Article
Biotechnology & Applied Microbiology
Margaux Poulalier-Delavelle, Jonathan P. Baker, James Millard, Klaus Winzer, Nigel P. Minton
Summary: Acetogenic bacteria have the ability to convert CO2 into useful chemicals and fuels, making them important for achieving Net Zero. However, introducing cas9-containing vectors into Acetobacterium woodii was not successful due to toxicity and a recognition site for an endogenous restriction-modification system. This study developed a Python script to identify PAM candidates of the A. woodii CRISPR/Cas system and successfully created deletions and insertions using synthetic CRISPR arrays.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jan Herzog, Alexander Mook, Tyll Utesch, Frank R. Bengelsdorf, An-Ping Zeng
Summary: Syngas fermentation processes with acetogens have the potential to reduce CO2 emissions and produce bulk chemicals. This study investigates the thermodynamic limits of acetogens in designing a fermentation process, using an anaerobic reactor and an All-in-One electrode for in-situ H-2 generation. The results show that controlling lactate production and adjusting H-2 formation using automated process control can achieve steady lactate concentrations and improve the production of medium chain fatty acids.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Martijn Diender, James C. Dykstra, Ivette Parera Olm, Serve W. M. Kengen, Alfons J. M. Stams, Diana Z. Sousa
Summary: This study shows that ethanol acts as an important intermediate and co-substrate during carboxydotrophic growth of Clostridium autoethanogenum. The mutant strain lacking the Ald subunit of the main bifunctional aldehyde/alcohol dehydrogenase has poor ethanol oxidation capabilities. The direction of ethanol metabolism is dynamic during carboxydotrophic acetogenesis.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Travis G. Wentz, Benjamin J. M. Tremblay, Marite Bradshaw, Andrew C. Doxey, Shashi K. Sharma, John-Demian Sauer, Sabine Pellett
Summary: This study used bioinformatic analysis to investigate the CRISPR-Cas systems of 241 strains of C. botulinum and C. sporogenes. The results showed that the CRISPR-Cas systems mainly prevent the transfer of botulinum toxin gene clusters, rather than directly targeting the toxin gene clusters themselves. Additionally, comparative genomics analysis revealed that these genomic features often occupy the same hypervariable loci in both species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Colin F. Poole
Summary: Extraction is a common sample preparation technique for chromatographic analysis. It is used when samples are complex, dilute, or contain matrix components that interfere with the separation and detection steps. The solvation parameter model provides a general framework for characterizing biphasic extraction systems and comparing liquid and solid extraction phases. The model is used to explain the features important for selectively enriching target compounds using different extraction techniques. Hierarchical cluster analysis with the solvation parameter model facilitates solvent selection, identification of liquid-liquid distribution systems, and evaluation of different approaches for isolating target compounds from different matrices.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Multidisciplinary Sciences
Celine Foulquier, Antoine Riviere, Mathieu Heulot, Suzanna Dos Reis, Caroline Perdu, Laurence Girbal, Mailys Pinault, Simon Dusseaux, Minyeong Yoo, Philippe Soucaille, Isabelle Meynial-Salles
Summary: The authors identified the previously unknown encoding genes for important enzymes involved in redox balancing in n-butanol production by Clostridium acetobutylicum, and increased n-butanol production through enzyme optimization. This discovery is significant for the development of a homobutanologenic C. acetobutylicum strain.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Chemical
Kinnari M. Shah, Elizabeth Dach, Robert Winton, Hanqing Fan, Ngai Yin Yip
Summary: This study investigates the influence of temperature on the equilibrium partitioning of water, salt, and solvent in temperature swing solvent extraction (TSSE) desalination. The findings reveal a tradeoff between selectivity and productivity and provide a new framework for evaluating TSSE performance. Salt is shown to be a key factor in the partitioning process, affecting the ability of the solvent to extract water at lower temperatures and causing the amines to separate from the aqueous phase. Na+ and Clions are consistently distributed into the solvent phase in equimolar ratios. Furthermore, the study establishes a linear correlation between the natural logarithms of salt activity coefficients and water contents in the organic phase. These results suggest that water-ion interactions are more important than amine-ion interactions in the organic phase, addressing a critical knowledge gap in salt transport understanding.
