Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 377, Issue -, Pages 617-624Publisher
ELSEVIER
DOI: 10.1016/j.ijms.2014.06.023
Keywords
ETD; IIPT; Protein derivatization
Funding
- National Institutes of Health [GM37537, AI33993]
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Previously, we described implementation of a front-end ETD (electron transfer dissociation) source for an Orbitrap instrument [1]. This source facilitates multiple fills of the C-trap with product ions from ETD of intact proteins prior to mass analysis. The result is a dramatic enhancement of the observed ion current without the need for time consuming averaging of data from multiple mass measurements. Here we show that ion-ion proton transfer (IIPT) reactions can be used to simplify ETD spectra and to disperse fragment ions over the entire mass range in a controlled manner. We also show that protein derivatization can be employed to selectively enhance the sequence information observed at the N- and C-termini of a protein. (C) 2014 Elsevier B.V. All rights reserved.
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