
ANALYTICAL CHEMISTRY
(2023)
Article
Physics, Multidisciplinary
Dominik Wild, Cathrin Theiss, Gerhard Holl
Summary: The identification of energetic materials in containments is a crucial challenge for safety and security. The combined laser drilling method with subsequent Raman spectroscopic measurements through microscopic bore holes offers a solution to quickly and reliably identify target substances, even when shielded by covering materials.
EUROPEAN PHYSICAL JOURNAL PLUS
(2021)
Article
Chemistry, Analytical
Guohua Qi, Xingkang Diao, Shuping Hou, Jiao Kong, Yongdong Jin
Summary: Proteins as the main undertakers of life activities were sensitively detected in the nucleus and mitochondria of cells under electrical stimulation using label-free surface-enhanced Raman spectroscopy. The developed plasmonic SERS sensing platform shows superior sensitivity and repeatability in analyzing protein conformation and expression levels during the stimulation process, providing potential for bioassay and cell studies.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
David Mabwa, Ketankumar Gajjar, David Furniss, Roberta Schiemer, Richard Crane, Christopher Fallaize, Pierre L. Martin-Hirsch, Francis L. Martin, Theordore Kypraios, Angela B. Seddon, Sendy Phang
Summary: This study demonstrates the discrimination of endometrial cancer from benign controls using mid-infrared spectroscopy of dried plasma or serum samples. Different spectral data ranges have an impact on discrimination results, and good classification performance can still be achieved even when a section of the bio-fingerprint region is removed.
Article
Chemistry, Analytical
Chenyu Zhang, Guohua Qi, Jiao Kong, Xingkang Diao, Xingkai Ju, Jiafeng Wang, Shaojun Dong, Yongdong Jin
Summary: In this study, ER-targeting plasmonic nanoprobes were developed to trace molecular stress response profiling within the ER during electrical stimulation. It was found that excessive accumulation of beta-misfolded proteins after electrical stimulation led to disruption of ER homeostasis and mitochondrial dysfunction. Furthermore, increasing voltage levels resulted in calcium ion imbalance and inhibition of tumor cell migration.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Peipei Fang, Hongpeng Wang, Xiong Wan
Summary: This study introduces a method using laser confocal Raman technique combined with theoretical Raman spectra based on DFT to accurately analyze the molecular differences between extra virgin olive oil (EVOO) and low-cost olive oil, and achieve quantitative analysis using chemometrics.
Article
Chemistry, Analytical
Andrea Barucci, Cristiano D'Andrea, Edoardo Farnesi, Martina Banchelli, Chiara Amicucci, Marella de Angelis, Byungil Hwang, Paolo Matteini
Summary: The article proposes an effective machine learning classification method for protein species with similar spectral profiles, based on principal component analysis (PCA) applied to multipeak fitting on SERS spectra. This strategy ensures successful discrimination of proteins and thorough characterization of their chemostructural differences, ultimately paving the way for new applications and diagnostics in the field of life sciences.
Article
Chemistry, Applied
Qianyi Wu, Marciano M. Oliveira, Eva M. Achata, Mohammed Kamruzzaman
Summary: In this study, a benchtop NIR system and a filter-based NIR spectrometer were used with multivariate analysis to rapidly detect multiple allergenic ingredients in gluten-free flour. Only 9 dominant wavelengths were selected and a better predictive model was developed, which outperformed the filter-based NIR method.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Yangcenzi Xie, Yu Wen, Xiaoming Su, Chao Zheng, Ming Li
Summary: A machine learning-driven surface-enhanced Raman spectroscopy (SERS)-integrated strategy is developed for label-free detection of cellular HER2, achieving high accuracy in cell identification and classification for HER2+ breast cancer. The combination of label-free SERS detection and machine learning-driven analysis enables longitudinal monitoring of therapeutic efficacy, aiding in decision-making and management.