Article
Biochemistry & Molecular Biology
Pedro P. Madeira, Amber R. Titus, Luisa A. Ferreira, Alexander I. Belgovskiy, Elizabeth K. Mann, Jay Adin Mann, William V. Meyer, Anthony E. Smart, Vladimir N. Uversky, Boris Y. Zaslavsky
Summary: Analysis using attenuated total reflection-Fourier transform infrared spectroscopy revealed that each coexisting phase in aqueous two-phase systems has a unique arrangement of hydrogen bonds. The specific arrangements vary depending on the solutes present in the system, and are correlated with the differences in hydrophobic and electrostatic properties of the phases in the systems studied. This suggests that the arrangement of hydrogen bonds may play a significant role in phase separation.
Article
Engineering, Chemical
Heitor Augusto Duarte, Pedro de Alcantara Pessoa Filho
Summary: This work presents an approach to describe liquid-liquid equilibrium in metal ion extraction processes with high metal ion loadings in industrial conditions. The study successfully predicts and describes the extraction of cobalt from multicomponent solutions at total metal ion loadings of approximately 45%, typical of industrial production and separation operations.
MINERALS ENGINEERING
(2021)
Article
Materials Science, Ceramics
Omer Guler, Muekremin Tekeli, Mustafa Taskin, Seval Hale Guler, I. S. Yahia
Summary: The study optimized production parameters of liquid phase exfoliation method for graphene production, determining NMP as the most efficient liquid media and finding that a new hybrid solvent with SDS showed higher efficiency than NMP. The research also observed that 3-5 layer graphene content ranged between 15 and 19 wt% in the produced graphene samples, with the most efficient production seen in hybrid solvents containing NMP or SDS.
CERAMICS INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Valfredo Azevedo Lemos, Luana Bastos Santos, Rosivan Santos Assis
Summary: A method based on ultrasound-assisted liquid-phase microextraction was developed for the determination of vanadium in food and environmental water samples. This method follows the principles of green chemistry and uses a deep eutectic solvent for preconcentration.
MICROCHEMICAL JOURNAL
(2022)
Review
Chemistry, Analytical
Idaira Pacheco-Fernandez, Raul Gonzalez-Martin, Francisca A. e Silva, Mara G. Freire, Veronica Pino
Summary: The successful mode of liquid-phase microextraction (LPME) is dispersive liquid-liquid microextraction (DLLME), with advances focused on utilizing green, smart, and tunable materials as extraction solvents to enhance sustainability and efficiency. Hydrophilic media are considered a promising alternative for improving mass transfer and extraction efficiency.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Chemical
Ying-Chieh Hung, Chieh-Ming Hsieh, Hiroshi Machida, Shiang-Tai Lin, Yusuke Shimoyama
Summary: This study investigates the phase behavior of phase separation solvents before and after CO2 absorption using a predictive model. The results reveal that the hydrophilicity of amines affects the molecular interactions and miscibility of the solvents. The study suggests that amines with balanced interactions with water and glycol ether are potential candidates for CO2 capture phase separation solvents.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Chemistry, Analytical
Vasil Andruch, Alina Kalyniukova, Justyna Plotka-Wasylka, Natalia Jatkowska, Denys Snigur, Serhii Zaruba, Julia Platkiewicz, Agnieszka Zgola-Grzeskowiak, Justyna Werner
Summary: Sustainable development has become a necessity in all areas of human activity, and green chemistry is closely related to it. In the field of analytical chemistry, various approaches have been proposed to meet the requirements of green chemistry, such as the research of new reagents and solvents for analytical purposes. Deep eutectic solvents (DESs) have been investigated and used in analytical chemistry since the mid-2000s, leading to a significant increase in published works in this area. This paper focuses specifically on the applications of DESs in liquid-liquid (micro)extraction (LLME) procedures for the determination of organic and inorganic analytes in different matrices. The use of DESs in sorbent-based procedures will be discussed in a separate paper.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Environmental
Huijun Cui, Xiaoshan Zhu, Yanjie Zhu, Yuxiong Huang, Baiyang Chen
