Article
Immunology
Carina M. Hall, Nathan E. Stone, Madison Martz, Shelby M. Hutton, Ella Santana-Propper, Lora Versluis, Kieston Guidry, Marielisa Ortiz, Joseph D. Busch, Trevor Maness, Jonathan Stewart, Tom Sidwa, Jay E. Gee, Mindy G. Elrod, Julia K. Petras, Maureen C. Ty, Christopher Gulvik, Zachary P. Weiner, Johanna S. Salzer, Alex R. Hoffmaster, Sarai Rivera-Garcia, Paul Keim, Amanda Kieffer, Jason W. Sahl, Fred Soltero, David M. Wagner
Summary: Burkholderia thailandensis, a bacterium closely related to the pathogen B. pseudomallei, was found in water in Texas and Puerto Rico, as well as soil in Mississippi in the United States. This discovery highlights a potential public health risk, although human infections with B. thailandensis are rare.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
George Oduro, F. J. Lourens Robberts, Paul K. A. Dartey, Alex Owusu-Ofori, Chris Oppong, Thomas K. Gyampomah, Isaac Boakye, Emmanuel Quainoo, David P. AuCoin, Danielle Clark, Kevin L. Schully
Summary: This study presents the first confirmation of the presence of B. pseudomallei in the environment of Ghana. It highlights a disease with the potential to cause significant morbidity and mortality in Ghana, which has gone completely unrecognized until now. Furthermore, it encourages local clinicians to familiarize themselves with the diagnosis and clinical management of melioidosis, and laboratories to develop capacity for the safe isolation and identification of B. pseudomallei.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Carina M. M. Hall, Nawarat Somprasong, Johannah P. P. Hagen, Roxanne Nottingham, Jason W. W. Sahl, Jessica R. R. Webb, Mark Mayo, Bart J. J. Currie, Yuwana Podin, David M. M. Wagner, Paul Keim, Herbert P. P. Schweizer
Summary: Cefiderocol is a siderophore cephalosporin designed for treating infections caused by beta-lactam and multidrug-resistant Gram-negative bacteria. Resistance in clinical B. pseudomallei isolates from Australia is caused by an uncharacterized mechanism. The PiuA outer membrane receptor plays a major role in cefiderocol nonsusceptibility in isolates from Malaysia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Karoline Assig, Sabine Lichtenegger, Linh N. H. Bui, Bettina Mosbacher, Anh T. N. Vu, Daniel Erhart, Trung T. T. Trinh, Ivo Steinmetz
Summary: Although B. pseudomallei PCR-based detection is important for environmental monitoring, its culture remains a challenge. Recently, it has been found that using a new ACER medium based on acidic conditions can significantly improve the isolation and cultivation of B. pseudomallei from soil. This new medium helps reduce the false-negative rate in environmental cultures from high-risk areas.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Tushar Shaw, Karoline Assig, Chaitanya Tellapragada, Gabriel E. Wagner, Madhu Choudhary, Andre Goehler, Vandana Kalwaje Eshwara, Ivo Steinmetz, Chiranjay Mukhopadhyay
Summary: Melioidosis is a seasonal infectious disease caused by the soil bacterium Burkholderia pseudomallei. This study conducted in South West India found a wide distribution of B. pseudomallei in soil, with significant variations in abundance between sites. The results indicate that nutrient-depleted habitats promote the presence of B. pseudomallei, and the abundance of B. pseudomallei in soil is highest during the rainy season, when melioidosis cases occur.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Immunology
Vanitha Mariappan, Kumutha Malar Vellasamy, Muttiah Barathan, A. S. Smiline Girija, Esaki M. Shankar, Jamuna Vadivelu
Summary: B. pseudomallei infection causes melioidosis with varying severity, diverse pathological manifestations, complex mechanisms of latency and relapse, and the crucial role of immune response in eradicating the pathogen. However, the question of why some individuals are asymptomatic while others are life-threatening remains unanswered.
