Article
Biochemistry & Molecular Biology
Jaka Kragelj, Thibault Orand, Elise Delaforge, Laura Tengo, Martin Blackledge, Andres Palencia, Malene Ringkjobing Jensen
Summary: Intrinsically disordered proteins (IDPs) can form promiscuous interactions with their protein targets, facilitated by folding-upon-binding into different conformations. Specific surface properties of the kinases p38 alpha and JNK1 dictate the bound conformation of the regulatory domain MKK4, with enthalpy-entropy compensation playing a major role in maintaining comparable binding affinities towards the two kinases.
Article
Multidisciplinary Sciences
Helina Heino, Lassi Rieppo, Tuija Mannisto, Mikko J. Sillanpaa, Vesa Mantynen, Simo Saarakkala
Summary: ATR-FTIR spectroscopy coupled with machine learning-based PLS-DA was used to study the detection of SARS-CoV-2 from nasopharyngeal swab samples. The results indicate that the ATR-FTIR spectrum contains specific information for SARS-CoV-2 infection, but the diagnostic performance is moderate.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Mohd Younus Bhat, Irfan Mir, Laishram Rajendrakumar Singh, Mahboobul Hussain, Tanveer Ali Dar
Summary: Intrinsically disordered proteins (IDPs) play important roles in cellular processes and disease processes, and trehalose affects the structural-functional elasticity of IDPs.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Immunology
Simona Rolla, Stefania Federica De Mercanti, Valentina Bardina, Alessandro Maglione, Daniela Taverna, Francesco Novelli, Eleonora Cocco, Anton Vladic, Mario Habek, Ivan Adamec, Pietro Osvaldo Luigi Annovazzi, Dana Horakova, Marinella Clerico
Summary: Alemtuzumab has long-term efficacy in the treatment of patients with relapsing multiple sclerosis by increasing the percentage of Treg cells, restoring their suppressor function, and reducing the number of self-reactive cells through immune regulation. These findings contribute to a better understanding of the therapeutic mechanism of alemtuzumab.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Khaled N. M. Khalili, Peter Peinder, Jacqueline Donkers, Richard J. A. Gosselink, Pieter C. A. Bruijnincx, Bert M. Weckhuysen
Summary: Technical lignins present challenges in controlling their structural properties, but the combination of operando ATR-IR spectroscopy and chemometrics allows real-time monitoring of lignin depolymerization, with potential application in other lignin depolymerization processes.
Article
Biochemistry & Molecular Biology
Patricia Santofimia-Castano, Bruno Rizzuti, Angel L. Pey, Maria Esther Farez-Vidal, Juan L. Iovanna, Jose L. Neira
Summary: Plakophilin 1 (PKP1) and NUPR1 were found to interact in the nucleus of cells, potentially playing a role in carcinogenesis through modulation of PKP1 function. The binding affinity between PKP1 and NUPR1 was determined to be in the low micromolar range, involving specific typtophan residues of PKP1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Jaka Kragelj, Rania Dumarieh, Yiling Xiao, Kendra K. K. Frederick
Summary: Frozen proteins at cryogenic temperatures can provide detailed conformational restraints for DNP MAS NMR studies. Using peak shapes, conformational features of individual conformers in the ensemble can be assigned through simulation. This has important implications for studying proteins with intrinsically disordered regions in living cells.
Article
Biochemistry & Molecular Biology
Evgeniya V. Smirnova, Tatiana V. Rakitina, Rustam H. Ziganshin, Georgij P. Arapidi, George A. Saratov, Anna A. Kudriaeva, Alexey A. Belogurov
Summary: The study revealed the interaction network of MBP in mammalian cells, showing its connections with proteins involved in the protein translation machinery and RNA biogenesis.
Article
Spectroscopy
Ali Kocak
Summary: It has been shown that hair can accumulate and trap drugs, providing an indication of long-term exposure. Studying changes in the structure of hair and the effects of cosmetic treatments can help understand false positive results in drug testing and the impact of drugs on hair structure.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Biochemistry & Molecular Biology
Owen Michael Morris, James Hilary Torpey, Rivka Leah Isaacson
Summary: This review evaluates the anatomy of intrinsically disordered proteins (IDPs) and how their intrinsic properties allow for diversity and different modes of interaction. It also provides a detailed overview of the types of disordered domains and the kinetic and thermodynamic principles governing their formation, with reference to a recent example.
Article
Biochemistry & Molecular Biology
Salome Araujo-Abad, Maria Fuentes-Baile, Bruno Rizzuti, J. Fernando Bazan, Adrian Villamarin-Ortiz, Miguel Saceda, Eduardo Fernandez, Miguel Vidal, Olga Abian, Adrian Velazquez-Campoye, Camino de Juan Romero, Jose L. Neira
Summary: RYBP is a multifunctional IDP involved in transcriptional regulation, while PADI4 is a well-folded protein implicated in arginine conversion. Both proteins were found to associate in the nucleus and cytosol of cancer cells, suggesting a potential role in cancer development and progression.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemical Research Methods
Jhullian J. Alston, Andrea Soranno, Alex S. Holehouse
Summary: Over the past two decades, intrinsically disordered proteins and protein regions have been recognized as essential drivers of cellular function, with singlemolecule fluorescence spectroscopy and molecular simulations providing insight into their behavior. When combined, these techniques offer complementary information that can help uncover complex molecular details.