ANALYTICAL CHEMISTRY
(2022)
Review
Medicine, General & Internal
Siddra Maryam, Marcelo Saito Nogueira, Rekha Gautam, Shree Krishnamoorthy, Sanathana Konugolu Venkata Sekar, Kiang Wei Kho, Huihui Lu, Richeal Ni Riordain, Linda Feeley, Patrick Sheahan, Ray Burke, Stefan Andersson-Engels
Summary: Oral cancer is the 16th most common cancer worldwide, and early diagnosis is challenging. Optical spectroscopy could be a non-invasive and cost-effective diagnostic technique with advantages such as cost, speed, objectivity, sensitivity, painlessness, and ease-of-use. This review provides a comprehensive overview and recent developments in optical spectroscopy for oral cancer diagnosis, emphasizing the importance of saliva-based potential biomarkers for early-stage diagnosis.
Article
Chemistry, Multidisciplinary
Sravani Kaja, Ashin Varghese Mathews, Venkata Vamsi Krishna Venuganti, Amit Nag
Summary: Surface-enhanced Raman spectroscopy (SERS) is a promising label-free technology for clinical monitoring due to its high sensitivity and multiplexing ability. It can accelerate the screening of important drugs in the blood and plasma of cancer patients in a simpler, faster, and less-expensive manner.
Article
Chemistry, Analytical
Xiaoming Su, Xinyu Liu, Yangcenzi Xie, Mingyang Chen, Hong Zhong, Ming Li
Summary: The widespread abuse of fentanyl has become a major global public health concern, leading to a drastic increase in overdose deaths. A recently developed freestanding surface-enhanced Raman spectroscopy (SERS) biosensor, called the FrEnSERS biosensor, has shown excellent capability in identifying and quantifying trace amounts of fentanyl in biofluids. This biosensor, composed of a reduced graphene oxide membrane decorated with hydrophobic Au nanostars, combines high SERS enhancement and analyte enrichment to achieve remarkable SERS performance. It has demonstrated sensitive and quantitative detection of fentanyl in serum and urine, with a wide dynamic range and low limits of detection. The FrEnSERS biosensor is a promising tool for forensic analysis and clinical diagnosis, providing a sensitive, cost-effective, and reliable method for rapid quantitative analysis of fentanyl in biofluids.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Suyang Li, Junjie Chen, Wanbing Xu, Biao Sun, Jiechen Wu, Qiang Chen, Pei Liang
Summary: In this study, an efficient hybrid microfluidic chip was designed to prepare uniform Au@Ag core-shell nanoparticles, and DTNB was used as the internal standard tag molecule to prepare Au@DTNB@Ag for SERS detection. The experimental results showed that the homogeneous SERS substrate prepared using a microfluidic chip has potential for sensitive and reliable detection of environmental chemical contaminants.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Marine & Freshwater Biology
Gabriela H. Da Silva, Lidiane Silva Franqui, Marcelo A. De Farias, Vera Lucia S. S. De Castro, Hugh J. Byrne, Diego S. T. Martinez, Regina T. R. Monteiro, Alan Casey
Summary: This study investigated the cytotoxicity, protein corona formation, and cellular internalisation of TiO2_MWCNT on RTG-2 cells for the first time. The results showed that this nanohybrid had no toxicity effects on RTG-2 cells within 24 hours. Spectral and microscopic analysis revealed that TiO2_MWCNT can form complexes through adsorption of proteins and be internalised by cells.
AQUATIC TOXICOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Rubina Shaikh, Amuthachelvi Daniel, Fiona M. Lyng
Summary: This review focuses on recent advances in using Raman spectroscopy for cervical cancer detection and discusses the need for new methods due to the limitations of current screening techniques. The potential of Raman spectroscopy combined with statistical analysis has been shown in detecting cervical cancer. The review examines research using exfoliated cells, biofluid samples, and tissue for Raman spectroscopic approaches.
