Article
Chemistry, Analytical
He Chen, Wanlin Sun, Zhongrong Zhang, Zhexuan Tao, Yuling Qin, Yuan Ding, Limin Wang, Minghua Wang, Xiude Hua
Summary: Two high-sensitivity competitive immune-nanoplatforms based on the inner filter effect (IFE-IN) and magnetic separation (MS-IN) were developed to detect imidacloprid (IMI) using gold nanoparticles (AuNPs). Both platforms showed excellent accuracy and reliability in detecting IMI.
Article
Biochemical Research Methods
Priscila D. Mendonca, Lorenna K. B. Santos, Marcos Foguel, Marco A. B. Rodrigues, Marli T. Cordeiro, Luis M. Goncalves, Ernesto T. A. Marques, Rosa F. Dutra
Summary: The incidence of dengue virus infection has grown dramatically worldwide, affecting 128 countries. The diagnosis is challenging due to similarities with other viruses such as Zika, and the underreporting of cases leads to potentially higher numbers of affected people. An immunosensor using carbon nanotube-ethylenediamine for NS1 detection shows promising results for diagnosis of dengue and Zika viruses.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Nikhil Bhalla, Amir Farokh Payam, Alessio Morelli, Preetam Kumar Sharma, Rhiannon Johnson, Alan Thomson, Pawan Jolly, Francesco Canfarotta
Summary: In this study, a single sensor platform capable of detecting multiple variants of SARS-CoV-2 was developed using synthetic binders and nanoplasmonic-based sensing technology. The sensor can detect Alpha, Beta, and Gamma spike protein variants within 30 minutes, making it a rapid and efficient tool for COVID-19 detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Zixin Jin, Wenjing Jia, Wei Sheng, Meiyi Sun, Lishuai Ren, Dongmei Bai, Shuo Wang, Tingting Ya, Ziwuzhen Wang, Xinshuang Tang
Summary: In this study, a fluorescence immunoassay based on blue-green upconversion nanoparticles and magnetic separation was developed for the simultaneous detection of three typical beta-agonists. The immunoassay showed a wide detection linear range and low detection limits, making it highly valuable for the simultaneous detection of beta-agonists in animal derived food.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Qiaoyu Li, Yanlin Wang, Ling Ling, Liang Qiao, Hui Chen, Chuanfan Ding, Shaoning Yu
Summary: A dual-functional platform using reversible conjunction and off-on signal responses was developed for rapid and specific detection of tumor exosomes. The platform utilized Fe3O4@SiO2@TiO2 particles to capture exosomes and constructed a fluorescence aptasensor for detecting tumor exosomes with improved accuracy. This method showed better performance in distinguishing prostate cancer patients and healthy samples compared to traditional nanoparticle-tracking analysis (NTA) method.
Article
Biology
Andrew T. Sullivan, Vibha Rao, Tyler Rockwood, Jahnavi Gandhi, Sarah Gruzka, Logan O'Connor, Bonnie Wang, Katherine B. Ragan, David Yu Zhang, Dmitriy Khodakov
Summary: Detection of multiple RNA targets can be achieved rapidly and accurately using convective array PCR technology with tunable strand displacement probes, allowing for simultaneous amplification and detection of diverse RNA species with programmable sequence selectivity.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Cunming Hu, Li Ma, Fang Mi, Ming Guan, Chang Guo, Fei Peng, Shijiao Sun, Xiaomei Wang, Tingwei Liu, Jiutong Li
Summary: A highly sensitive and rapid SERS immunoassay for cardiac troponin I (cTnI) was developed using core-shell SERS nanotags, allowing quantification of biomarkers by detecting characteristic Raman peak intensities. The method demonstrated high specificity without cross-reactivity with non-specific proteins, and when combined with magnetic beads, it enabled rapid detection without complex separation procedures. The use of a portable Raman instrument in this study highlights the potential for point-of-care testing and small sample analysis in the early diagnosis of acute myocardial infarction.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dan Song, Jiayao Liu, Wenjuan Xu, Xiangzhi Han, Hongliang Wang, Yuan Cheng, Yuxin Zhuo, Feng Long
Summary: This study has developed a rapid and quantitative detection method for anti-SARS-CoV-2 IgG antibody based on an optofluidic sensor, suitable for sensitive on-site determination. The method exhibits good stability and reliability, with a wider measurement range and easier operation compared to traditional ELISA methods.