Summary: The study found that intensified disinfection of wastewater during the COVID-19 pandemic may lead to toxicity of toxic disinfection byproducts on microalgae, altering the structure of phytoplankton communities and promoting the competitive advantage of cyanobacteria in co cultures.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Physical
Derren J. Heyes, Samantha J. O. Hardman, David Mansell, Aisling Ni Cheallaigh, John M. Gardiner, Linus O. Johannissen, Gregory M. Greetham, Michael Towrie, Nigel S. Scrutton
JOURNAL OF PHYSICAL CHEMISTRY B
(2017)
Article
Chemistry, Organic
J. Waller, H. S. Toogood, V. Karuppiah, N. J. W. Rattray, D. J. Mansell, D. Leys, J. M. Gardiner, A. Fryszkowska, S. T. Ahmed, R. Bandichhor, G. P. Reddy, N. S. Scrutton
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Syed Mobasher Ali Abid, Sana Aslam, Sumera Zaib, Syeda Mahwish Bakht, Matloob Ahmad, Muhammad Makshoof Athar, John M. Gardiner, Jamshed Iqbal
Article
Chemistry, Physical
Vijaykumar Karuppiah, Kara E. Ranaghan, Nicole G. H. Leferink, Linus O. Johannissen, Muralidharan Shanmugam, Aisling Ni Cheallaigh, Nathan J. Bennett, Lewis J. Kearsey, Eriko Takano, John M. Gardiner, Marc W. van der Kamp, Sam Hay, Adrian J. Mulholland, David Leys, Nigel S. Scrutton
Article
Plant Sciences
Aisling Ni Cheallaigh, David J. Mansell, Helen S. Toogood, Shirley Tait, Antonios Lygidakis, Nigel S. Scrutton, John M. Gardiner
JOURNAL OF NATURAL PRODUCTS
(2018)
Article
Chemistry, Physical
Andrew Currin, Mark S. Dunstan, Linus O. Johannissen, Katherine A. Hollywood, Maria Vinaixa, Adrian J. Jervis, Neil Swainston, Nicholas J. W. Rattray, John M. Gardiner, Douglas B. Kell, Eriko Takano, Helen S. Toogood, Nigel S. Scrutton
Article
Chemistry, Medicinal
Binto Simon, Claire Walmsley, Victoria J. Jackson, Edward P. Garvey, Martin J. Slater, David J. Berrisford, John M. Gardiner
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2019)
Article
Chemistry, Multidisciplinary
Issa S. Issa, Helen S. Toogood, Linus O. Johannissen, James Raftery, Nigel S. Scrutton, John M. Gardiner
CHEMISTRY-A EUROPEAN JOURNAL
(2019)
Article
Chemistry, Multidisciplinary
Ahmed M. Mkadmh, Rami Y. Morjan, James Raftery, Adel M. Awadallah, John M. Gardiner
ARABIAN JOURNAL OF CHEMISTRY
(2020)
Article
Chemistry, Organic
Robin A. Jeanneret, Charlotte E. Dalton, John M. Gardiner
JOURNAL OF ORGANIC CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Saravanan Kandasamy, George F. S. Whitehead, Inigo J. Vitorica-Yrezabal, John M. Gardiner
Summary: 5-O-protected and 1,2-acetonide-protected D-glucurono-6,3-lactone furanosides were converted into novel furano-glucuronamides through treatment with ammonia. Different protection and deprotection routes led to primary gluconamide derivatives, while benzylations led to silyl migration, N-benzylation, or reclosure to lactone. The use of 5-O-PMB protection provided a method for obtaining fully protection-differentiated glucofuranamide. Crystal structures of fully protected glucurono-6,3-lactone lactone and glucuronamide derivatives were reported.
CARBOHYDRATE RESEARCH
(2023)
Article
Chemistry, Organic
Fahad Ayesh Alharthi, Garrett T. Potter, Gordon C. Jayson, George F. S. Whitehead, Inigo J. Vitorica-Yrezabal, John M. Gardiner
Summary: Reactions of a glucuronic acid (GlcA) beta-thioglyco-side with cyclohexadione result in the formation of two expected all-trans decalin-type O2,O3 and O3,O4 cyclohexane-1,2-diacetals (CDAs), along with an epimer of the main O2,O3 acetal. This trans-cis isomer is then converted to higher amounts of the two all-trans products. Isomerization studies demonstrate slow interconversion between the all-trans CDA acetals, with only one significant interconversion with the minor 2,3-diastereomer. Crystal structures of all three isomers are included. These findings are important for understanding the occurrence and interconversion of apparently disfavored isomers in other uses of CDA protections.
Article
Crystallography
Rami Y. Morjan, Said M. El-Kurdi, Jannat N. Azarah, Neda A. Eleiwa, Omar S. Abu-Teim, Adel M. Awadallah, James Raftery, John M. Gardiner
ACTA CRYSTALLOGRAPHICA SECTION E-CRYSTALLOGRAPHIC COMMUNICATIONS
(2018)
Meeting Abstract
Chemistry, Multidisciplinary
Robin Jeanneret, Charlotte Dalton, Gordon Jayson, John Gardiner
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Biochemistry & Molecular Biology
A. Christy Hunter, Yewande Inatimi Oni, Howard T. Dodd, James Raftery, John M. Gardiner, Megan Uttley
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2017)