FRONTIERS IN IMMUNOLOGY
(2021)
Letter
Immunology
Himali S. Jayasinghearachchi, Vaithehi R. Francis, Harindra D. Sathkumara, Shivankari Krishnananthasivam, Jayanthi Masakorala, Thilini Muthugama, Aruna D. De Silva, Enoka M. Corea
Summary: A cluster of melioidosis cases occurred in eastern Sri Lanka, with four infections caused by Burkholderia pseudomallei isolates of sequence type 594. Whole-genome analysis revealed genetic diversity among the isolates and showed that the cluster was nonclonal.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Shengyuan Huo, Xiao Li, Shiwei Wang, Pan Wu, Dongqi Nan, Chenglong Rao, Qian Li, Xuhu Mao, Jingmin Yan
Summary: This study investigated the structural elucidation of type B O antigen from a clinical B. pseudomallei strain and the effects of different types of LPS on macrophage differentiation. The O antigen was found to consist of repeating units with specific substitutions. Both type A and type B O antigens could polarize macrophages towards the M1 phenotype. Characterizing different types of O antigen structural motifs is essential for understanding the persistence/survival mechanisms and inflammatory potential of B. pseudomallei.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Vanitha Mariappan, Muttiah Barathan, Ahmad Khusairy Zulpa, Jamuna Vadivelu, Kumutha Malar Vellasamy
Summary: This study compared the adherence, invasion, survival, and biofilm production abilities of small colony variants (SCVs) and wild-type B. pseudomallei. Additionally, the proteins responsible for pathogenicity were identified through 2-DE and LC-MS analysis. The results showed that SCVs exhibited upregulation of specific proteins involved in metabolic pathways, which may contribute to the alteration of virulence factors and enhanced pathogenesis.
JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Claire Chewapreecha, Johan Pensar, Supaksorn Chattagul, Maiju Pesonen, Apiwat Sangphukieo, Phumrapee Boonklang, Chotima Potisap, Sirikamon Koosakulnirand, Edward J. Feil, Susanna Dunachie, Narisara Chantratita, Direk Limmathurotsakul, Sharon J. Peacock, Nick P. J. Day, Julian Parkhill, Nicholas R. Thomson, Rasana W. Sermswan, Jukka Corander
Summary: The research found that specific genes and non-coding RNA of the soil bacterium Burkholderia pseudomallei are under the influence of co-selection during evolution, with these genes showing significant expression correlation under physical stress conditions, and a putative adhesin playing an important role under nutrient deprivation.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Microbiology
Muhammad Shafiq, Bixia Ke, Xin Li, Mi Zeng, Yumeng Yuan, Dongmei He, Xiaoling Deng, Xiaoyang Jiao
Summary: The study identified high genetic diversity among 8 clinical Burkholderia pseudomallei isolates, which were susceptible to commonly used antibiotics for melioidosis treatment. Various antibiotic-resistant genes and mutation factors, as well as 165 virulence factors were discovered, indicating different pathogens responsible for various disorders. Phylogenetic analysis showed diverse strains with implications for disease management.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Yanshuang Wang, Xuemiao Li, David A B Dance, Han Xia, Chen Chen, Nini Luo, Anyang Li, Yanmei Li, Qiao Zhu, Qinghui Sun, Xingyong Wu, Yingfei Zeng, Lin Chen, Shen Tian, Qianfeng Xia
Summary: The study evaluated the clinical potential of Burkholderia pseudomallei phages isolated from Hainan, China, for treating melioidosis. Results showed that the phage exhibited promising lytic properties and stability, significantly improving cell and nematode survival rates.
INFECTIOUS DISEASES OF POVERTY
(2022)
Letter
Immunology
Courtney M. Dewart, Francisco A. Almeida, Christine Koval, Scott Nowicki, Jay E. Gee, Mindy Glass Elrod, Christopher A. Gulvik, Johanna S. Salzer, Sietske de Fijter, Lindy Liu
Summary: Phylogenetic analysis of a clinical isolate associated with subclinical Burkholderia pseudomallei infection revealed probable exposure in the British Virgin Islands, where reported infections are limited. Clinicians should consider this geographic distribution when evaluating possible infection among persons with compatible travel history.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Forestry
Siyao Li, Ao Zhang, Hanqing Song, Wen Guo, Zhiying Tang, Gang Lei, Lianghua Qi
Summary: Phyllostachys edulis, a species of bamboo widely distributed in China, plays a significant role in carbon sequestration and carbon sinks at the regional level. This study analyzed the distribution of soil organic carbon (SOC) in Phyllostachys edulis forests and its relationship with geographic position, climate, vegetation, and soil characteristics. The results showed that the SOC content decreased with soil depth in subtropical areas, and climate had the most significant effect on SOC in moso bamboo forests.