Article
Multidisciplinary Sciences
Linh Tran, Sangkwon Park
Summary: A highly sensitive immunosensor using quantum dots and streptavidin was developed for detecting the dengue biomarker NS1, offering a fast, convenient, and sensitive approach. However, interference in human plasma serum solution was observed, suggesting the need for further solutions.
SCIENTIFIC REPORTS
(2021)
Article
Spectroscopy
Hanyu Zheng, Ren Sheng, Huanhuan Li, Waqas Ahmad, Quansheng Chen
Summary: A sensitive luminescent bioassay for the detection of Bacillus cereus (B. cereus) was established using up-conversion fluorescence and magnetic separation technology. The method showed good quantitative ability and high specificity for B. cereus, with good reproducibility and stability.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Applied
Huilin Li, Jingying Yang, Ran Han, Yueyao Wang, Xintong Han, Shuo Wang, Mingfei Pan
Summary: A magnetic-fluorescent immunosensing platform was proposed for rapid and sensitive detection of zearalenone (ZEN) in food. The platform showed a wider linear response range and lower detection limit, and could complete the detection process in a short time, demonstrating great potential for food contaminant detection.
Article
Chemistry, Multidisciplinary
Po-Jung Jimmy Huang, Juewen Liu
Summary: In this study, DNA aptamers for L-lactate were obtained through library-immobilization selection method with high affinity and selectivity. A fluorescent biosensor based on the strand displacement method was constructed, allowing simultaneous detection of lactate and glucose in the same solution. This research expands the application of aptamers to simple metabolites and provides a useful tool for continuous and multiplexed monitoring.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Jiaojiao Wang, Qianfeng Xia, Jie Wu, Yingzi Lin, Huangxian Ju
Summary: The signal amplification strategy based on the CRISPR/Cas13a system was designed for sensitive electrochemical detection of Dengue virus, with a wide linear detection range and low detection limit, showing great potential for early clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Zhengzheng Wang, Xiaoyan Feng, Fangbin Xiao, Xuekun Bai, Qian Xu, Hengyi Xu
Summary: A strategy using magnetic separation combine with fluorescence analysis was proposed for S. aureus detection. By utilizing substances such as APBA and PEG, successful enrichment and detection of S. aureus were achieved.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Karla R. Castro, Grazielle O. Setti, Tassia R. de Oliveira, Monica J. Rodrigues-Jesus, Viviane F. Botosso, Ana P. Perini de Araujo, Edson L. Durigon, Luis C. S. Ferreira, Ronaldo C. Faria
Summary: An electrochemical immunoassay using magnetic beads and enzyme-labeled antibodies was developed for the detection of anti-ZIKV antibodies. The method showed high efficiency in detecting anti-ZIKV antibodies in serum samples, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV.
Article
Virology
Joel Rovnak, Laura A. St Clair, Carley McAlister, Chinemerem P. Ogbu, Jessica Smolenske, Randall J. Cohrs, Rushika Perera
Summary: The virtual 2020 meeting of the Rocky Mountain Virology Association featured talks on recent advancements in virology and prion research, with a keynote presentation on the measles virus paradox. Special invited speakers covered topics such as antiviral effector cell biology, T-cell tropism of Epstein-Barr virus, coronavirus spike proteins, Zika virus vaccination, flavivirus infections, prion disease relation to multiple system atrophy, and discussing virology with non-virologists.