Article
Biochemistry & Molecular Biology
Rifat Farhana, Ruipeng Lei, Khoa Pham, Valerie Derrien, Jonathan Cedeno, Veronica Rodriquez, Sophie Bernad, Francisco Fernandez Lima, Jaroslava Miksovska
Summary: Several novel members of the vertebrate globin family with unique structural features have been discovered. Structural tools were used to investigate the stability of hexacoordinate globin X (GbX) from zebrafish. The results suggest that additional structural determinants contribute to the stability of GbX.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
L. Ramya, S. Helina Hilda
Summary: Multiple Sclerosis (MS) is a demyelinating disease that disrupts the flow of brain signals. This article focuses on the role of intrinsically disordered proteins (IDP) in MS, and finds that the IDRs of these proteins play a significant role in maintaining myelin integrity.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2023)
Article
Multidisciplinary Sciences
David Buhrke, Norbert Michael, Peter Hamm
Summary: Phytochromes are commonly found photoreceptor proteins that undergo secondary structure refolding in response to chromophoric group photoisomerization. Two-dimensional infrared absorption spectroscopy is used to study the photoreaction of bacterial phytochrome Agp1, showing that dipole coupling between the chromophore and the tongue region plays a role in stabilizing the protein in the light-activated state.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Endocrinology & Metabolism
Vladimir V. Bamm, Arielle M. Geist, George Harauz
METABOLIC BRAIN DISEASE
(2017)
Article
Biochemistry & Molecular Biology
Vladimir V. Bamm, Mary E. L. Henein, Shannon L. J. Sproul, Danielle K. Lanthier, George Harauz
FREE RADICAL BIOLOGY AND MEDICINE
(2017)
Article
Biochemistry & Molecular Biology
Kyrylo Bessonov, Kenrick A. Vassall, George Harauz
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2017)
Editorial Material
Biochemical Research Methods
George Harauz
Article
Biochemistry & Molecular Biology
Morgan G. Stykel, Kayla Humphries, Mathew P. Kirby, Chris Czaniecki, Tinya Wang, Tammy Ryan, Vladimir Bamm, Scott D. Ryan
Article
Chemistry, Multidisciplinary
Katharina Widder, Jennica Traeger, Andreas Kerth, George Harauz, Dariush Hinderberger
Article
Multidisciplinary Sciences
Tammy Ryan, Vladimir V. Bamm, Morgan G. Stykel, Carla L. Coackley, Kayla M. Humphries, Rhiannon Jamieson-Williams, Rajesh Ambasudhan, Dick D. Mosser, Stuart A. Lipton, George Harauz, Scott D. Ryan
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Katharina Widder, George Harauz, Dariush Hinderberger
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Article
Cell Biology
Jennica Traeger, Katharina Widder, Andreas Kerth, George Harauz, Dariush Hinderberger
Review
Microbiology
Vladimir V. Bamm, Jordan T. Ko, Iain L. Mainprize, Victoria P. Sanderson, Melanie K. B. Wills
Article
Biophysics
Justin Medeiros, Vladimir V. Bamm, Catherine Jany, Carla Coackley, Meaghan E. Ward, George Harauz, Scott D. Ryan, Vladimir Ladizhansky
Summary: Alpha-synuclein is a small presynaptic protein believed to be crucial in Parkinson's disease pathogenesis. Recent evidence suggests its interaction with mitochondrial membranes and a unique anionic phospholipid. Through NMR experiments, it was found that alpha-syn associated with specific lipid vesicles exhibited more favorable dynamic properties for conducting three-dimensional experiments.
BIOMOLECULAR NMR ASSIGNMENTS
(2021)
Article
Cell Biology
Morgan G. Stykel, Kayla M. Humphries, Evelyn Kamski-Hennekam, Brodie Buchner-Duby, Natalie Porte-Trachsel, Tammy Ryan, Carla L. Coackley, Vladimir V. Bamm, George Harauz, Scott D. Ryan
Summary: Neuronal loss in Parkinson's disease is linked to impaired proteostasis and accumulation of alpha-syn microaggregates in dopaminergic neurons, which promote the spread of pathology between synaptically linked neurons. Using human pluripotent stem cell models, researchers found that SNCA mutant neurons accumulate alpha-syn deposits that can lead to the seeding of alpha-syn pathology. This suggests a new mechanism for the spread of synucleinopathy in PD.
Article
Cell & Tissue Engineering
Nishanth Lakshman, Clara Bourget, Ricky Siu, Vladimir V. Bamm, Wenjun Xu, George Harauz, Cindi M. Morshead
Summary: The study demonstrates that myelin basic protein (MBP) can regulate neural precursor cell (NPC) behavior and that the inhibitory effects of MBP on NPCs are regionally distinct. The heat-labile protein released by spinal cord niche cells plays a key role in mediating the inhibitory actions of MBP on NPCs. These findings highlight the importance of MBP and regionally distinct microenvironments in regulating NPC behavior, with implications for stem cell-based regenerative strategies.