Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 83, Issue 3, Pages 1045-1058Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/S0001-37652011005000017
Keywords
antiproliferative potential; Northeastern Brazilian plants; Myracrodruon urundeuva; sarcoma 180 tumor; seed extracts
Categories
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (FUNCAP)
- Financiadora de Estudos e Projetos (FINEP)
- Banco do Nordeste do Brasil (BNB)
Ask authors/readers for more resources
This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC(50) value of 12.5 (9.5-16.7) mu g/mL] than on glioblastoma SF-295 [IC(50) of 25.1 (17.3-36.3) mu g/mL] and Sarcoma 180 cells [IC(50) of 38.1 (33.5-43.4) mu g/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available