Article
Biochemistry & Molecular Biology
Shane Forde, Giulianna Vozza, David J. Brayden, Hugh J. Byrne, Jesus M. Frias, Sinead M. Ryan
Summary: In order to address the challenges of oral administration of SeMet, researchers encapsulated it in zein-coated nanoparticles made from chitosan using an ionic gelation formulation. The SeMet-NPs showed high stability and minimal cytotoxicity, as well as antioxidant properties. Additionally, they exhibited increased intestinal permeability compared to free SeMet. These findings suggest the potential of SeMet-NPs for further development as an oral supplement.
Article
Multidisciplinary Sciences
Janith Wanigasekara, Lara F. Carroll, Patrick Cullen, Brijesh Tiwari, James Curtin
Summary: Three-dimensional (3D) cell culture models can effectively simulate the natural in vivo environment, shape, tissue stiffness, stressors, gradients, and cellular response, bridging the gap between in vitro cell cultures and in vivo responses, without the associated costs and ethical concerns of animal models.
Article
Chemistry, Multidisciplinary
Furong Tian, Luis Felipe das Chagas e Silva de Carvalho, Alan Casey, Marcelo Saito Nogueira, Hugh J. Byrne
Summary: In recent years, the disease burden of hyperuricemia has been increasing, especially in high-income countries and the economically developing world with a Western lifestyle. Improved methods of uric acid and hypoxanthine detection using surface-enhanced Raman spectroscopy (SERS) and high-performance liquid chromatography (HPLC) were demonstrated by analyzing three different bodily fluids. Gold nanostars were used for quantitative detection of uric acid and hypoxanthine concentrations. The results show strong correlations between HPLC and SERS, and the potential of vibrational spectroscopy in clinical applications was highlighted.
Article
Pharmacology & Pharmacy
Suha Elderderi, Franck Bonnier, Xavier Perse, Hugh J. Byrne, Florent Yvergnaux, Igor Chourpa, Abdalla A. Elbashir, Emilie Munnier
Summary: Raman spectroscopy is a useful technique for quantifying complex cosmetic products without the need for extensive pre-analytical processing. This study demonstrates the effectiveness of Raman spectroscopy coupled with partial least squares regression (PLSR) in quantifying Alginate nanoencapsulated Piperonyl Esters (ANC-PE) in a hydrogel. By dividing the samples into a training set and a test set, the accuracy of the prediction model was evaluated, with a root mean square error of cross-validation (RMSECV) and prediction (RMSEP) of 0.142% and 0.148% (w/w PE), respectively. These results highlight the potential of Raman spectroscopy for rapid and label-free quantification of active cosmetic ingredients in complex formulations, promising future applications in analytical quality control (AQC).
Review
Biochemistry & Molecular Biology
Laura E. Healy, Xianglu Zhu, Milica Pojic, Carl Sullivan, Uma Tiwari, James Curtin, Brijesh K. Tiwari
Summary: Seaweed is gaining attention as a potential sustainable source of nutrition for humans due to its rich nutritional profile and ability to produce functional ingredients. The extraction of compounds such as alginate, agar, and carrageenan from seaweed is well-established, and innovative processing methods are being explored. A wide range of products can be developed using seaweed, including bread, noodles, and yogurt, as well as enhancing the nutritional profile of meat products. This review paper explores the unique properties of seaweed as a food source, the processes involved in seaweed aquaculture, and the challenges facing the industry.
Article
Food Science & Technology
Eduarda M. Cabral, Xianglu Zhu, Marco Garcia-Vaquero, Sara Perez-Vila, Jiafei Tang, Laura G. Gomez-Mascaraque, Mahesha M. Poojary, James Curtin, Brijesh K. Tiwari
Summary: This study aimed to valorize hemp waste from oil extraction as a source of protein by analyzing the effect of high-pressure processing (HPP) pre-treatments combined with conventional or ultrasound-assisted extraction (UAE) methods. The results showed that UAE had advantages over conventional extraction methods in protein recovery, and the HPP pre-treatments had an impact on protein recovery and purity. These findings indicate the potential of using UAE for protein recovery in the food industry and the importance of further research for optimization and cost estimation.
