Article
Chemistry, Analytical
Peng Yang, Rongxing Zhou, Jing Hu, Lijie Du, Feng Li, Junbo Chen, Xiandeng Hou
Summary: In this study, a lanthanide encoded logically gated micromachine (LGM-Ln) was constructed using a proximity assay, which can respond to multiple inputs in biological samples. The computational process of this system does not involve strand displacement, and a threshold-free design is employed to generate reliable computation values. By altering the affinity ligands, the LGM-Ln can be extended to sense nucleic acids and proteins in a multi-input mode.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Nicole M. Ralbovsky, Lanfang Zou, Bingming Chen, Nanyan Rena Zhang, Catherine D. G. Hines, Marissa Vavrek, Wendy Zhong, Joseph P. Smith, Xiaodong Bu
Summary: Spatial distribution analysis of metals, metalloids, and non-metals in biological tissues is important in life sciences to understand their roles in biological pathways. Chemical imaging methods can reveal individual spatial distributions of analytes, while LA-ICP-MS is effective for direct elemental analysis with high precision.
Article
Chemistry, Analytical
Premy Shanthamoorthy, Adamo Young, Hannes Rost
Summary: This study quantitatively investigated the conditions necessary for unique metabolite detection in complex backgrounds using three benchmarked mass spectrometry (MS) methods. The results showed that DIA outperformed MS1-only and MRM-based methods in terms of specificity. Additionally, collision energy was identified as an important factor influencing unambiguous compound detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fulin Guan, Xiaochao Xiang, Yuping Xie, Hang Li, Wanjun Zhang, Yang Shu, Jianhua Wang, Weijie Qin
Summary: Extracellular vesicles (EVs) are nanoscale vesicles with a phospholipid bilayer that play important roles in cell-to-cell communication. EVs are promising sources for disease biomarkers and have applications in early-stage diagnostics, disease monitoring, therapeutics, and drug delivery. EVs include two main classes: exosomes and microvesicles, with diverse origins, features, and functions.
ANALYTICAL METHODS
(2021)
Article
Agronomy
Ili Nadhirah Jamil, Salahuddin Sanusi, Mukram Mohamed Mackeen, Normah Mohd Noor, Wan Mohd Aizat
Summary: This study presents the first proteomic report on mangosteen ripening, utilizing SWATH-MS analysis to reveal protein changes during ripening. Postharvest analyses confirmed the physiological changes associated with the proteome changes.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Andrea Raab, Kevin Kubachka, Manuela Strohmaier, Marc Preihs, Joerg Feldmann
Summary: In this study, the previously unknown arsenical compound, dimethylarsyldi-methylarsinic acid, was identified in rice grains. The origin of this compound remains unknown.
ENVIRONMENTAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Robert T. Tessman, Whitney Nolte, Paul Toren, Kim Gibson, Linda Vu, Jennifer Goldman
Summary: A reliable method has been developed for the quantification of TMP and its metabolites in human plasma. The method achieves chromatographic separation in less than 8 minutes and utilizes mass spectrometry for detection.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Jordan L. Tanen, Samuel R. White, Duong Ha, Kaveh Jorabchi
Summary: Elemental analysis of fluorochemicals has gained attention due to increased use of fluorinated compounds. In-plasma ionization has limitations for ICPMS applications, but post-ICP chemical ionization using nanospray has improved F detection sensitivity. This study further investigates post-plasma chemical ionization and demonstrates the impact of excess plasma oxygen and use of reagent ions with different metal centers on F detection. The findings present significant improvements for elemental F analysis, especially for online chromatographic detection.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Martin Kertys, Nela Zidekova, Kristian Prso, Katarina Marakova, Katarina Kmet'ova, Juraj Mokry
Summary: The study developed a sensitive method for quantifying caffeine and its main metabolites in rat plasma. Successfully validated, the method was applied to analyze samples from animal experiments focusing on the metabolism and effects of caffeine.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Agnieszka Priebe, Abdessalem Aribia, Jordi Sastre, Yaroslav E. Romanyuk, Johann Michler
Summary: Massive demand for Li-ion batteries drives the research of new materials for next-generation batteries, such as all-solid-state batteries. TOF-SIMS shows outstanding potential for comprehensive characterization of novel Li-rich nickel manganese cobalt oxide thin films, potential cathode materials. The investigation reveals the presence of overlithiated grains and nanoparticles in the buried part of the thin films.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Jiahao Chen, Ruixia Wang, Minghao Ma, Lirong Gao, Bin Zhao, Ming Xu
Summary: This article reviews the analytical strategies for metal-binding proteins using LA-ICP-MS, including in situ analysis and ex situ analysis. It provides important references for studying the role of metals in life through the introduction of the current status in this field.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Linling Guo, Linrui Li, Zhiyun Xu, Fanchen Meng, Huimin Guo, Peijia Liu, Peifang Liu, Yuan Tian, Fengguo Xu, Zunjian Zhang, Shuai Zhang, Yin Huang
Summary: Metabolic markers play important roles in diagnosing and treating cancers, but effective markers are limited by the lack of metabolite selection approaches. In this study, a network-based strategy was proposed to uncover metabolic markers for NSCLC using mass spectrometry-based metabolomics and KEGG database. By identifying 23 putative metabolic markers related to NSCLC, this study also found potential molecular targets and a panel of eight strong candidate metabolites for NSCLC diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Marketa Simkova, Lucie Kolatorova, Pavel Drasar, Jana Vitku
Summary: A robust and rapid LC-MS/MS method for the quantification of 32 steroid hormones in human plasma was developed. The method was validated for the analysis of C18-, C19-, and C21- steroids, demonstrating its applicability in physiological and pathophysiological conditions. This method will serve as a useful tool for investigating steroid-related diseases and can be expanded to analyze other biological matrices.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemical Research Methods
Andrea L. Larraga-Urdaz, Maria L. Fernandez Sanchez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez
Summary: This paper discusses the current trends in modern medicine towards early diagnosis and prognosis of diseases, as well as the challenges of detecting biomarkers in complex matrices. By exploring different amplification strategies and sensitive elemental mass spectrometry detection techniques, new approaches are proposed to overcome analytical challenges.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Nicholas Birse, Philip McCarron, Brian Quinn, Kimberly Fox, Olivier Chevallier, Yunhe Hong, Ratnasekhar Ch, Christopher Elliott
Summary: Health conscious consumers are increasingly choosing organic foods, but detecting fraud in this area is challenging. Combining DART-MS with a single quadrupole mass spectrometer has shown high accuracy in differentiating organic from conventional vegetables.
