Journal
AMINO ACIDS
Volume 39, Issue 5, Pages 1521-1536Publisher
SPRINGER
DOI: 10.1007/s00726-010-0621-4
Keywords
Phospholipase A(2) activity; Lysophospholipids; Cyclodextrin; TauT; VSOAC; Cell volume regulation
Categories
Funding
- Danish Council for Independent Research/Natural Sciences [272-07-0530, 272-08-0170, 21-04-0535]
- Danish Council for Independent Research/Medical Sciences [271-08-0520]
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In mammalian cells, the organic osmolyte taurine is accumulated by the Na-dependent taurine transporter TauT and released though the volume- and DIDS-sensitive organic anion channel. Incubating Ehrlich Lettr, tumor cells with methyl-beta-cyclodextrin (5 mM, 1 h) reduces the total cholesterol pool to 60 +/- A 5% of the control value. Electron spin resonance data indicate a concomitant disruption of cholesterol-rich micro-domains. Active taurine uptake, cellular taurine content, and cell volume are reduced by 50, 20 and 20% compared to control values, respectively, whereas the passive taurine release is increased 4.5-fold under isotonic conditions following cholesterol depletion. However, taurine release under isotonic conditions is insensitive to DIDS and inhibitors of the volume-regulated anion channel. Uptake and release of meAIB are similarly affected following cholesterol depletion. Kinetic analysis reveals that cholesterol depletion increases TauT's affinity toward taurine but reduces its maximal transport capacity. Cholesterol depletion has no impact on TauT regulation by protein kinases A and C. Phospholipase A(2) activity, which is required for the activation of volume-sensitive organic anion channel (VSOAC), is increased under isotonic and hypotonic conditions following cholesterol depletion, whereas taurine release under hypotonic conditions is reduced following cholesterol depletion. Hence, acute cholesterol depletion of Ehrlich Lettr, cells leads to reduced TauT and VSOAC activities and at the same time increases the release of organic osmolytes via a leak pathway different from the volume-sensitive pathways for amino acids and anions.
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