Journal
ANALYST
Volume 136, Issue 6, Pages 1157-1166Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00654h
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Funding
- European Commission [037118]
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Simultaneous washing and concentration of functionalized magnetic beads in a complex sample solution were demonstrated by applying a rotational magnetic actuation system to a microfluidic chip under continuous flow conditions. The rotation of periodically arranged small permanent magnets close to the fluidic channel carrying a magnetic bead suspension allows trapping and releasing of the beads along the fluidic channel in a periodical manner. Each trapping and releasing event resembles one washing cycle. A purification efficiency of magnetic beads out of a mixed magnetic and non-magnetic bead sample solution of 83 +/- 4% at a flow rate of 0.5 mu L min(-1), and a magnetic bead recovery or concentration efficiency of 91 +/- 5% were achieved using a flow rate of 0.2 mu L min(-1). The detection performance of the device was experimentally evaluated with two different bioassays, using either streptavidin-coated magnetic beads in combination with biotinylated fluorescent isothiocyanate (FITC), or a mouse antigen (Ag)-antibody (Ab) system.
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