Article
Biochemical Research Methods
Andrea L. Larraga-Urdaz, Maria L. Fernandez Sanchez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez
Summary: This paper discusses the current trends in modern medicine towards early diagnosis and prognosis of diseases, as well as the challenges of detecting biomarkers in complex matrices. By exploring different amplification strategies and sensitive elemental mass spectrometry detection techniques, new approaches are proposed to overcome analytical challenges.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Meeting Abstract
Ophthalmology
Ana Alvarez-Barrios, Lydia Alvarez, Montserrat Garcia, Eszter Emri, Imre Lengyel, Rosario Pereiro, Hector Gonzalez-Iglesias
ACTA OPHTHALMOLOGICA
(2022)
Review
Chemistry, Analytical
Marta Aranaz, Eva Valencia-Agudo, Lara Lobo, Rosario Pereiro
Summary: Human biological fluids are diverse and can vary greatly in volume, with special considerations needed for sampling from newborns and infants. Inductively coupled plasma-mass spectrometry (ICP-MS) is a suitable technique for determining trace elements in biological fluids, aiding in biochemical process interpretation and disease diagnosis. Research efforts have been focused on enhancing the capabilities of ICP-MS for analyzing microsamples, with examples of applications in the clinical field.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Candela Melendreras, Sergio Forcada, Maria Luisa Fernandez-Sanchez, Belen Fernandez-Colomer, Jose M. Costa-Fernandez, Alberto Lopez, Francisco Ferrero, Ana Soldado
Summary: Breast milk is an optimal food for newborns, meeting all their nutritional needs. Human milk banks in hospitals provide donated milk for feeding premature infants or infants whose mothers are unable to feed them. The development of Near-Infrared Spectroscopy (NIRS) measures for breast milk analysis showed promising results in quality control of macronutrients.
Article
Chemistry, Analytical
Sergio Forcada, Adrian Sanchez-Visedo, Candela Melendreras, Mario Menendez-Miranda, Jose M. Costa-Fernandez, Luis J. Royo, Ana Soldado
Summary: In this study, a competitive phosphorescent immunosensor was developed for the quantification of AFM1 in milk. The immunosensor utilized Mn:ZnS quantum dots as photoluminescent labels and exhibited a low detection limit and good accuracy.
Article
Chemistry, Analytical
Ana Lores-Padin, Emmanouil Mavrakis, Beatriz Fernandez, Montserrat Garcia, Hector Gonzalez-Iglesias, Rosario Pereiro, Spiros A. Pergantis
Summary: Gold nanoclusters were synthesized and used as labels in immunoprobes for the quantitative determination of cytosolic proteins in individual human retinal pigment epithelium cells. The method was successfully applied to the sequential determination of two proteins through the development of immunoassays with the corresponding gold nanoclusters immunoprobes and the measurement of the signal by single cell - inductively coupled plasma - mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ana Lores-Padin, Beatriz Fernandez, Montserrat Garcia, Hector Gonzalez-Iglesias, Rosario Pereiro
Summary: The lack of matrix-matched reference materials hinders the quantitative analysis of elements and biomolecules in biological samples by LA-ICP-MS. This study proposes a novel matrix-matched calibration strategy by using the same cell line of the sample to create laboratory standards, which mimics the matrix of cultured cells.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Paula Menero-Valdes, Ana Lores-Padin, Beatriz Fernandez, Hector Gonzalez-Iglesias, Rosario Pereiro
Summary: In this study, citrate-stabilized iridium nanoclusters (IrNCs) were synthesized and then chemically modified with lipoic acid (LA). The modified IrNCs were conjugated with specific antibodies to create IrNCs-labelled immunoprobes. These immunoprobes were used in competitive immunoassays to detect immunoglobulin E (IgE) and apolipoprotein E (APOE) using inductively coupled plasma-mass spectrometry (ICP-MS). The results showed that the IrNCs-immunoprobes had high recognition capabilities and signal amplification, with detection limits in the tens of picograms per milliliter of protein.