Article
Immunology
Javier Sanchez-Villamil, Daniel Tapia, Grace Borlee, Bradley R. Borlee, David H. Walker, Alfredo G. Torres
Summary: The study developed in vitro and in vivo models to investigate gastrointestinal infection by Burkholderia pseudomallei and identified the type 6 secretion system and type 1 fimbriae as important virulence factors. Mannose-sensitive type 1 fimbriae were found to be involved in the initial adherence of B. pseudomallei to intestinal epithelial cells, while a functional T6SS was necessary for full virulence and lethality in mice infected via the intragastric route. These findings suggest that B. pseudomallei is an enteric pathogen with T6SS playing a crucial role in gastrointestinal infection.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Watcharaporn Kamjumphol, Pisit Chareonsudjai, Sorujsiri Chareonsudjai
Article
Multidisciplinary Sciences
Chitchanok Anutrakunchai, Jan G. M. Bolscher, Bastiaan P. Krom, Sakawrat Kanthawong, Sorujsiri Chareonsudjai, Suwimol Taweechaisupapong
Article
Multidisciplinary Sciences
Rattiyaphorn Pakkulnan, Chitchanok Anutrakunchai, Sakawrat Kanthawong, Suwimol Taweechaisupapong, Pisit Chareonsudjai, Sorujsiri Chareonsudjai
Article
Multidisciplinary Sciences
Saharut Wongkaewkhiaw, Suwimol Taweechaisupapong, Sanguansak Thanaviratananich, Jan G. M. Bolscher, Kamran Nazmi, Chitchanok Anutrakunchai, Sorujsiri Chareonsudjai, Sakawrat Kanthawong
Article
Biotechnology & Applied Microbiology
Veerachat Muangsombut, Patoo Withatanung, Narisara Chantratita, Sorujsiri Chareonsudjai, Jiali Lim, Edouard E. Galyov, Orawan Ottiwet, Sineenart Sengyee, Sujintana Janesomboon, Martin J. Loessner, Matthew Dunne, Sunee Korbsrisate
Summary: Utilizing Burkholderia-specific phage tail fibers for rapid detection of B. pseudomallei has shown high sensitivity and specificity, demonstrating the potential for further development as a supplementary diagnostic tool.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Environmental Sciences
Thanyaporn Prommarach, Suradej Pholsen, Harikaranahalli Puttaiah Shivaraju, Pisit Chareonsudjai
Summary: Increasing mining and industrial discharge of untreated wastewater, excessive use of fertilizers, and heavy metal contamination in soil are serious environmental problems worldwide. This study evaluated the influence of arsenic contamination and other factors on the growth of local forage grasses. The results showed that these grasses have high tolerance to arsenic contamination and can be used for sustainable management of ecological health in mining areas.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2022)
Article
Biochemistry & Molecular Biology
Nuttaya Thonglao, Rattiyaphorn Pakkulnan, Jakkapat Paluka, Pisit Chareonsudjai, Somdej Kanokmedhakul, Kwanjai Kanokmedhakul, Sorujsiri Chareonsudjai
Summary: This study evaluates the ability of deacetylated chitosan (dCS) to disrupt biofilms of Burkholderia pseudomallei, enabling ceftazidime (CAZ) to kill the embedded bacteria. The combination of dCS and CAZ acts synergistically to kill biofilm cells more effectively than either compound alone.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Wisitsak Phoksawat, Arnone Nithichanon, Hatairat Lerdsamran, Surasakdi Wongratanacheewin, Atibordee Meesing, Chonlatip Pipattanaboon, Sakawrat Kanthawong, Sirinart Aromseree, Umaporn Yordpratum, Marut Laohaviroj, Viraphong Lulitanond, Sorujsiri Chareonsudjai, Pilaipan Puthavathana, Ludthawun Kamuthachad, Chatcharin Kamsom, Chakrit Thapphan, Kanin Salao, Arunya Chonlapan, Punnapat Nawawishkarun, Jarunee Prasertsopon, Hans J. Overgaard, Steven W. Edwards, Supranee Phanthanawiboon
Summary: The study showed that CoronaVac mainly activated antibody and certain T cell responses in the short term, but lacked long-lasting memory T cell responses, providing only short-lived protection, suggesting that a third booster dose of vaccine may improve protection.
