Journal
ANALYST
Volume 133, Issue 11, Pages 1481-1485Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b808735k
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Funding
- NIH [R01 DK080714]
- American Heart Association Greater Southeast Affiliate
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK080714] Funding Source: NIH RePORTER
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The biological events occurring in the body are complex and challenging to decode. The expression, production, secretion and interaction of proteins, peptides and small molecules often occur in a fast manner and at low concentrations. Methods used to quantify these events must be rapid, selective, sensitive and robust. In recent years, new variations of affinity methodologies have been developed to facilitate quantitation of these biomolecules. This review will focus on selected affinity techniques that have described multi-analyte measurement, high sensitivity techniques, or the application of new affinity reagents applied to conventional technologies to measure analytes involved in cell communication and biomarkers produced in specific disease states.
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