Article
Biochemical Research Methods
Christina Wichmann, Petra Roesch, Juergen Popp
Summary: A Raman-compatible method for isolating bacteria from BAL fluid using density gradient centrifugation is proposed in the study. The method is suitable for isolating low concentrations of bacteria from complex matrices, with the isolation gradient itself having no effect on the spectra.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Carmen C. C. Mayorga-Martinez, Marketa Castoralova, Jaroslav Zelenka, Tomas Ruml, Martin Pumera
Summary: In this study, a method based on magnetic microrobots was developed for rapid isolation and identification of Staphylococcus aureus bacteria in milk. The method does not produce harmful compounds for the milk microbiota and can be efficiently applied in food production.
Article
Chemistry, Physical
Renata Chroma, Maria Vilkova, Ivan Shepa, Patrycja Makos-Chelstowska, Vasil Andruch
Summary: Deep eutectic solvents (DES) are considered as potential alternatives to hazardous organic solvents, formed by acceptor and donor of hydrogen bonds. In analytical chemistry, they are used for sample separation before instrumental quantification. Spectroscopy studies of TBAB-Gly-based DESs revealed the existence of hydrogen bonds between TBAB and Gly at all molar ratios.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Oncology
Christoph Krafft, Juergen Popp, Peter Bronsert, Arkadiusz Miernik
Summary: Raman spectroscopy allows for label-free assessment of bladder tissue in intraoperative applications. In a retrospective study, Raman microspectroscopic images were used to identify different components of bladder tissue, including normal bladder, bladder cancer, necrosis, epithelium, and lipid inclusions. Additionally, foreign materials such as microplastic particles and pigments were detected in the specimens. This study demonstrates the potential of Raman spectroscopy for tumor detection and material identification in complex matrices.
Article
Chemistry, Analytical
M. Czaplicka, A. A. Kowalska, A. B. Nowicka, D. Kurzydlowski, Z. Gronkiewicz, A. Machulak, W. Kukwa, A. Kaminska
Summary: This study demonstrates the significant potential of SERS combined with multivariate analysis for distinguishing carcinoma or tumor from normal salivary gland tissues as a rapid, label-free tool in cancer detection. PCA and PLS-DA methods were used to identify differences and classify salivary gland homogenates, showing promise for differentiation between healthy and tumor tissues.
ANALYTICA CHIMICA ACTA
(2021)
Review
Biochemistry & Molecular Biology
Aneta Saletnik, Bogdan Saletnik, Czeslaw Puchalski
Summary: Raman spectroscopy is a vibration, marker-free technique used for analyzing tissue and cell structure and composition at the molecular level, providing accurate material identification and serving as a fingerprint tool for a wide range of compounds. The sensitivity of this method to even minute structural changes allows for comparative tests and facilitates tracking of polymers in plant cell walls, contributing to an increased importance in tissue discovery and definition thanks to advancements in both techniques and technology.
Review
Chemistry, Analytical
A. Silge, Karina Weber, D. Cialla-May, L. Mueller-Boetticher, D. Fischer, J. Popp
Summary: The application of Raman spectroscopic methods in life science has made significant progress, driven by advances in instrumentation, miniaturization, and interdisciplinary dialogue. These advancements have brought new possibilities for optical sensing and chemical imaging, contributing to advanced pharmaceutical development and manufacturing. Raman sensing, with its non-destructive and label-free quality testing capabilities, is particularly valuable for process-related aspects in bioprocessing. Additionally, the trend towards personalized medicine has increased the demand for individually tailored medicines, manufactured in public pharmacies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Environmental Sciences
Deep Raj, Subodh Kumar Maiti
Summary: This review compares the global data on microplastics (MPs) concentration in various environmental matrices and discusses the methods used for their estimation and identification. Previous studies have mainly relied on microscopic observation and Fourier transform infrared (FTIR) analysis, which may introduce errors in the estimation process. Newer techniques such as thermogravimetric analysis-FTIR spectroscopy, Raman microspectroscopy, optical photothermal infrared, and pyrolysis/gas chromatography-mass spectrometry are being used to improve the accuracy of MPs identification and quantification.
