Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 85, Issue 3, Pages 891-902Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/S0001-37652013000300005
Keywords
rations to chicken; ultrasound-assisted extraction; mixture design; Doehlert design; metals
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Funding
- Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Financiadora de Estudos e Projetos (FINEP)
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In this work, multivariate optimization techniques were used to develop a method based on the ultrasound-assisted extraction for copper, manganese, nickel and zinc determination from rations for chicken nutrition using flame atomic absorption spectrometry. The proportions of extracting components (2.0 mol.L-1 nitric, hydrochloric and acetic acid solutions) were optimized using centroid-simplex mixture design. The optimum proportions of this mixture taken as percentage of each component were respectively 20%, 37% and 43%. Variables of method (sample mass, sonication time and final acid concentration) were optimized using Doehlert design. The optimum values found for these variables were respectively 0.24 g, 18s and 3.6 mol.L-1. The developed method allows copper, manganese, nickel and zinc determination with quantification limits of 2.82; 4.52; 10.7; e 9.69 mu g.g(-1), and precision expressed as relative standard deviation (%RSD, 25 mu g.g(-1), N = 5) of 5.30; 2.13; 0.88; and 0.83%, respectively. This method was applied in the analytes determination from chicken rations collected from specialized commerce in Jequie city (Bahia State/Brazil). Application of paired t-test at the obtained results, in a confidence level of 95%, does not show significant difference between the proposed method and the microwave-assisted digestion.
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