Article
Multidisciplinary Sciences
Stefano Rampini, Peng Li, Dhruv Gandhi, Marina Mutas, Ying Fen Ran, Michael Carr, Gil U. Lee
Summary: Magnetically actuated lab-on-a-chip technologies enable rapid separation of specific biomarkers and cells, while nonlinear magnetophoresis allows precise control of superparamagnetic bead transport on chip surfaces. Optimization of micromagnet shape is crucial for improving the efficiency of magnetically actuated separation techniques.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Justin S. G. Ooi, Shee-Mei Lok
Summary: The flavivirus nonstructural protein 1 (NS1) plays an important role in disease progression, and two new studies reveal the mechanism of neutralization by anti-NS1 antibodies, which have been shown to confer protection against severe dengue disease.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Joseph A. Westrich, Erin E. McNulty, Marisa J. Edmonds, Amy Nalls, Megan R. Miller, Brian D. Foy, Joel Rovnak, Rushika Perera, Candace K. Mathiason
Summary: The pathogenicity and transmission potential of infectious agents are heavily influenced by early events during the asymptomatic or subclinical phase of disease. Subclinical infections, such as those caused by the Zika virus (ZIKV), represent roughly 80% of all human infections and are less understood. Establishing animal models of subclinical infections can help investigate mother-to-offspring transmission mechanisms and other maternal infections that impact foetal development.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
James S. Terry, Loran BR. Anderson, Michael S. Scherman, Carley E. McAlister, Rushika Perera, Tony Schountz, Brian J. Geiss
Summary: New monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid protein, specifically mBG86, have been developed and validated for various analyses. The variable regions of six select clones were sequenced and epitope mapping was performed, showing promising value in the fight against COVID-19.
Review
Virology
Guntur Fibriansah, Xin-Ni Lim, Shee-Mei Lok
Summary: The four serotypes of dengue virus exhibit different morphologies, such as compact spherical, bumpy spherical, and non-spherical clubshape morphologies. The inefficient maturation process leads to the presence of partially immature but infectious viral particles that have different antigenicity profiles. Understanding the molecular determinants and environmental conditions that induce morphological changes in the virus, as well as how potent antibodies interact with these particles, is crucial for designing effective therapeutics or vaccines. Multiple techniques have been used to investigate the structural changes in DENV, revealing key residues and interactions playing important roles in these changes.
Article
Genetics & Heredity
Karla Saavedra-Rodriguez, Corey L. Campbell, Saul Lozano, Patricia Penilla-Navarro, Alma Lopez-Solis, Francisco Solis-Santoyo, Americo D. Rodriguez, Rushika Perera, William C. Black
Summary: Pyrethroids are widely used to control the major vector of dengue, chikungunya, and Zika viruses, Ae. aegypti, but resistance mechanisms evolving in mosquito populations threaten disease control efforts. Two common resistance mechanisms involve knockdown resistance at the voltage-gated sodium channel and enhanced metabolism by detoxification enzymes. The proportions of different responses to pyrethroids are dependent on concentration and genetic background. This study identified genetic variants associated with resistance mechanisms in a pyrethroid-resistant colony from Tapachula, Mexico, providing insights into potential markers of resistance.
Article
Virology
Laura A. St Clair, Ali L. Brehm, Shelby Cagle, Tillie Dunham, Jonathan Faris, Paul Gendler, Monica E. Graham, Sandra L. Quackenbush, Joel Rovnak, Rushika Perera
Summary: Held at Colorado State University's Mountain Campus within the Rocky Mountain National Forest, this year's 21st annual Rocky Mountain National Virology Association meeting brought together 114 scientists and students for a 3-day retreat featuring 31 talks and 30 poster presentations. Discussions centered around advances in research pertaining to viral and prion diseases, with a keynote address focusing on zoonotic coronaviruses and the importance of predicting, preparing, and preventing future viral disease outbreaks.
Article
Virology
Laura A. St Clair, Stephanie A. Mills, Elena Lian, Paul S. Soma, Aritra Nag, Caroline Montgomery, Gabriela Ramirez, Nunya Chotiwan, Rebekah C. Gullberg, Rushika Perera
Summary: During infection with dengue viruses, the lipid landscape within host cells is altered to support viral replication and particle assembly. Fatty acyl-CoAs, which are key intermediates in lipid biosynthesis, play a role in this process. Loss of function of certain enzymes involved in fatty acyl-CoA metabolism leads to increased viral replication and infectious particle release, while loss of others results in decreased protein translation, genome replication, and virus release. The specific location of these enzymes within the cell may contribute to the differences observed in dengue virus infection. Loss of mitochondrial ACOT2 and ACOT7 expression also affects the expression of other ACOTs located in different organelles within the cell, suggesting a complex relationship between ACOTs in the virus lifecycle.
