Journal
AMINO ACIDS
Volume 40, Issue 2, Pages 689-696Publisher
SPRINGER
DOI: 10.1007/s00726-010-0703-3
Keywords
Oncorhynchus mykiss; Plasma amino acid kinetics; Amino acid turnover; Bolus injection; N-15; Fish
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC
Ask authors/readers for more resources
To gain insight into the metabolic design of the amino acid carrier systems in fish, we injected a bolus of N-15 amino acids into the dorsal aorta in mature rainbow trout (Oncorhynchus mykiss). The plasma kinetic parameters including concentration, pool size, rate of disappearance (R (d)), half-life and turnover rate were determined for 15 amino acids. When corrected for metabolic rate, the R (d) values obtained for trout for most amino acids were largely comparable to human values, with the exception of glutamine (which was lower) and threonine (which was higher). R (d) values ranged from 0.9 mu mol 100 g(-1) h(-1) (lysine) to 22.1 mu mol 100 g(-1) h(-1) (threonine) with most values falling between 2 and 6 mu mol 100 g(-1) h(-1). There was a significant correlation between R (d) and the molar proportion of amino acids in rainbow trout whole body protein hydrolysate. Other kinetic parameters did not correlate significantly with whole body amino acid composition. This indicates that an important design feature of the plasma-free amino acids system involves proportional delivery of amino acids to tissues for protein synthesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available