LAND DEGRADATION & DEVELOPMENT
(2023)
Review
Biochemistry & Molecular Biology
Barbara Gieroba, Grzegorz Kalisz, Mikolaj Krysa, Maryna Khalavka, Agata Przekora
Summary: This review paper summarizes the application of vibrational spectroscopic techniques, such as infrared and Raman spectroscopies, to track the structure of natural polysaccharides. The molecular structure of polysaccharides, including cellulose, hemicellulose, glucan, starch, chitosan, dextran, and their derivatives, influences their industrial and medical use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Sadin Ozdemir, Ceyhun Akarsu, Omer Acer, Mireille Fouillaud, Laurent Dufosse, Nadir Dizge
Summary: Microplastics are widespread in the environment and pose potential public health challenges. Thermophilic bacteria have been found to be capable of biodegrading microplastics and producing alpha-Amylase.
Article
Spectroscopy
Karlis Berzins, Samuel D. L. Harrison, Claudia Leong, Sara J. Fraser-Miller, Michelle J. Harper, Aly Diana, Rosalind S. Gibson, Lisa A. Houghton, Keith C. Gordon
Summary: Raman and ATR-FTIR spectroscopy were used to analyze 208 breast milk samples as part of a larger research study. Comprehensive qualitative and quantitative analysis was carried out using chemometric methods, revealing the relationship between macronutrients and sample metadata. The study location and primary food sources were found to have a stronger impact on fat composition than its absolute content, highlighting the limitations and challenges of using spectroscopic techniques for this type of analysis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biotechnology & Applied Microbiology
Pegah Sadeghi Vasafi, Bernd Hitzmann
Summary: The detection of anomalies in the milk processing process is crucial for maintaining control over the process, and the Raman spectrometer combined with various classification approaches proved successful in identifying anomalies such as temperature and fat variations, added water, and cleaning solution. Support vector machine and k nearest neighbor algorithms showed promising results with accuracy rates of 81.4% and 84.8% respectively, indicating their capability in appropriately classifying different groups of samples in the milk processing industry.
ENGINEERING IN LIFE SCIENCES
(2022)
Article
Instruments & Instrumentation
Dinesh Dhankhar, Anushka Nagpal, Runze Li, Jie Chen, Thomas C. Cesario, Peter M. Rentzepis
Summary: The study utilized resonance Raman spectra of bacterial carotenoids for bacterial strain identification, and intensity changes due to UV radiation dose to differentiate between live and dead bacteria. Moreover, the resonance-enhanced Raman spectra showed high sensitivity in detecting bacteria in water at lower concentrations than usual. A handheld spectrometer was designed to record the spectra in situ, and the method may also be applied for identifying fungi, viruses, and plants in real time.
APPLIED SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Zhongyue Guo, Yeran Bai, Meng Zhang, Lu Lan, Ji-Xin Cheng
Summary: Antimicrobial resistance poses a major threat to global health and economics, necessitating rapid AST methods. We introduce a rapid AST method based on wide-field mid-infrared photothermal imaging, capable of metabolic imaging for hundreds of bacteria at single-cell resolution within seconds. This method enables probing of perturbed microbial protein synthesis within 1 hour after antibiotic treatment in E. coli cells, providing insights into antibiotic susceptibility.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Shu Wang, Hao Dong, Wanzhu Shen, Yong Yang, Zhigang Li, Yong Liu, Chongwen Wang, Bing Gu, Long Zhang
Summary: In this study, a label-free SERS method was developed for the accurate identification of methicillin-susceptible and methicillin-resistant Staphylococcus aureus using aptamer-guided AgNP enhancement and CNN classification. The method showed 100% identification accuracy for MSSA and MRSA, indicating its potential as a rapid detection tool for drug-sensitive and drug-resistant bacterial strains.