Article
Immunology
Muthita Khamwong, Supranee Phanthanawiboon, Kanin Salao, Sorujsiri Chareonsudjai
Summary: This study compared the response of neutrophils to different strains of B. pseudomallei in terms of NET formation, revealing that biofilm-associated infections may facilitate pathogen evasion from killing mechanisms and exacerbate disease severity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Rattiyaphorn Pakkulnan, Nuttaya Thonglao, Sorujsiri Chareonsudjai
Summary: Biofilm-associated Burkholderia pseudomallei infection contributes to antibiotic resistance and relapse of melioidosis. DNase I with ceftazidime (CAZ) inhibits biofilm formation and reduces the number of viable biofilm cells. DNase I supplemented with chitosan (CS) and CAZ eradicates both shedding planktonic and embedded biofilm cells.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Pohnratchada Mattrasongkram, Saharut Wongkaewkhiaw, Suwimol Taweechaisupapong, Sorujsiri Chareonsudjai, Teerasit Techawiwattanaboon, Thararin Ngamsiri, Sakawrat Kanthawong
Summary: The study found that pretreatment with 1 alpha,25(OH)(2)D-3 can reduce the internalization of B. pseudomallei and decrease the formation of MNGCs in A549 cells. In addition, 1 alpha,25(OH)(2)D-3 can enhance the expression of specific genes and reduce inflammation. Therefore, this study suggests that supplementation with 1 alpha,25(OH)(2)D-3 may be an effective supportive treatment for melioidosis patients.
Article
Multidisciplinary Sciences
Chatruthai Meethai, Muthita Vanaporn, Narin Intarak, Varintip Lerdsittikul, Patoo Withatanung, Sujintana Janesomboon, Paiboon Vattanaviboon, Sorujsiri Chareonsudjai, Toby Wilkinson, Mark P. Stevens, Joanne M. Stevens, Sunee Korbsrisate
Summary: Burkholderia pseudomallei is a facultative intracellular bacterial pathogen that causes melioidosis, a severe invasive disease of humans. The stress-related catecholamine hormone epinephrine enhances motility of B. pseudomallei, transcription of flagellar genes and the production of flagellin.
Article
Multidisciplinary Sciences
Rujirat Hatrongjit, Parichart Boueroy, Piroon Jenjaroenpun, Thidathip Wongsurawat, Nattakan Meekhanon, Peechanika Chopjitt, Han Zheng, Nahuel Fittipaldi, Sorujsiri Chareonsudjai, Mariela Segura, Marcelo Gottschalk, Anusak Kerdsin
Summary: In this study, genomic analysis of S. suis CC94 serotype 4 strains was performed, revealing their potential pathogenicity and virulence. These strains clustered within minimum core genome group 3 and had a high number of virulence-associated genes similar to serotype 2 strains. Cytotoxicity tests showed significant toxicity of these strains towards human and pig cells. Additionally, common antimicrobial resistance genes were found in these strains. Monitoring of these potentially pathogenic strains is recommended.
Review
Ecology
Suriyanarayanan Sarvajayakesavalu, Yonglong Lu, Paul. J. A. Withers, Paulo Sergio Pavinato, Gang Pan, Pisit Chareonsudjai
ECOSYSTEM HEALTH AND SUSTAINABILITY
(2018)
