Article
Chemistry, Applied
Xuetao Hu, Yanxiao Li, Yiwei Xu, Ziyu Gan, Xiaobo Zou, Jiyong Shi, Xiaowei Huang, Zhihua Li, Yahui Li
Summary: Carbon quantum dots synthesized from orange peel were used as a green and efficient fluorescent probe for detecting E. coli, showing high sensitivity and reliability. The fluorescent method based on CQDs-MNPs allows for accurate analysis of contaminated milk samples, demonstrating great potential in ensuring food quality and safety.
Article
Chemistry, Analytical
Nilamben Panchal, Vedant Jain, Rebekah Elliott, Zachary Flint, Paul Worsley, Caine Duran, Tuhina Banerjee, Santimukul Santra
Summary: Increasing foodborne illnesses have created global health and economic burdens, necessitating the development of specific and rapid testing methods for identifying foodborne pathogens. This study introduces a nanoplatform based on nanozymes that can efficiently and accurately identify pathogens in the agrifood industry.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Yanna Shao, Zhengzheng Wang, Jihang Xie, Zhenjun Zhu, Ying Feng, Shubo Yu, Liang Xue, Shi Wu, Qihui Gu, Jumei Zhang, Qingping Wu, Juan Wang, Yu Ding
Summary: We developed a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for detecting Escherichia coli O157:H7. Using polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption, the ICA showed excellent colorimetry signals. In addition, the PDA-AuNPs' absorption spectrum overlapped significantly with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of QDs fluorescence through the inner filter effect. By utilizing the fluorescence intensity changes induced by PDA-AuNPs, the ICA achieved a detection limit of 9.06 x 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor showed reliable and accurate performance in detecting actual samples with a recovery rate between 80.12% and 114.69%.
Article
Chemistry, Analytical
Lishan Zhu, Zhenda Liang, Yongtao Xu, Zhiquan Chen, Jiasi Wang, Li Zhou
Summary: We developed a rapid and highly sensitive method for detecting E. coli O157:H7 using Recombinase-Aided Amplification (RAA) and CRISPR/Cas12a technology. This method showed high sensitivity, detecting as low as 1 CFU/mL (fluorescence method) and 1 x 10^2 CFU/mL (lateral flow assay) of E. coli O157:H7. The detection system could complete the entire process within 55 min and the signal readout could be visualized with a handheld UV lamp or a naked-eye-detected lateral flow assay. This method has a promising application prospect for in situ detection of trace amounts of pathogens due to its speed, sensitivity, and simplicity.
Article
Food Science & Technology
Jin-Hee Kim, Se-Wook Oh
Summary: By utilizing mPCR-LFA in combination with concentration methods, the sensitivity of detecting Escherichia coli O157:H7 and Salmonella Typhimurium was significantly improved. The study results demonstrate that this approach can rapidly and accurately detect these foodborne pathogens within a short period of time.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Polymer Science
Sawsan Mutlaq, Borhan Albiss, Anas A. Al-Nabulsi, Ziad W. Jaradat, Amin N. Olaimat, Mohammad S. Khalifeh, Tareq Osaili, Mutamed M. Ayyash, Richard A. Holley
Summary: A conductometric immunosensor was developed for rapid detection of the foodborne pathogen Escherichia coli O157:H7, showing high sensitivity and fast detection. The sensor demonstrated good detection efficiency in food matrices and has the potential for quick sample processing in food safety and quality control applications.
Article
Food Science & Technology
Mauro D. D. Garcia, Maria J. Ruiz, Luis M. M. Medina, Roberto Vidal, Nora L. L. Padola, Analia I. Etcheverria
Summary: The objective of this study was to characterize six colicinogenic Escherichia coli strains and test their inhibitory activity against STEC O157:H7. The results showed that these strains and their colicins have inhibitory activity against STEC O157:H7. PCR and genome sequencing identified various colicins and microcins in these strains, and no genes coding for stx1 and stx2 were detected. The results suggest that colicins could be a potential alternative for reducing the presence of STEC O157:H7.
Article
Biophysics
Yong Zhao, Yanzhao Li, Pingping Zhang, Ziheng Yan, Yugui Zhou, Yipu Du, Chunyu Qu, Yajun Song, Ding Zhou, Songnan Qu, Ruifu Yang
Summary: The study developed a highly sensitive fluorescent immunosensor using CDs-microspheres for the specific detection of Escherichia coli O157:H7 in milk, showing strong fluorescence intensity, good stability, and high uniformity in fluorescence. The immunosensor had a detection limit of 2.4 x 10(2) CFU/mL and could detect spiked E. coli O157:H7 in milk within 30 minutes with good repeatability. Additionally, the method could detect trace amounts of E. coli O157:H7 in real milk samples with 100% accuracy after bacterial enrichment, demonstrating its potential as a rapid and sensitive tool for pathogen detection in milk and other foods.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
Chuyan Chen, Claudia P. Coronel-Aguilera, Bruce M. Applegate, Andrew G. Gehring, Arun K. Bhunia, George C. Paoli
Summary: The USDA-FSIS has zero tolerance for E. coli O157:H7 in raw ground beef and currently collects samples from beef processing facilities for testing. This study assessed the potential of using a phage-based luminescence detection assay during sample transportation, and the results suggest that this approach is feasible.
Article
Biophysics
Yanna Shao, Wenxing Xu, Yin Zheng, Juan Wang, Jihang Xie, Zhenjun Zhu, Xinran Xiang, Qinghua Ye, Youxiong Zhang, Liang Xue, Bing Gu, Jierong Chen, Jumei Zhang, Qingping Wu, Yu Ding
Summary: This study proposes a novel method of polyallylamine hydrochloride (PAH)-mediated metal growth for the detection of Escherichia coli O157:H7 by using AuNP-ICA. The method achieves highly enhanced colorimetric signals by controlling the growth of Cu shells on the AuNP core using PAH as the growth framework. The introduction of PAH eliminates non-specific adsorption and improves the signal-to-noise ratio for the detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Ziquan Wang, Qian Xu, Siyuan Liu, Yingying Liu, Ying Gao, Meng Wang, Ling Zhang, Haiyan Chang, Qiang Wei, Zhiwei Sui
Summary: A rapid and quantitative method for multiplexed detection of Salmonella, E. coli O157:H7, and S. flexneri in ground beef was developed using a sample pretreatment protocol and flow cytometry, enabling analysis within 1 hour without the need for cultural enrichment. The method demonstrated good recovery rates for the three pathogens and had a detection range of 103 to 108 cells/g, with detection limits of 3.1 x 103 cells/g for Salmonella, 2.1 x 103 cells/g for E. coli O157:H7, and 2.3 x 103 cells/g for S. flexneri in ground beef.
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Qin Xiong, Xiaoyan Feng, Hengyi Xu
Summary: A quick and highly sensitive fluorescent probe-based RAA method was developed and successfully applied in detecting E.coli O157:H7, showing high sensitivity and feasibility for on-site testing in real samples.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Food Science & Technology
Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Jun Sup Lim, Jinsung Choi, Shin Young Park
Summary: The efficacy of dielectric barrier discharge (DBD) plasma treatment for reducing non-pathogenic Escherichia coli and E. coli O157:H7 in oysters was studied. Exposure to DBD plasma for at least 60 minutes resulted in significant reductions in both types of bacteria, with minimal impact on the glycogen content and texture of the oysters. This non-thermal intervention could be a promising method for improving the safety of fresh oysters.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)