Article
Chemistry, Analytical
Maria Joao Oliveira, Soraia Caetano, Ana Dalot, Filipe Sabino, Tomas R. Calmeiro, Elvira Fortunato, Rodrigo Martins, Eulalia Pereira, Miguel Prudencio, Hugh J. Byrne, Ricardo Franco, Hugo Aguas
Summary: Early and accurate detection of pathogenic microorganisms is crucial for diagnosis and patient outcomes. The combination of a polystyrene-based microfluidic device and Surface-Enhanced Raman Spectroscopy shows excellent sensitivity and specificity in detecting malaria. The system can be easily adapted for other pathogens and has the potential for early diagnosis of infectious diseases.
Article
Chemistry, Analytical
Valentina Notarstefano, Alessia Belloni, Paolo Mariani, Giulia Orilisi, Giovanna Orsini, Elisabetta Giorgini, Hugh J. J. Byrne
Summary: In this study, Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) and Principal Component Analysis (PCA) were used to analyze Raman MicroSpectroscopy (RMS) data of Oral Squamous Carcinoma Cells (OSCC) exposed to the chemotherapeutic agent cisplatin. The results showed that RMS coupled with multivariate analysis can quantitatively monitor the progression of cellular responses to different drugs, providing potential applications for label-free, in vitro, pre-clinical screening.
Review
Endocrinology & Metabolism
Zohreh Mirveis, Orla Howe, Paul Cahill, Nitin Patil, Hugh J. J. Byrne
Summary: In recent years, the analysis of the glutamine metabolic pathway has been a major focus in metabolomics research due to its significance in cell biosynthesis and bioenergetics, especially in cancer cell survival. However, monitoring the kinetics of this pathway in a living cell in real-time and holistically remains a challenge.
Article
Biochemistry & Molecular Biology
Megan O'Shaughnessy, Jasmine Hurley, Shane C. Dillon, Celine Herra, Pauraic McCarron, Malachy McCann, Michael Devereux, Orla Howe
Summary: Antimicrobial resistance (AMR) is a major global health challenge, and there is an urgent need to develop novel therapeutic agents to overcome it. Metal complexes incorporating 1,10-phenanthroline and various dicarboxylate ligands were found to have high antibacterial activity against AMR pathogens. The chelates incorporating both phen and the dianion of 3,6,9-trioxaundecanedioic acid were the most effective, and their activity varied depending on the metal center. Whole-genome sequencing of Pseudomonas aeruginosa exposed to these metal complexes revealed mutations in genes involved in cellular respiration, polyamine biosynthesis, and virulence mechanisms.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jade F. Monaghan, Daniel Cullen, Claire Wynne, Fiona M. Lyng, Aidan D. Meade
Summary: This study evaluated the impact of pre-analytical procedures on the spectral integrity of lymphocytes analyzed using FTIR and Raman spectroscopy. The findings showed that the freeze-thaw cycle, in vitro culture inter-operator variability, and the time to lymphocyte isolation can affect the spectral signature of lymphocytes. However, the lack of standardized protocols for sample preparation, storage, and processing hinders the integration of these techniques into clinical workflows.
Article
Materials Science, Multidisciplinary
Aimee Sheehan, Tatsiana Mikulchyk, Catherine S. P. De Castro, Safakath Karuthedath, Wejdan Althobaiti, Metodej Dvoracek, Hugh J. Sabad-e-Gul, Hugh J. Byrne, Frederic Laquai, Izabela Naydenova, Mikhail A. Filatov
Summary: A series of new heavy-atom-free photosensitizers were designed and applied for holographic recording in a photopolymerizable material. The photoinduced electron transfer and spin-orbit charge transfer intersystem crossing were studied, and highly efficient photosensitization was observed.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