Article
Chemistry, Analytical
Paula Menero-Valdes, Ana Lores-Padin, Beatriz Fernandez, C. Derrick Quarles Jr, Montserrat Garcia, Hector Gonzalez-Iglesias, Rosario Pereiro
Summary: Single cell-inductively coupled plasma-mass spectrometry (sc-ICP-MS) and laser ablation (LA)-ICP-MS were used to comprehensively study the expression of APOE and claudin-1 in ARPE-19 cells treated with glucose. The introduction of microFAST Single Cell set-up improved cellular transport efficiency, and specific immunoprobes labelled with IrNCs were used for protein determination. The results obtained by sc-ICP-MS were validated with commercial ELISA kits, and the distribution of target proteins in individual cells was revealed by LA-ICP-MS with high resolution images.
Article
Engineering, Biomedical
Alejandro Rodriguez-Penedo, Pablo Rioboo-Legaspi, Andrea Gonzalez-Lopez, Ana Lores-Padin, Rosario Pereiro, Maria del Mar Garcia-Suarez, Maria Dolores Cima-Cabal, Estefania Costa-Rama, Beatriz Fernandez, M. Teresa Fernandez-Abedul
Summary: In this paper, the detection of SARS-CoV-2 is carried out using reverse transcription loop-mediated isothermal amplification, and palladium nanoclusters are used as indicators of the amplification reaction at end point. The catalytic activity of palladium nanoclusters on the oxygen reduction reaction can be electrochemically observed due to the decrease in pH during the reaction. By premodifying flexible and small-size screen-printed electrodes with palladium nanoclusters, and using a simple and small electrochemical equipment, their integration into field-deployable devices is greatly facilitated, enabling faster detection of SARS-CoV-2 as well as future microbial threats after easy adaptation.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Ana Fuentes-Cervantes, Julia Ruiz Allica, Francisco Calderon Celis, Jose M. Costa-Fernandez, Jorge Ruiz Encinar
Summary: The use of nanomaterials in biological systems and their interaction with proteins to form biological corona complexes have great potential in nanobiomedicine but also raise toxicological concerns. Characterizing the protein corona complex is challenging and often requires a combination of techniques. Although inductively coupled plasma mass spectrometry (ICP-MS) is a powerful quantitative technique for nanomaterial characterization, its application in nanoparticle-protein corona studies is limited. However, recent advancements in ICP-MS, particularly in protein quantification through sulfur detection, suggest its potential as a complementary method for characterizing and quantifying the nanoparticle-protein corona complex.
Article
Food Science & Technology
Sergio Forcada, Mario Menendez-Miranda, Carlos Boente, Jose Luis Rodriguez Gallego, Jose M. Costa-Fernandez, Luis J. Royo, Ana Soldado
Summary: The distribution of potentially toxic elements (PTEs) and polycyclic aromatic hydrocarbons (PAHs) in dairy farm production chain was studied in 16 livestock farms in Spain. The results showed that farms near industrial areas had higher levels of PTEs and PAHs in the soil and forage, but not in the milk. However, overall, the levels of contaminants in the soil, forage, and milk were relatively low.
Article
Nutrition & Dietetics
Ana Alvarez-Barrios, Lydia Alvarez, Enol Artime, Montserrat Garcia, Imre Lengyel, Rosario Pereiro, Hector Gonzalez-Iglesias
Summary: This study investigated the imbalance of zinc (Zn) and changes in metalloproteins in early age-related macular degeneration (AMD) using a primary human fetal retinal pigment epithelium (RPE) cell culture model. The study found that Zn concentration decreased in RPE cells, while Cu, Na, and K concentrations increased. Additionally, Zn-regulating proteins and Zn transporters were deregulated, oxidative stress increased, and the expression of antioxidant enzymes changed. This study provides evidence for Zn imbalance during AMD development.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Candela Melendreras, Ana Soldado, Jose M. Costa-Fernandez, Alberto Lopez, Francisco Ferrero
Summary: This work presents a miniaturized, low-cost, and user-friendly near-infrared spectroscopy (NIRS) measurement system for alfalfa quality control. It addresses the significant challenge faced by dairy farm technicians and producers to quickly and accurately assess the forage quality on their farms.
APPLIED SCIENCES-BASEL
(2023)
Proceedings Paper
Materials Science, Biomaterials
Ana Soladado, Jose Manuel Costa, Candela Melendreras, Patricia Lozano Fernandez, Juan Carlos Campo, Marta Valledor, Alberto Lopez, Francisco Ferrero
Summary: This study tested the feasibility of two portable near infrared sensors in detecting adulterated olive oils. The results showed that both sensors achieved a calibration coefficient of determination of at least 0.8 for all adulterations.
2023 IEEE SENSORS APPLICATIONS SYMPOSIUM, SAS
(2023)