Article
Microbiology
Chasity E. Trammell, Gabriela Ramirez, Irma Sanchez-Vargas, Laura A. St Clair, Oshani C. Ratnayake, Shirley Luckhart, Rushika Perera, Alan G. Goodman
Summary: This study demonstrates that targeting insulin signaling through the repurposing of small molecule drugs can effectively reduce viral replication and transmission of Zika virus. Activation of RNAi and JAK/STAT antiviral pathways by insulin signaling leads to a significant reduction in virus levels in mosquitoes. The findings suggest a potential field delivery approach to reduce virus transmission from mosquitoes to humans.
Article
Parasitology
Nunya Chotiwan, Carlos A. Brito-Sierra, Gabriella Ramirez, Elena Lian, Jeffrey M. Grabowski, Babara Graham, Catherine A. Hill, Rushika Perera
Summary: This study identified several fatty acid synthase genes in Aedes aegypti mosquitoes and found that AaFAS1 is the predominant type in adult mosquitoes. This gene is highly expressed in adult females and facilitates the replication and transmission of dengue viruses.
PARASITES & VECTORS
(2022)
Article
Chemistry, Multidisciplinary
Nathan Feely, Anita Wdowicz, Anne Chevalier, Ying Wang, Peng Li, Fanny Rollo, Gil U. U. Lee
Summary: The development of specific and sensitive immunomagnetic cell separation nanotechnologies is crucial for improving cancer patient outcomes. The use of limited biomarkers for enriching circulating tumor cells (CTCs) has resulted in low CTC yields and purity. This study demonstrates that cell bound MUC16 can be effectively targeted for rapid immunomagnetic extraction of CTCs with high yield, purity, and viability. The results also provide insights into the impact of nanoparticle physical properties on the efficiency of immunomagnetic cell isolation.
Article
Chemistry, Analytical
Kaylee M. Clark, Melissa S. Schenkel, Trey W. Pittman, Isabelle C. Samper, Loran B. R. Anderson, Wisarut Khamcharoen, Suad Elmegerhi, Rushika Perera, Weena Siangproh, Alan J. Kennan, Brian J. Geiss, David S. Dandy, Charles S. Henry
Summary: This study presents a low-cost electrochemical immunoassay method for the fast and accurate detection of SARS-CoV-2 nucleocapsid protein. The method utilizes a simple flow device and modified electrodes, enabling automated immunoassay with good selectivity. It has also been successfully applied to detect multiple SARS-CoV-2 variants.
ACS MEASUREMENT SCIENCE AU
(2022)
Article
Chemistry, Multidisciplinary
Cian Holohan, Nathan Feely, Peng Li, Gerard Curran, Gil U. Lee
Summary: The potential of liquid biopsy samples as a less invasive alternative to tissue biopsies is increasingly recognized. This study optimized the lysis reaction for extracting RNA from human melanoma cells using silica coated superparamagnetic nanoparticles. Surprisingly, the addition of sustainable surfactants did not significantly improve cell lysis or qRT-PCR sensitivity. Higher concentrations of serum albumin in the sample matrix decreased qRT-PCR sensitivity due to increased RNase activity.
Article
Chemistry, Multidisciplinary
Cian Holohan, Sophia Hanrahan, Nathan Feely, Peng Li, John O'Connell, Catherine Moss, Michael Carr, Oya Tagit, Gil U. Lee
Summary: The study found that the choice of viral transport media and freeze-thaw cycles significantly impact the sensitivity of COVID-19 molecular diagnostics assays, affecting the stability of the SARS-CoV-2 viral genome. Testing with glycerol-containing PBS is recommended for high stability and sensitivity, as supported by clinical studies on patient-derived samples.